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Strain Name:
C57BL/6J-MtgxR5158Btlr/Mmmh
Stock Number:
042740-MU
Citation ID:
RRID:MMRRC_042740-MU
Other Names:
R5158 (G1), C57BL/6J-MtgxR5158Btlr
Major Collection:

Strain Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Ngf
Name: nerve growth factor
Synonyms: Ngfb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18049
HGNC: HGNC:7808
Homologene: 1876
Ptdss2
Name: phosphatidylserine synthase 2
Synonyms: PSS2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27388
Homologene: 8462
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Ctdspl2
Name: CTD small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329506
Homologene: 32311
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 14,881,661 bp
  • A to T, chromosome 1 at 16,107,997 bp
  • T to A, chromosome 1 at 55,444,631 bp
  • T to C, chromosome 1 at 134,886,428 bp
  • T to C, chromosome 1 at 138,175,084 bp
  • A to G, chromosome 1 at 169,933,009 bp
  • T to A, chromosome 2 at 24,192,786 bp
  • G to A, chromosome 2 at 29,978,443 bp
  • T to A, chromosome 2 at 121,981,293 bp
  • C to T, chromosome 2 at 130,441,279 bp
  • A to T, chromosome 3 at 102,520,129 bp
  • T to C, chromosome 4 at 19,878,292 bp
  • T to A, chromosome 4 at 25,620,731 bp
  • T to G, chromosome 4 at 107,375,165 bp
  • A to T, chromosome 4 at 113,742,212 bp
  • T to G, chromosome 4 at 118,456,684 bp
  • T to C, chromosome 4 at 126,687,090 bp
  • T to A, chromosome 4 at 135,765,586 bp
  • T to G, chromosome 4 at 138,833,284 bp
  • A to G, chromosome 5 at 8,959,176 bp
  • A to G, chromosome 5 at 65,808,462 bp
  • A to T, chromosome 5 at 87,587,594 bp
  • A to T, chromosome 5 at 100,393,321 bp
  • A to T, chromosome 5 at 125,415,152 bp
  • G to A, chromosome 7 at 7,291,619 bp
  • T to C, chromosome 7 at 15,627,309 bp
  • T to C, chromosome 7 at 80,743,068 bp
  • A to T, chromosome 7 at 120,253,429 bp
  • T to C, chromosome 7 at 141,151,771 bp
  • A to G, chromosome 8 at 13,009,715 bp
  • T to G, chromosome 8 at 45,350,435 bp
  • T to C, chromosome 9 at 73,022,401 bp
  • T to C, chromosome 10 at 26,303,688 bp
  • T to C, chromosome 10 at 93,065,197 bp
  • T to A, chromosome 10 at 107,534,704 bp
  • A to G, chromosome 11 at 12,256,198 bp
  • T to C, chromosome 11 at 53,588,751 bp
  • G to A, chromosome 11 at 58,889,845 bp
  • T to C, chromosome 11 at 70,945,005 bp
  • A to G, chromosome 11 at 70,947,719 bp
  • T to C, chromosome 12 at 115,692,101 bp
  • A to G, chromosome 14 at 21,669,986 bp
  • A to T, chromosome 14 at 75,771,516 bp
  • T to C, chromosome 14 at 123,515,737 bp
  • T to C, chromosome 15 at 102,217,029 bp
  • T to C, chromosome 17 at 29,641,254 bp
  • C to T, chromosome 17 at 33,918,449 bp
  • G to A, chromosome 17 at 34,911,664 bp
  • T to A, chromosome 17 at 38,175,454 bp
  • C to T, chromosome 17 at 45,514,829 bp
  • A to G, chromosome 18 at 80,137,543 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5158 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.