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Strain Name:
C57BL/6J-MtgxR5161Btlr/Mmmh
Stock Number:
042743-MU
Citation ID:
RRID:MMRRC_042743-MU
Other Names:
R5161 (G1), C57BL/6J-MtgxR5161Btlr
Major Collection:

Strain Information

Qki
Name: quaking, KH domain containing RNA binding
Synonyms: l(17)-1Wis, QkI, l17Wis1, 1110003F05Rik, Qk
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19317
VEGA: 17
Homologene: 11059
Atf2
Name: activating transcription factor 2
Synonyms: mXBP, CRE-BP, Creb2, D130078H02Rik, ATF-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11909
HGNC: HGNC:784
Homologene: 31061
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Macroh2a1
Name: macroH2A.1 histone
Synonyms: mH2a1, MACROH2A1.2, H2AF12M, H2afy
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66865
Homologene: 6078
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 9,622,677 bp
  • C to T, chromosome 1 at 119,707,863 bp
  • A to G, chromosome 1 at 194,751,404 bp
  • T to C, chromosome 2 at 24,858,195 bp
  • T to C, chromosome 2 at 26,395,171 bp
  • C to T, chromosome 2 at 26,636,248 bp
  • G to A, chromosome 2 at 26,993,008 bp
  • C to T, chromosome 2 at 30,108,239 bp
  • A to G, chromosome 2 at 65,764,591 bp
  • A to G, chromosome 2 at 66,229,023 bp
  • A to C, chromosome 2 at 73,829,790 bp
  • T to C, chromosome 2 at 79,878,144 bp
  • A to C, chromosome 2 at 127,141,857 bp
  • A to T, chromosome 2 at 130,663,376 bp
  • A to T, chromosome 2 at 150,255,215 bp
  • G to C, chromosome 2 at 155,632,373 bp
  • C to A, chromosome 2 at 172,432,324 bp
  • G to T, chromosome 5 at 63,898,001 bp
  • C to T, chromosome 5 at 134,263,731 bp
  • A to T, chromosome 6 at 24,891,603 bp
  • T to C, chromosome 6 at 57,258,512 bp
  • A to T, chromosome 6 at 130,482,905 bp
  • C to A, chromosome 7 at 44,396,015 bp
  • T to C, chromosome 7 at 86,364,338 bp
  • A to T, chromosome 7 at 100,998,929 bp
  • A to T, chromosome 7 at 103,250,372 bp
  • G to A, chromosome 7 at 134,734,062 bp
  • A to T, chromosome 7 at 142,377,144 bp
  • A to G, chromosome 8 at 44,952,512 bp
  • A to C, chromosome 8 at 70,888,848 bp
  • A to T, chromosome 8 at 105,268,906 bp
  • A to G, chromosome 8 at 123,235,549 bp
  • T to A, chromosome 9 at 35,741,588 bp
  • T to C, chromosome 9 at 104,124,028 bp
  • G to T, chromosome 9 at 110,629,868 bp
  • A to G, chromosome 11 at 30,024,467 bp
  • T to C, chromosome 11 at 59,028,604 bp
  • A to G, chromosome 11 at 59,064,310 bp
  • C to T, chromosome 11 at 109,673,370 bp
  • C to T, chromosome 12 at 32,204,978 bp
  • G to T, chromosome 12 at 86,267,176 bp
  • T to A, chromosome 12 at 88,012,117 bp
  • C to A, chromosome 12 at 111,722,774 bp
  • T to C, chromosome 12 at 113,736,157 bp
  • T to C, chromosome 13 at 56,089,781 bp
  • G to A, chromosome 14 at 32,193,925 bp
  • G to T, chromosome 15 at 10,551,550 bp
  • G to T, chromosome 15 at 82,063,341 bp
  • A to G, chromosome 15 at 97,757,725 bp
  • T to A, chromosome 15 at 101,450,468 bp
  • T to C, chromosome 16 at 17,276,879 bp
  • G to A, chromosome 16 at 32,762,521 bp
  • G to A, chromosome 16 at 91,908,838 bp
  • A to T, chromosome 17 at 10,215,490 bp
  • A to C, chromosome 17 at 21,691,927 bp
  • A to T, chromosome 17 at 56,882,888 bp
  • T to G, chromosome 18 at 11,722,114 bp
  • A to T, chromosome 19 at 12,298,789 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5161 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042743-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.