Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5165Btlr/Mmmh
Stock Number:
042746-MU
Citation ID:
RRID:MMRRC_042746-MU
Other Names:
R5165 (G1), C57BL/6J-MtgxR5165Btlr
Major Collection:

Strain Information

Atp1a1
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11928
HGNC: HGNC:799
Homologene: 564
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Dapp1
Name: dual adaptor for phosphotyrosine and 3-phosphoinositides 1
Synonyms: Bam32
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26377
Homologene: 32138
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30940
VEGA: 16
Homologene: 8374
Gstcd
Name: glutathione S-transferase, C-terminal domain containing
Synonyms: 4933434L15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67553
Homologene: 11693
Fnbp4
Name: formin binding protein 4
Synonyms: FBP30
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 55935
Homologene: 9087
Ralgapb
Name: Ral GTPase activating protein, beta subunit (non-catalytic)
Synonyms: B230339M05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228850
Homologene: 10666
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 57,959,236 bp
  • A to G, chromosome 1 at 155,928,368 bp
  • A to G, chromosome 1 at 163,722,523 bp
  • A to T, chromosome 1 at 172,323,157 bp
  • G to A, chromosome 1 at 173,776,565 bp
  • A to T, chromosome 1 at 192,846,822 bp
  • T to A, chromosome 2 at 24,958,189 bp
  • T to A, chromosome 2 at 76,776,556 bp
  • C to G, chromosome 2 at 90,777,657 bp
  • T to A, chromosome 2 at 111,257,223 bp
  • A to G, chromosome 2 at 158,465,912 bp
  • G to A, chromosome 3 at 27,241,528 bp
  • G to A, chromosome 3 at 32,720,208 bp
  • A to G, chromosome 3 at 81,999,591 bp
  • A to G, chromosome 3 at 101,581,789 bp
  • A to C, chromosome 3 at 133,084,679 bp
  • T to C, chromosome 3 at 137,939,215 bp
  • A to T, chromosome 4 at 112,865,668 bp
  • T to C, chromosome 5 at 120,557,945 bp
  • T to A, chromosome 6 at 29,736,078 bp
  • A to C, chromosome 6 at 69,016,665 bp
  • T to G, chromosome 6 at 71,967,925 bp
  • T to A, chromosome 6 at 83,526,832 bp
  • G to A, chromosome 7 at 19,078,035 bp
  • A to G, chromosome 7 at 19,713,667 bp
  • T to C, chromosome 7 at 38,567,405 bp
  • T to A, chromosome 7 at 144,409,636 bp
  • T to A, chromosome 8 at 23,153,743 bp
  • A to T, chromosome 8 at 24,967,174 bp
  • T to C, chromosome 8 at 94,779,876 bp
  • T to C, chromosome 9 at 106,241,255 bp
  • T to C, chromosome 9 at 107,997,630 bp
  • A to G, chromosome 10 at 80,315,850 bp
  • A to T, chromosome 10 at 117,046,060 bp
  • A to G, chromosome 11 at 49,444,376 bp
  • A to G, chromosome 11 at 69,324,234 bp
  • A to T, chromosome 11 at 83,017,127 bp
  • T to C, chromosome 11 at 83,990,935 bp
  • T to C, chromosome 11 at 118,169,082 bp
  • T to C, chromosome 12 at 61,839,624 bp
  • A to G, chromosome 13 at 54,378,976 bp
  • A to G, chromosome 13 at 58,263,196 bp
  • A to T, chromosome 15 at 4,053,769 bp
  • A to G, chromosome 15 at 39,872,461 bp
  • A to G, chromosome 15 at 54,749,189 bp
  • A to G, chromosome 15 at 77,956,012 bp
  • A to T, chromosome 15 at 90,956,376 bp
  • T to A, chromosome 15 at 102,984,000 bp
  • T to A, chromosome 16 at 11,420,775 bp
  • T to A, chromosome 16 at 15,678,272 bp
  • T to A, chromosome 16 at 77,076,405 bp
  • A to G, chromosome 17 at 37,924,361 bp
  • A to G, chromosome 18 at 20,277,023 bp
  • A to G, chromosome 18 at 37,675,670 bp
  • T to C, chromosome 19 at 5,475,411 bp
  • T to C, chromosome 19 at 9,015,665 bp
  • A to T, chromosome 19 at 12,901,200 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5165 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042746-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.