Strain Name:
C57BL/6J-MtgxR5211Btlr/Mmmh
Stock Number:
042785-MU
Citation ID:
RRID:MMRRC_042785-MU
Other Names:
R5211 (G1), C57BL/6J-MtgxR5211Btlr
Major Collection:

Gene Information

Jmjd1c
Name: jumonji domain containing 1C
Synonyms: D630035I23Rik, 5430433L24Rik, TRIP8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 108829
Homologene: 3129
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: Tas, MAPKKK4, Mekk4, MTK1, D17Rp17e, RP17, D17Rp17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Dock6
Name: dedicator of cytokinesis 6
Synonyms: 4931431C02Rik, C330023D02Rik, 2410095B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319899
VEGA: 9
Homologene: 83291
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: Gcn1l1, GCN1L, G431004K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22222
Homologene: 7582
Ascc2
Name: activating signal cointegrator 1 complex subunit 2
Synonyms: 2610034L15Rik, ASC1p100, 1700011I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75452
Homologene: 41774
Bmp2
Name: bone morphogenetic protein 2
Synonyms: Bmp2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12156
HGNC: HGNC:1069
Homologene: 926
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Syt17
Name: synaptotagmin XVII
Synonyms: Bk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 110058
Homologene: 9553
Sympk
Name: symplekin
Synonyms: 4632415H16Rik, 1500016F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68188
Homologene: 37969
Slmap
Name: sarcolemma associated protein
Synonyms: Miranda, Slap, D330001L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 83997
Homologene: 31428
9930012K11Rik
Name: RIKEN cDNA 9930012K11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 268759
Homologene: 19540
Adgrf5
Name: adhesion G protein-coupled receptor F5
Synonyms: 8430401C09Rik, Gpr116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224792
VEGA: 17
Homologene: 9065
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, dominant brown, Oca4, blanc-sale, Matp, Aim1, bls, Aim-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Nyap2
Name: neuronal tyrosine-phophorylated phosphoinositide 3-kinase adaptor 2
Synonyms: 9430031J16Rik, Jr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241134
Homologene: 18239
Vmn2r100
Name: vomeronasal 2, receptor 100
Synonyms: EG627537
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627537
Homologene: 129750
Kcnn3
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Synonyms: SK3, small conductance calcium-activated potassium channel 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140493
HGNC: HGNC:6292
Homologene: 20516
Gm7271
Name: predicted gene 7271
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 639545
Nlrp9b
Name: NLR family, pyrin domain containing 9B
Synonyms: Nalp-delta, Nalp9b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243874
Homologene: 18530
Syde2
Name: synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
Synonyms: C430017H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214804
Homologene: 19035
Krt18
Name: keratin 18
Synonyms: Endo B, K18, Krt1-18, CK18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16668
VEGA: 15
HGNC: HGNC:6430
Homologene: 55448
Gjc2
Name: gap junction protein, gamma 2
Synonyms: Gja12, connexin 47, Cx47, B230382L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 118454
Homologene: 10715
Ces2b
Name: carboxyesterase 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234669
HGNC: HGNC:1864
Homologene: 135851
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237711
Homologene: 104282
Olfml3
Name: olfactomedin-like 3
Synonyms: HNOEL-iso, ONT3, mONT3, 2810002E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99543
Homologene: 10613
Myrfl
Name: myelin regulatory factor-like
Synonyms: Gm239, LOC237558
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237558
VEGA: 10
Homologene: 88588
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: gp115, EMILIN-1, 5830419M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100952
Homologene: 5117
Kdm4d
Name: lysine (K)-specific demethylase 4D
Synonyms: Jmjd2d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244694
Homologene: 69244
Pcdhb1
Name: protocadherin beta 1
Synonyms: PcdhbA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93872
HGNC: HGNC:8680
Homologene: 8348
Cntn5
Name: contactin 5
Synonyms: 6720426O10Rik, A830025P08Rik, LOC244683, NB-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244682
HGNC: HGNC:2175
Homologene: 28447
Mx2
Name: MX dynamin-like GTPase 2
Synonyms: Mx-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17858
Itm2c
Name: integral membrane protein 2C
Synonyms: Bricd2c, BRI3, 3110038L02Rik, ITM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 64294
HGNC: HGNC:6175
Homologene: 11186
Mrgprh
Name: MAS-related GPR, member H
Synonyms: Gpr90, MrgH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 80978
VEGA: 17
Homologene: 12758
Fam71f2
Name: family with sequence similarity 71, member F2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 245884
Homologene: 52974
Lrrc8d
Name: leucine rich repeat containing 8D
Synonyms: Lrrc5, 4930525N13Rik, 2810473G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231549
Homologene: 10004
Olfr745
Name: olfactory receptor 745
Synonyms: GA_x6K02T2PMLR-6361495-6362481, MOR106-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258296
Homologene: 27116
Ly6f
Name: lymphocyte antigen 6 complex, locus F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17071
Homologene: 113718
Esd
Name: esterase D/formylglutathione hydrolase
Synonyms: Es-10, Esd, Es10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13885
HGNC: HGNC:3465
Homologene: 55623
Sbk2
Name: SH3-binding domain kinase family, member 2
Synonyms: LOC381836
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381836
Homologene: 30927
Pm20d1
Name: peptidase M20 domain containing 1
Synonyms: 4732466D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212933
Homologene: 65049
Gfy
Name: golgi-associated olfactory signaling regulator
Synonyms: Goofy, Gm581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100039953
Homologene: 85248
H3c4
Name: H3 clustered histone 4
Synonyms: H3-b, Hist1h3d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319149
HGNC: HGNC:4766
Homologene: 134332
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 81,087,274 bp
  • T to A, chromosome 1 at 85,906,528 bp
  • T to A, chromosome 1 at 131,806,909 bp
  • T to C, chromosome 2 at 120,893,170 bp
  • A to T, chromosome 2 at 133,554,630 bp
  • A to G, chromosome 3 at 89,521,231 bp
  • A to T, chromosome 3 at 103,737,199 bp
  • T to C, chromosome 3 at 146,001,338 bp
  • C to T, chromosome 5 at 30,918,393 bp
  • C to A, chromosome 5 at 76,516,403 bp
  • G to A, chromosome 5 at 105,813,740 bp
  • T to A, chromosome 5 at 115,619,312 bp
  • A to T, chromosome 6 at 29,286,099 bp
  • A to G, chromosome 7 at 4,962,967 bp
  • A to G, chromosome 7 at 19,035,889 bp
  • G to A, chromosome 7 at 20,049,456 bp
  • C to A, chromosome 7 at 45,177,858 bp
  • T to C, chromosome 7 at 118,442,403 bp
  • C to T, chromosome 8 at 104,835,063 bp
  • A to T, chromosome 9 at 9,704,889 bp
  • A to C, chromosome 9 at 14,463,104 bp
  • T to C, chromosome 9 at 21,820,352 bp
  • A to C, chromosome 9 at 119,661,232 bp
  • C to G, chromosome 10 at 67,232,016 bp
  • C to T, chromosome 10 at 116,798,630 bp
  • T to C, chromosome 11 at 4,673,399 bp
  • T to C, chromosome 11 at 29,854,145 bp
  • A to G, chromosome 11 at 59,177,458 bp
  • G to T, chromosome 13 at 23,575,841 bp
  • T to C, chromosome 14 at 26,482,962 bp
  • C to T, chromosome 14 at 50,643,253 bp
  • A to G, chromosome 14 at 70,156,784 bp
  • T to C, chromosome 14 at 74,741,192 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • G to A, chromosome 15 at 75,271,803 bp
  • T to G, chromosome 15 at 102,031,453 bp
  • A to T, chromosome 16 at 97,547,433 bp
  • A to G, chromosome 17 at 12,232,434 bp
  • T to C, chromosome 17 at 12,877,002 bp
  • A to G, chromosome 17 at 19,525,995 bp
  • A to T, chromosome 17 at 43,422,620 bp
  • G to A, chromosome 18 at 37,266,651 bp
  • T to A, chromosome 19 at 36,996,562 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5211 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042785-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.