Strain Name:
C57BL/6J-MtgxR5221Btlr/Mmmh
Stock Number:
042794-MU
Citation ID:
RRID:MMRRC_042794-MU
Other Names:
R5221 (G1), C57BL/6J-MtgxR5221Btlr
Major Collection:

Gene Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 24069
Homologene: 9262
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242022
Homologene: 18454
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Ptprf
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Ppp1r1a
Name: protein phosphatase 1, regulatory inhibitor subunit 1A
Synonyms: I-1, protein phosphatase inhibitor-1, inhibitor-1, 0610038N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 58200
VEGA: 15
HGNC: HGNC:9286
Homologene: 4905
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Lin9
Name: lin-9 homolog (C. elegans)
Synonyms: 2700022J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72568
Homologene: 35252
Plxna1
Name: plexin A1
Synonyms: NOV, Plxn1, 2600013D04Rik, PlexA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18844
HGNC: HGNC:9099
Homologene: 56426
Zfp462
Name: zinc finger protein 462
Synonyms: Gt4-2, 9430078C22Rik, Zfpip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242466
Homologene: 41430
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545389
Homologene: 22844
Sltm
Name: SAFB-like, transcription modulator
Synonyms: 5730455C01Rik, 9130215G10Rik, 5730555F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66660
VEGA: 9
Homologene: 11696
Ubtf
Name: upstream binding transcription factor, RNA polymerase I
Synonyms: UBF1, Tcfubf, UBF, A930005G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21429
Homologene: 7970
Mafa
Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Synonyms: RIPE3b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 378435
VEGA: 15
Homologene: 65867
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Tpbg
Name: trophoblast glycoprotein
Synonyms: 5T4, 5T4 oncofetal antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21983
Homologene: 4859
Rmdn3
Name: regulator of microtubule dynamics 3
Synonyms: 1200015F23Rik, Fam82a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67809
Homologene: 34926
Tmem68
Name: transmembrane protein 68
Synonyms: 2010300G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72098
Homologene: 12440
Wscd1
Name: WSC domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216881
Homologene: 18590
Rai1
Name: retinoic acid induced 1
Synonyms: Gt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19377
HGNC: HGNC:9834
Homologene: 7508
Trappc14
Name: trafficking protein particle complex 14
Synonyms: Map11, BC037034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231807
Homologene: 10106
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Ccser1
Name: coiled-coil serine rich 1
Synonyms: 6230405M12Rik, C130092O11Rik, Fam190a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232035
Homologene: 28086
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Farp2
Name: FERM, RhoGEF and pleckstrin domain protein 2
Synonyms: Fir, D030026M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227377
Homologene: 8877
C8a
Name: complement component 8, alpha polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230558
HGNC: HGNC:1352
Homologene: 472
Coq2
Name: coenzyme Q2 4-hydroxybenzoate polyprenyltransferase
Synonyms: 2310002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71883
Homologene: 69192
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230622
Homologene: 135888
Antkmt
Name: adenine nucleotide translocase lysine methyltransferase
Synonyms: Fam173a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214917
VEGA: 17
Homologene: 11398
Col4a2
Name: collagen, type IV, alpha 2
Synonyms: Col4a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12827
HGNC: HGNC:2203
Homologene: 1390
Gmip
Name: Gem-interacting protein
Synonyms: 5031419I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78816
Homologene: 9570
Dsg1a
Name: desmoglein 1 alpha
Synonyms: Dsg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13510
HGNC: HGNC:3048
Homologene: 1463
Bean1
Name: brain expressed, associated with Nedd4, 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 65115
Homologene: 110172
Olfr1026
Name: olfactory receptor 1026
Synonyms: GA_x6K02T2Q125-47402610-47403533, MOR196-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258577
Homologene: 73971
Asap2
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms: LOC385250, Ddef2, 6530401G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211914
HGNC: HGNC:2721
Homologene: 2888
Slc22a27
Name: solute carrier family 22, member 27
Synonyms: mOAT6 related protein, AB056442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 171405
Homologene: 77136
Arhgap26
Name: Rho GTPase activating protein 26
Synonyms: 1810044B20Rik, 2610010G17Rik, 4933432P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71302
Homologene: 36349
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: LOC384387, C330043M08Rik, Fam188b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330323
Homologene: 49991
Gstp3
Name: glutathione S-transferase pi 3
Synonyms: BC021614
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225884
HGNC: HGNC:4638
Homologene: 129928
Gbp8
Name: guanylate-binding protein 8
Synonyms: 5830443L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76074
Gm9774
Name: predicted pseudogene 9774
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100043022
Vmn2r-ps158
Name: vomeronasal 2, receptor, pseudogene 158
Synonyms: Gm9268, Vmn2r126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668612
Homologene: 104832
Olfr1111
Name: olfactory receptor 1111
Synonyms: GA_x6K02T2Q125-48635468-48634530, MOR181-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258586
Homologene: 74238
Olfr1
Name: olfactory receptor 1
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Olfr912
Name: olfactory receptor 912
Synonyms: GA_x6K02T2PVTD-32283590-32284522, MOR165-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258806
VEGA: 9
Homologene: 115510
Zfp354b
Name: zinc finger protein 354B
Synonyms: Kid2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27274
Homologene: 32187
Lrrc36
Name: leucine rich repeat containing 36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270091
Homologene: 23090
Olfr709-ps1
Name: olfactory receptor 709, pseudogene 1
Synonyms: GA_x6K02T2PBJ9-9307730-9306814, MOR260-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258146
Mesp2
Name: mesoderm posterior 2
Synonyms: bHLHc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17293
Homologene: 7420
Rrh
Name: retinal pigment epithelium derived rhodopsin homolog
Synonyms: Peropsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20132
Homologene: 55977
Gvin-ps3
Name: GTPase, very large interferon inducible, pseudogene 3
Synonyms: Gm8979, Gm21884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668108
Ppp1r14a
Name: protein phosphatase 1, regulatory inhibitor subunit 14A
Synonyms: 1110001M11Rik, Cpi17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68458
Homologene: 12267
Gm6457
Name: predicted pseudogene 6457
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 623867
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 93,576,418 bp
  • T to C, chromosome 1 at 176,736,854 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • A to C, chromosome 2 at 41,112,982 bp
  • A to G, chromosome 2 at 85,924,149 bp
  • T to C, chromosome 2 at 87,150,481 bp
  • A to G, chromosome 2 at 119,156,454 bp
  • A to G, chromosome 3 at 53,585,611 bp
  • T to C, chromosome 3 at 92,428,508 bp
  • T to C, chromosome 3 at 129,815,631 bp
  • T to C, chromosome 4 at 3,560,561 bp
  • T to A, chromosome 4 at 55,016,887 bp
  • T to A, chromosome 4 at 63,993,297 bp
  • C to T, chromosome 4 at 104,845,925 bp
  • A to T, chromosome 4 at 112,894,924 bp
  • C to T, chromosome 4 at 118,225,108 bp
  • G to A, chromosome 5 at 100,657,832 bp
  • A to G, chromosome 5 at 105,050,979 bp
  • C to T, chromosome 5 at 138,262,240 bp
  • G to T, chromosome 5 at 150,989,151 bp
  • A to G, chromosome 6 at 55,224,107 bp
  • T to C, chromosome 6 at 61,312,091 bp
  • T to C, chromosome 6 at 89,321,016 bp
  • T to A, chromosome 7 at 29,289,501 bp
  • T to A, chromosome 7 at 43,023,260 bp
  • C to T, chromosome 7 at 79,811,719 bp
  • A to T, chromosome 7 at 85,221,877 bp
  • G to C, chromosome 7 at 106,083,974 bp
  • A to T, chromosome 7 at 106,927,061 bp
  • A to G, chromosome 8 at 11,448,225 bp
  • T to C, chromosome 8 at 41,288,156 bp
  • T to G, chromosome 8 at 69,814,135 bp
  • A to T, chromosome 8 at 104,215,152 bp
  • A to G, chromosome 8 at 105,443,856 bp
  • A to T, chromosome 9 at 38,581,852 bp
  • T to G, chromosome 9 at 70,579,403 bp
  • T to C, chromosome 9 at 85,844,425 bp
  • A to C, chromosome 11 at 50,923,090 bp
  • A to G, chromosome 11 at 60,190,597 bp
  • G to T, chromosome 11 at 71,768,675 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • G to A, chromosome 11 at 102,307,990 bp
  • A to G, chromosome 12 at 21,213,190 bp
  • T to A, chromosome 15 at 75,747,042 bp
  • G to A, chromosome 15 at 103,533,050 bp
  • T to A, chromosome 16 at 37,042,178 bp
  • T to C, chromosome 17 at 25,791,639 bp
  • T to C, chromosome 18 at 14,570,117 bp
  • T to A, chromosome 18 at 20,324,014 bp
  • T to A, chromosome 18 at 39,110,472 bp
  • A to G, chromosome 19 at 4,057,607 bp
  • C to G, chromosome 19 at 7,865,938 bp
  • T to C, chromosome 19 at 46,450,965 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5221 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042794-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.