Strain Name:
C57BL/6J-MtgxR5222Btlr/Mmmh
Stock Number:
042795-MU
Citation ID:
RRID:MMRRC_042795-MU
Other Names:
R5222 (G1), C57BL/6J-MtgxR5222Btlr
Major Collection:

Gene Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SW1/SNF, SNF2beta, b2b692Clo, Brg1, b2b508.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20586
Homologene: 135927
Pmel
Name: premelanosome protein
Synonyms: D12S53Eh, gp87, D10H12S53E, Si, gp100, Pmel17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20431
VEGA: 10
Homologene: 5048
Prrx1
Name: paired related homeobox 1
Synonyms: mHox, A230024N07Rik, Pmx1, mHox, K-2, Prx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
Cd40
Name: CD40 antigen
Synonyms: Cd40, Bp50, Tnfrsf5, p50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21939
Homologene: 954
Rtel1
Name: regulator of telomere elongation helicase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269400
Homologene: 13168
Dapk3
Name: death-associated protein kinase 3
Synonyms: ZIP kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13144
VEGA: 10
HGNC: HGNC:2676
Homologene: 20353
Cenpc1
Name: centromere protein C1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12617
HGNC: HGNC:1854
Homologene: 1371
Lin9
Name: lin-9 homolog (C. elegans)
Synonyms: 2700022J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72568
Homologene: 35252
Tcf7l2
Name: transcription factor 7 like 2, T cell specific, HMG box
Synonyms: mTcf-4E, Tcf4, TCF4E, TCF4B, Tcf-4, mTcf-4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21416
Homologene: 7564
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: Fam62b, 4921504I16Rik, 2410017M09Rik, D12Ertd551e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 52635
VEGA: 12
Homologene: 32699
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66622
VEGA: 12
Homologene: 11998
Sap130
Name: Sin3A associated protein
Synonyms: 2610304F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269003
VEGA: 18
Homologene: 11577
Wapl
Name: WAPL cohesin release factor
Synonyms: Wapal, A530089A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218914
Homologene: 41002
Esf1
Name: ESF1 nucleolar pre-rRNA processing protein homolog
Synonyms: 2610101J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66580
Homologene: 5717
Rif1
Name: replication timing regulatory factor 1
Synonyms: 5730435J01Rik, D2Ertd145e, 6530403D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51869
Homologene: 41231
Rad17
Name: RAD17 checkpoint clamp loader component
Synonyms: 9430035O09Rik, MmRad24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19356
VEGA: 13
HGNC: HGNC:9807
Homologene: 32117
Cit
Name: citron
Synonyms: C030025P15Rik, Cit-k, citron kinase, CRIK-SK, citron-N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12704
HGNC: HGNC:1985
Homologene: 21404
Vps8
Name: VPS8 CORVET complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209018
Homologene: 44592
Enpp7
Name: ectonucleotide pyrophosphatase/phosphodiesterase 7
Synonyms: Alk-SMase, LOC238011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 238011
Homologene: 110852
Dgke
Name: diacylglycerol kinase, epsilon
Synonyms: DAGK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56077
HGNC: HGNC:2852
Homologene: 2705
Sec31a
Name: Sec31 homolog A (S. cerevisiae)
Synonyms: 1810024J13Rik, Sec31l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69162
Homologene: 42056
Vrk3
Name: vaccinia related kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101568
Homologene: 9509
Pdcd1
Name: programmed cell death 1
Synonyms: Pdc1, PD-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18566
HGNC: HGNC:8760
Homologene: 3681
Ttn
Name: titin
Synonyms: mdm, 2310036G12Rik, D830007G01Rik, connectin, 1100001C23Rik, shru, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Arhgap30
Name: Rho GTPase activating protein 30
Synonyms: 6030405P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226652
Homologene: 18766
Ptprq
Name: protein tyrosine phosphatase, receptor type, Q
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Uspl1
Name: ubiquitin specific peptidase like 1
Synonyms: E430026A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231915
Homologene: 4235
Spaca6
Name: sperm acrosome associated 6
Synonyms: 4930546H06Rik, B230206P06Rik, Ncrna00085
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75202
Homologene: 53443
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: OTTMUSG00000005786, LOC380698
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Gm6878
Name: predicted gene 6878
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 628416
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: 2900051M01Rik, Glur-1, GluR-A, GluR1, GluRA, Glr-1, HIPA1, Glr1, Glur1, GluA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234311
Homologene: 23031
Acad11
Name: acyl-Coenzyme A dehydrogenase family, member 11
Synonyms: 5730439E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102632
Homologene: 49896
Slco6b1
Name: solute carrier organic anion transporter family, member 6b1
Synonyms: 1700022M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67854
Homologene: 12199
Arhgef33
Name: Rho guanine nucleotide exchange factor (GEF) 33
Synonyms: Gm941, LOC381112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381112
VEGA: 17
Homologene: 55190
Pramel28
Name: PRAME like 28
Synonyms: Gm13101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 626922
Angpt1
Name: angiopoietin 1
Synonyms: 1110046O21Rik, Angiopoietin-1, ang1, Ang-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11600
VEGA: 15
HGNC: HGNC:484
Homologene: 37447
Mark1
Name: MAP/microtubule affinity regulating kinase 1
Synonyms: B930025N23Rik, Emk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226778
HGNC: HGNC:6896
Homologene: 49552
Olfr1370
Name: olfactory receptor 1370
Synonyms: GA_x6K02T2QHY8-12181473-12182423, MOR256-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 258528
Homologene: 105231
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaN, SNS2, NSS2, NaT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Slc5a9
Name: solute carrier family 5 (sodium/glucose cotransporter), member 9
Synonyms: SGLT4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230612
Homologene: 17081
Olfr166
Name: olfactory receptor 166
Synonyms: MOR270-1, GA_x54KRFPKG5P-15934912-15935850
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259071
Homologene: 88350
Olfr1
Name: olfactory receptor 1
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Rpp14
Name: ribonuclease P 14 subunit
Synonyms: 2610511E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67053
Homologene: 5113
Epm2a
Name: epilepsy, progressive myoclonic epilepsy, type 2 gene alpha
Synonyms: Tg(TcraK,TcrbK)TG-BFlv, TG-B, laforin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13853
HGNC: HGNC:3413
Homologene: 38087
4930523C07Rik
Name: RIKEN cDNA 4930523C07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67647
Homologene: 124386
Tagap
Name: T cell activation Rho GTPase activating protein
Synonyms: tcs-1, Tcd1a, Tcd-1, Tcd1, TRD, tcs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72536
Homologene: 44943
Pstpip2
Name: proline-serine-threonine phosphatase-interacting protein 2
Synonyms: cmo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 19201
VEGA: 18
HGNC: HGNC:9581
Homologene: 69150
Gm5455
Name: predicted pseudogene 5455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432803
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 24,559,640 bp
  • A to T, chromosome 1 at 94,052,450 bp
  • T to A, chromosome 1 at 96,997,491 bp
  • C to A, chromosome 1 at 160,044,608 bp
  • C to T, chromosome 1 at 163,261,973 bp
  • T to A, chromosome 1 at 171,385,257 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • A to T, chromosome 1 at 184,928,091 bp
  • T to C, chromosome 2 at 52,077,020 bp
  • A to G, chromosome 2 at 76,878,853 bp
  • A to G, chromosome 2 at 140,158,583 bp
  • G to C, chromosome 2 at 165,066,544 bp
  • G to T, chromosome 2 at 181,346,983 bp
  • T to A, chromosome 4 at 111,898,611 bp
  • T to A, chromosome 4 at 143,964,792 bp
  • A to T, chromosome 5 at 86,037,747 bp
  • G to T, chromosome 5 at 100,382,895 bp
  • C to A, chromosome 5 at 115,952,543 bp
  • C to A, chromosome 5 at 149,214,101 bp
  • A to T, chromosome 7 at 44,759,796 bp
  • T to C, chromosome 8 at 61,984,158 bp
  • A to G, chromosome 9 at 21,655,706 bp
  • G to A, chromosome 9 at 104,097,377 bp
  • A to G, chromosome 9 at 119,815,202 bp
  • A to G, chromosome 10 at 11,448,749 bp
  • A to G, chromosome 10 at 81,192,460 bp
  • A to G, chromosome 10 at 107,662,564 bp
  • A to G, chromosome 10 at 128,718,984 bp
  • T to A, chromosome 11 at 57,189,797 bp
  • T to A, chromosome 11 at 59,044,145 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • G to T, chromosome 11 at 89,050,394 bp
  • A to G, chromosome 11 at 118,990,962 bp
  • A to T, chromosome 12 at 102,775,705 bp
  • T to C, chromosome 12 at 116,318,826 bp
  • T to A, chromosome 13 at 21,072,569 bp
  • G to A, chromosome 13 at 100,633,891 bp
  • T to C, chromosome 13 at 110,304,960 bp
  • T to C, chromosome 14 at 8,087,513 bp
  • T to A, chromosome 14 at 34,736,685 bp
  • T to G, chromosome 14 at 67,313,594 bp
  • T to C, chromosome 15 at 42,676,334 bp
  • A to C, chromosome 16 at 19,486,930 bp
  • T to A, chromosome 16 at 21,581,548 bp
  • C to A, chromosome 17 at 7,933,641 bp
  • A to T, chromosome 17 at 7,933,642 bp
  • A to G, chromosome 17 at 17,838,105 bp
  • A to G, chromosome 17 at 80,337,314 bp
  • C to T, chromosome 18 at 31,666,703 bp
  • T to A, chromosome 18 at 77,874,332 bp
  • A to G, chromosome 19 at 55,898,612 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5222 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042795-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.