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Strain Name:
C57BL/6J-MtgxR5227Btlr/Mmmh
Stock Number:
042800-MU
Citation ID:
RRID:MMRRC_042800-MU
Other Names:
R5227 (G1), C57BL/6J-MtgxR5227Btlr
Major Collection:

Strain Information

Ebf2
Name: early B cell factor 2
Synonyms: O/E-3, D14Ggc1e, Mmot1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13592
Homologene: 56471
Fzd9
Name: frizzled class receptor 9
Synonyms: mfz9, frizzled 9, Fz9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14371
HGNC: HGNC:4047
Homologene: 2619
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Swt1
Name: SWT1 RNA endoribonuclease homolog (S. cerevisiae)
Synonyms: 1200016B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66875
Homologene: 32355
Ireb2
Name: iron responsive element binding protein 2
Synonyms: Irp2, D9Ertd85e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64602
VEGA: 9
HGNC: HGNC:6115
Homologene: 11280
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Kansl1
Name: KAT8 regulatory NSL complex subunit 1
Synonyms: 1700081L11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76719
Homologene: 9140
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 25,093,952 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 136,274,891 bp
  • G to A, chromosome 1 at 151,402,976 bp
  • T to G, chromosome 2 at 40,851,793 bp
  • T to A, chromosome 2 at 93,677,380 bp
  • A to G, chromosome 2 at 120,085,331 bp
  • T to C, chromosome 2 at 120,523,968 bp
  • T to A, chromosome 2 at 160,909,265 bp
  • A to G, chromosome 3 at 103,736,421 bp
  • T to C, chromosome 3 at 104,802,578 bp
  • T to G, chromosome 3 at 107,194,428 bp
  • A to G, chromosome 4 at 21,874,587 bp
  • C to T, chromosome 4 at 22,376,840 bp
  • A to G, chromosome 4 at 133,680,405 bp
  • A to T, chromosome 4 at 145,181,207 bp
  • T to C, chromosome 5 at 14,713,560 bp
  • G to A, chromosome 5 at 34,821,691 bp
  • A to G, chromosome 5 at 124,608,620 bp
  • C to A, chromosome 5 at 129,122,583 bp
  • T to C, chromosome 5 at 135,249,606 bp
  • G to C, chromosome 6 at 86,391,744 bp
  • G to A, chromosome 6 at 90,072,771 bp
  • C to T, chromosome 6 at 101,153,311 bp
  • T to C, chromosome 7 at 14,561,676 bp
  • T to C, chromosome 7 at 16,810,710 bp
  • T to C, chromosome 7 at 35,309,351 bp
  • T to C, chromosome 7 at 43,351,940 bp
  • AGGGCCAG to A, chromosome 7 at 80,098,347 bp
  • T to C, chromosome 7 at 80,313,179 bp
  • A to G, chromosome 7 at 104,704,322 bp
  • T to A, chromosome 8 at 21,861,878 bp
  • T to C, chromosome 8 at 84,074,058 bp
  • T to A, chromosome 8 at 127,410,710 bp
  • T to A, chromosome 9 at 54,896,601 bp
  • T to A, chromosome 9 at 73,012,072 bp
  • G to A, chromosome 9 at 88,398,294 bp
  • A to G, chromosome 9 at 106,986,070 bp
  • A to T, chromosome 9 at 118,047,458 bp
  • T to A, chromosome 11 at 58,778,879 bp
  • A to G, chromosome 11 at 69,736,913 bp
  • T to C, chromosome 11 at 93,924,648 bp
  • G to A, chromosome 11 at 98,516,864 bp
  • T to G, chromosome 11 at 104,356,814 bp
  • A to T, chromosome 12 at 84,152,887 bp
  • T to A, chromosome 12 at 101,884,920 bp
  • T to C, chromosome 14 at 30,815,254 bp
  • G to A, chromosome 14 at 33,941,494 bp
  • A to T, chromosome 14 at 67,247,069 bp
  • T to A, chromosome 15 at 9,007,312 bp
  • T to A, chromosome 15 at 43,251,521 bp
  • C to T, chromosome 15 at 58,581,903 bp
  • A to G, chromosome 15 at 66,759,567 bp
  • A to T, chromosome 15 at 76,598,948 bp
  • T to A, chromosome 16 at 33,763,591 bp
  • T to C, chromosome 17 at 18,049,557 bp
  • T to A, chromosome 17 at 26,759,765 bp
  • C to T, chromosome 17 at 37,070,066 bp
  • T to C, chromosome 17 at 56,717,867 bp
  • T to C, chromosome 17 at 84,691,821 bp
  • A to T, chromosome 19 at 11,068,416 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042800-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.