Strain Name:
C57BL/6J-MtgxR5228Btlr/Mmmh
Stock Number:
042801-MU
Citation ID:
RRID:MMRRC_042801-MU
Other Names:
R5228 (G1), C57BL/6J-MtgxR5228Btlr
Major Collection:

Gene Information

Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Rps8
Name: ribosomal protein S8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20116
Homologene: 133143
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243725
Homologene: 14247
Topors
Name: topoisomerase I binding, arginine/serine-rich
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 106021
Homologene: 4237
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20874
Homologene: 22515
Dapp1
Name: dual adaptor for phosphotyrosine and 3-phosphoinositides 1
Synonyms: Bam32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26377
Homologene: 32138
Pml
Name: promyelocytic leukemia
Synonyms: Trim19, 1200009E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18854
HGNC: HGNC:9113
Homologene: 13245
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 4632408O18Rik, 2900005P22Rik, Nibp, TRS130, 1810044A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Kif5b
Name: kinesin family member 5B
Synonyms: Khc, kinesin heavy chain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16573
HGNC: HGNC:6324
Homologene: 55829
Ipo7
Name: importin 7
Synonyms: RanBP7, Imp7, A330055O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233726
HGNC: HGNC:9852
Homologene: 4659
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC333789, LOC386488, B3bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 333789
Homologene: 32396
Iws1
Name: IWS1, SUPT6 interacting protein
Synonyms: 1700069O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 73473
Homologene: 134421
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Pms2
Name: PMS1 homolog2, mismatch repair system component
Synonyms: mismatch repair, DNA mismatch repair
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18861
HGNC: HGNC:9122
Homologene: 133560
Tcf20
Name: transcription factor 20
Synonyms: SPBP, stromelysin 1 PDGF responsive element binding protein, 2810438H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21411
VEGA: 15
Homologene: 4131
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108837
Homologene: 34661
Morc2a
Name: microrchidia 2A
Synonyms: 8430403M08Rik, Zcwcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74522
Homologene: 8966
Rlbp1
Name: retinaldehyde binding protein 1
Synonyms: CRALBP, 3110056M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19771
Homologene: 68046
Slc4a4
Name: solute carrier family 4 (anion exchanger), member 4
Synonyms: NBC, NBC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54403
Homologene: 55776
Hspa12b
Name: heat shock protein 12B
Synonyms: 2700081N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72630
Homologene: 41749
C1rl
Name: complement component 1, r subcomponent-like
Synonyms: C1r-LP, C1rl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232371
Homologene: 9559
Tab2
Name: TGF-beta activated kinase 1/MAP3K7 binding protein 2
Synonyms: Tak1 binding protein 2, 1110030N06Rik, A530078N03Rik, Map3k7ip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68652
Homologene: 9019
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100041194
Homologene: 131081
Kcnh4
Name: potassium voltage-gated channel, subfamily H (eag-related), member 4
Synonyms: BEC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380728
HGNC: HGNC:6253
Homologene: 8180
Galnt16
Name: polypeptide N-acetylgalactosaminyltransferase 16
Synonyms: 5730405L21Rik, Galntl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 108760
VEGA: 12
Homologene: 18907
Actn2
Name: actinin alpha 2
Synonyms: 1110008F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11472
HGNC: HGNC:164
Homologene: 31016
Ube2m
Name: ubiquitin-conjugating enzyme E2M
Synonyms: UBC12, Ubc-rs2, 2510040H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22192
Homologene: 2952
Inpp4b
Name: inositol polyphosphate-4-phosphatase, type II
Synonyms: E130107I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234515
HGNC: HGNC:6075
Homologene: 20832
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666060
Homologene: 8939
Gm6327
Name: predicted gene 6327
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Sh3rf2
Name: SH3 domain containing ring finger 2
Synonyms: RNF158, 9130023G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269016
Homologene: 17618
Clec2h
Name: C-type lectin domain family 2, member h
Synonyms: Clrf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94071
Homologene: 131164
Gtsf2
Name: gametocyte specific factor 2
Synonyms: BC048502
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223927
VEGA: 15
Homologene: 77620
Klhl40
Name: kelch-like 40
Synonyms: 2310024D23Rik, Kbtbd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72330
VEGA: 9
Homologene: 17571
Pira2
Name: paired-Ig-like receptor A2
Synonyms: 6M23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18725
Homologene: 134028
Gbp8
Name: guanylate-binding protein 8
Synonyms: 5830443L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76074
Pla2g4f
Name: phospholipase A2, group IVF
Synonyms: 4732472I07Rik, Pla2zeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271844
Homologene: 77933
Aifm2
Name: apoptosis-inducing factor, mitochondrion-associated 2
Synonyms: PRG3, D730001I10Rik, 5430437E11Rik, Amid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71361
Homologene: 6862
Gldn
Name: gliomedin
Synonyms: Crlg2, CRG-L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235379
Homologene: 14529
Vmn2r34
Name: vomeronasal 2, receptor 34
Synonyms: ENSMUSG00000070841
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042636
Homologene: 113703
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,437,609 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 118,836,206 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • C to A, chromosome 1 at 150,646,701 bp
  • G to A, chromosome 2 at 120,311,494 bp
  • T to C, chromosome 2 at 131,142,964 bp
  • T to C, chromosome 3 at 137,981,457 bp
  • G to C, chromosome 4 at 40,262,367 bp
  • T to C, chromosome 4 at 45,284,322 bp
  • G to C, chromosome 4 at 117,155,155 bp
  • G to T, chromosome 4 at 156,166,946 bp
  • T to C, chromosome 5 at 65,807,518 bp
  • A to G, chromosome 5 at 89,156,525 bp
  • G to T, chromosome 5 at 105,016,185 bp
  • T to C, chromosome 5 at 143,923,597 bp
  • G to T, chromosome 6 at 4,906,061 bp
  • C to T, chromosome 6 at 124,508,468 bp
  • G to T, chromosome 6 at 128,674,786 bp
  • T to A, chromosome 7 at 3,844,374 bp
  • T to C, chromosome 7 at 7,672,341 bp
  • G to T, chromosome 7 at 13,035,770 bp
  • T to G, chromosome 7 at 79,377,334 bp
  • T to A, chromosome 7 at 110,046,762 bp
  • A to C, chromosome 8 at 48,236,355 bp
  • C to G, chromosome 8 at 81,768,115 bp
  • C to T, chromosome 9 at 54,334,719 bp
  • G to A, chromosome 9 at 58,219,997 bp
  • C to A, chromosome 9 at 70,322,358 bp
  • T to C, chromosome 9 at 85,726,689 bp
  • A to G, chromosome 9 at 121,777,801 bp
  • A to T, chromosome 10 at 7,910,177 bp
  • T to C, chromosome 10 at 61,732,417 bp
  • T to C, chromosome 11 at 3,685,439 bp
  • T to C, chromosome 11 at 100,746,896 bp
  • A to T, chromosome 12 at 80,584,048 bp
  • A to G, chromosome 12 at 112,775,386 bp
  • T to C, chromosome 13 at 12,288,659 bp
  • A to T, chromosome 15 at 73,057,995 bp
  • G to A, chromosome 15 at 82,855,955 bp
  • A to G, chromosome 15 at 103,444,615 bp
  • T to A, chromosome 16 at 12,760,358 bp
  • C to T, chromosome 16 at 12,761,006 bp
  • G to A, chromosome 18 at 6,220,964 bp
  • A to G, chromosome 18 at 32,088,261 bp
  • T to C, chromosome 18 at 42,153,181 bp
  • A to T, chromosome 19 at 47,625,332 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042801-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.