Strain Name:
C57BL/6J-MtgxR5238Btlr/Mmmh
Stock Number:
042809-MU
Citation ID:
RRID:MMRRC_042809-MU
Other Names:
R5238 (G1), C57BL/6J-MtgxR5238Btlr
Major Collection:

Gene Information

Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68310
Homologene: 32904
Mok
Name: MOK protein kinase
Synonyms: MOK, MAPK/MAK/MRK/ overlapping kinase, Rage, Stk30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26448
HGNC: HGNC:9833
Homologene: 8062
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72567
Homologene: 8832
Uba3
Name: ubiquitin-like modifier activating enzyme 3
Synonyms: ubiquitin activating enzyme 3, A830034N06Rik, Ube1c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22200
Homologene: 2951
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: P140SRA-1, Shyc, pl-1, l(7)1Rl, Sra-1, E030028J09Rik, l7Rl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20430
Homologene: 22628
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78795
Homologene: 11847
Eed
Name: embryonic ectoderm development
Synonyms: l(7)5Rn, l7Rn5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13626
HGNC: HGNC:3188
Homologene: 2814
Slc39a1
Name: solute carrier family 39 (zinc transporter), member 1
Synonyms: zip1, Zirtl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 30791
Homologene: 40906
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211652
Homologene: 69180
Dffa
Name: DNA fragmentation factor, alpha subunit
Synonyms: ICAD-S, ICAD-L, Dff45, DFF35, A330085O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13347
HGNC: HGNC:2772
Homologene: 3240
Sphk1
Name: sphingosine kinase 1
Synonyms: 1110006G24Rik, SK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20698
Homologene: 39748
Robo3
Name: roundabout guidance receptor 3
Synonyms: Rig-1, Rbig1, Robo3b, Robo3a, Rig1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19649
Homologene: 32119
Gapdh
Name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: Gapd, Gapd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14433
HGNC: HGNC:4141
Homologene: 107053
Mcm9
Name: minichromosome maintenance 9 homologous recombination repair factor
Synonyms: 9030408O17Rik, Mcmdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71567
VEGA: 10
Homologene: 13546
Zfp600
Name: zinc finger protein 600
Synonyms: Gm13164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 667666
Homologene: 133076
Nptx2
Name: neuronal pentraxin 2
Synonyms: np2, narp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53324
HGNC: HGNC:7953
Homologene: 1892
Man2a1
Name: mannosidase 2, alpha 1
Synonyms: Mana-2, Mana2, Map-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17158
HGNC: HGNC:6824
Homologene: 1777
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Plxdc2
Name: plexin domain containing 2
Synonyms: 1200007L24Rik, Tem7r, 5430431D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67448
Homologene: 41666
Tiprl
Name: TIP41, TOR signalling pathway regulator-like (S. cerevisiae)
Synonyms: 1810011K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226591
Homologene: 7140
Atad2
Name: ATPase family, AAA domain containing 2
Synonyms: 2610509G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70472
VEGA: 15
Homologene: 6044
Cbwd1
Name: COBW domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226043
VEGA: 19
Homologene: 6843
Tmub2
Name: transmembrane and ubiquitin-like domain containing 2
Synonyms: 2010008E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72053
Homologene: 18581
Mst1r
Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms: STK, Fv-2, Ron, CDw136, friend virus susceptibility 2, PTK8, Fv2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19882
HGNC: HGNC:7381
Homologene: 1835
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
4932431P20Rik
Name: RIKEN cDNA 4932431P20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Kidins220
Name: kinase D-interacting substrate 220
Synonyms: 3110039L19Rik, C330002I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 77480
VEGA: 12
Homologene: 14254
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: Mlsn1, 4732499L03Rik, melastatin, LTRPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Vmn1r50
Name: vomeronasal 1 receptor 50
Synonyms: VN2, V1rb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113852
Homologene: 113975
Nckap5
Name: NCK-associated protein 5
Synonyms: LOC380609, D130011D22Rik, E030049G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210356
Homologene: 35542
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628870
Homologene: 46008
Abhd12b
Name: abhydrolase domain containing 12B
Synonyms: LOC328121
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100504285
Homologene: 128243
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: cytoplasmic linker protein 50, Clip50, CLIP-170, 1110007I12Rik, 4631429H07Rik, Rsn, Clip 170, restin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56430
Homologene: 74455
Slfn8
Name: schlafen 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 276950
Homologene: 45432
Ccdc188
Name: coiled-coil domain containing 188
Synonyms: Gm7873
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 102638083
VEGA: 16
Homologene: 90442
Gm12185
Name: predicted gene 12185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 620913
Homologene: 83188
Dusp10
Name: dual specificity phosphatase 10
Synonyms: MKP-5, 2610306G15Rik, MKP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 63953
HGNC: HGNC:3065
Homologene: 5215
Olfr237
Name: olfactory receptor 237
Synonyms: GA_x6K02T2P3E9-4404793-4403861, GA_x6K02T08UK8-1-481, MOR261-4, Olfr438, Olfr237-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258648
Homologene: 88438
Rsph9
Name: radial spoke head 9 homolog (Chlamydomonas)
Synonyms: 1700027N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75564
Homologene: 12606
Adamtsl5
Name: ADAMTS-like 5
Synonyms: 2010109H09Rik, Thsd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66548
Homologene: 19803
Vmn1r158
Name: vomeronasal 1 receptor 158
Synonyms: Gm16455
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043067
Homologene: 104166
Cldn19
Name: claudin 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242653
HGNC: HGNC:2040
Homologene: 17528
Fam181a
Name: family with sequence similarity 181, member A
Synonyms: EG544888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100504156
Homologene: 34701
Vmn1r227
Name: vomeronasal 1 receptor 227
Synonyms: V1re6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171229
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 86,199,847 bp
  • A to G, chromosome 1 at 126,027,724 bp
  • A to G, chromosome 1 at 165,215,768 bp
  • A to G, chromosome 1 at 184,037,013 bp
  • T to A, chromosome 2 at 16,650,215 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • T to A, chromosome 3 at 90,249,395 bp
  • G to T, chromosome 4 at 119,255,733 bp
  • G to A, chromosome 4 at 127,048,152 bp
  • T to C, chromosome 4 at 146,195,171 bp
  • T to G, chromosome 4 at 149,104,303 bp
  • T to C, chromosome 5 at 123,647,883 bp
  • T to C, chromosome 5 at 144,556,231 bp
  • T to C, chromosome 6 at 43,154,027 bp
  • C to T, chromosome 6 at 90,107,483 bp
  • A to T, chromosome 6 at 97,201,935 bp
  • A to G, chromosome 6 at 125,163,062 bp
  • T to A, chromosome 7 at 22,790,374 bp
  • T to C, chromosome 7 at 29,528,299 bp
  • G to T, chromosome 7 at 55,892,031 bp
  • T to A, chromosome 7 at 64,268,954 bp
  • T to C, chromosome 7 at 89,976,965 bp
  • A to C, chromosome 9 at 37,416,879 bp
  • A to T, chromosome 9 at 48,937,242 bp
  • T to A, chromosome 9 at 107,907,574 bp
  • T to G, chromosome 10 at 20,332,384 bp
  • G to T, chromosome 10 at 53,629,997 bp
  • C to T, chromosome 10 at 80,345,358 bp
  • A to T, chromosome 10 at 107,768,973 bp
  • T to C, chromosome 11 at 35,875,896 bp
  • G to A, chromosome 11 at 48,908,217 bp
  • T to C, chromosome 11 at 83,013,388 bp
  • A to G, chromosome 11 at 102,284,994 bp
  • G to A, chromosome 11 at 116,534,804 bp
  • C to A, chromosome 12 at 25,003,010 bp
  • T to A, chromosome 12 at 70,163,368 bp
  • C to T, chromosome 12 at 103,316,133 bp
  • A to G, chromosome 12 at 110,809,978 bp
  • A to C, chromosome 15 at 58,108,337 bp
  • A to G, chromosome 16 at 18,219,174 bp
  • G to A, chromosome 17 at 20,735,390 bp
  • G to A, chromosome 17 at 30,790,917 bp
  • G to T, chromosome 17 at 46,135,082 bp
  • T to C, chromosome 17 at 64,636,507 bp
  • G to T, chromosome 19 at 24,920,630 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5238 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042809-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.