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Strain Name:
C57BL/6J-MtgxR5251Btlr/Mmmh
Stock Number:
042822-MU
Citation ID:
RRID:MMRRC_042822-MU
Other Names:
R5251 (G1), C57BL/6J-MtgxR5251Btlr
Major Collection:

Strain Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70699
Homologene: 45971
Exosc5
Name: exosome component 5
Synonyms: D7Wsu180e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27998
Homologene: 5981
Tmem259
Name: transmembrane protein 259
Synonyms: membralin, ORF61
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216157
Homologene: 14122
Afap1
Name: actin filament associated protein 1
Synonyms: 9630044L16Rik, 2600003E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70292
Homologene: 11009
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268902
Homologene: 43188
Camta1
Name: calmodulin binding transcription activator 1
Synonyms: 2310058O09Rik, 1810059M14Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100072
Homologene: 18942
Fgfr3
Name: fibroblast growth factor receptor 3
Synonyms: HBGFR, Fgfr-3, sam3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14184
HGNC: HGNC:3690
Homologene: 55437
Cdc34
Name: cell division cycle 34
Synonyms: UBE2R1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216150
VEGA: 10
HGNC: HGNC:1734
Homologene: 55815
Cenph
Name: centromere protein H
Synonyms: ENP, CENP-H, 1700021I11Rik, 2610042E16Rik, 2810046K12Rik, 2410018A12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26886
Homologene: 32519
Txndc5
Name: thioredoxin domain containing 5
Synonyms: PC-TRP, ERp46
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105245
Homologene: 11125
Man1a2
Name: mannosidase, alpha, class 1A, member 2
Synonyms: Man1b
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17156
HGNC: HGNC:6822
Homologene: 55982
Dph2
Name: DPH2 homolog
Synonyms: 9130020C19Rik, Dph2l2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67728
HGNC: HGNC:3004
Homologene: 1060
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 260315
Homologene: 56688
Trpc3
Name: transient receptor potential cation channel, subfamily C, member 3
Synonyms: Trp3, Trrp3, Trcp3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22065
Homologene: 20708
Arhgef5
Name: Rho guanine nucleotide exchange factor 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54324
Homologene: 66300
Zbtb38
Name: zinc finger and BTB domain containing 38
Synonyms: A930014K01Rik, Zenon homolog, CIBZ
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245007
Homologene: 19529
Mertk
Name: MER proto-oncogene tyrosine kinase
Synonyms: Tyro 12, Nyk, Eyk, Mer, nmf12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17289
HGNC: HGNC:7027
Homologene: 4626
Nme8
Name: NME/NM23 family member 8
Synonyms: 1700056P15Rik, Sptrx-2, Txndc3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73412
Homologene: 9593
Camk4
Name: calcium/calmodulin-dependent protein kinase IV
Synonyms: Ca2+/calmodulin-dependent protein kinase type IV/Gr, CaMKIV/Gr, CaMKIV, A430110E23Rik, D18Bwg0362e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12326
VEGA: 18
HGNC: HGNC:1464
Homologene: 100780
Ankrd16
Name: ankyrin repeat domain 16
Synonyms: D430029B21Rik, 2810455F06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320816
Homologene: 19509
Colq
Name: collagen like tail subunit of asymmetric acetylcholinesterase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 382864
HGNC: HGNC:2226
Sphkap
Name: SPHK1 interactor, AKAP domain containing
Synonyms: A930009L15Rik, 4930544G21Rik, SKIP
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77629
Homologene: 18172
Hs3st6
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328779
VEGA: 17
Homologene: 84633
Tchhl1
Name: trichohyalin-like 1
Synonyms: S100a17, Thhl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71325
Homologene: 84625
Prl7d1
Name: prolactin family 7, subfamily d, member 1
Synonyms: PRP, PLF-RP, Plfr
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18814
Homologene: 7894
Igkv13-84
Name: immunoglobulin kappa chain variable 13-84
Synonyms: Igk-V33
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692152
Prss23os
Name: serine protease 23, opposite strand
Synonyms: 4930479H08Rik, 1700019G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Sh2d1b2
Name: SH2 domain containing 1B2
Synonyms: Eat2b, Ert, Sh2d1c
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545378
Homologene: 86825
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 83,277,207 bp
  • A to T, chromosome 1 at 170,250,073 bp
  • T to A, chromosome 2 at 11,778,741 bp
  • A to G, chromosome 2 at 26,439,345 bp
  • T to C, chromosome 2 at 128,729,455 bp
  • A to T, chromosome 3 at 36,670,954 bp
  • T to C, chromosome 3 at 93,470,553 bp
  • T to C, chromosome 3 at 100,620,099 bp
  • A to T, chromosome 4 at 117,890,346 bp
  • A to G, chromosome 4 at 123,449,967 bp
  • A to G, chromosome 4 at 151,163,884 bp
  • C to T, chromosome 5 at 33,735,556 bp
  • A to T, chromosome 5 at 35,950,892 bp
  • A to G, chromosome 6 at 35,196,482 bp
  • A to G, chromosome 6 at 43,272,881 bp
  • A to T, chromosome 6 at 68,939,788 bp
  • A to G, chromosome 7 at 25,667,755 bp
  • T to C, chromosome 7 at 89,510,322 bp
  • A to T, chromosome 7 at 126,783,344 bp
  • G to T, chromosome 9 at 96,687,108 bp
  • G to A, chromosome 10 at 60,301,700 bp
  • C to T, chromosome 10 at 79,685,256 bp
  • A to G, chromosome 10 at 79,975,833 bp
  • A to G, chromosome 10 at 109,853,253 bp
  • T to C, chromosome 13 at 19,660,625 bp
  • T to C, chromosome 13 at 27,709,244 bp
  • T to C, chromosome 13 at 38,501,664 bp
  • T to A, chromosome 13 at 100,761,840 bp
  • A to G, chromosome 14 at 31,539,819 bp
  • C to T, chromosome 16 at 73,897,182 bp
  • T to A, chromosome 17 at 24,757,985 bp
  • A to G, chromosome 18 at 33,184,879 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5251 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042822-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.