Strain Name:
C57BL/6J-MtgxR5252Btlr/Mmmh
Stock Number:
042823-MU
Citation ID:
RRID:MMRRC_042823-MU
Other Names:
R5252 (G1), C57BL/6J-MtgxR5252Btlr
Major Collection:

Gene Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Serpinc1
Name: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
Synonyms: At-3, antithrombin, ATIII, At3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 11905
HGNC: HGNC:775
Homologene: 20139
Pkp4
Name: plakophilin 4
Synonyms: p0071, 9430019K17Rik, 5031422I09Rik, Armrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227937
HGNC: HGNC:9026
Homologene: 2689
Utp20
Name: UTP20 small subunit processome component
Synonyms: DRIM, 3830408P06Rik, mDRIM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70683
VEGA: 10
Homologene: 38373
Cdk7
Name: cyclin-dependent kinase 7
Synonyms: Crk4, CRK4 PK (CDC2-related-kinase-4 protein kinase), Cdkn7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 12572
VEGA: 13
HGNC: HGNC:1778
Homologene: 1363
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 27223
Homologene: 4137
Gpr139
Name: G protein-coupled receptor 139
Synonyms: GPRg1, LOC209776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 209776
Homologene: 45860
Alox12b
Name: arachidonate 12-lipoxygenase, 12R type
Synonyms: Aloxe2, e-LOX2, 12R-LOX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11686
HGNC: HGNC:430
Homologene: 884
Ppard
Name: peroxisome proliferator activator receptor delta
Synonyms: Pparb, PPAR-delta, NUC1, Pparb/d, Peroxisome proliferator-activated receptor beta, Nr1c2, PPARdelta/beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 19015
HGNC: HGNC:9235
Homologene: 4544
Sptlc2
Name: serine palmitoyltransferase, long chain base subunit 2
Synonyms: Spt2, LCB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20773
Homologene: 21610
Rabgap1
Name: RAB GTPase activating protein 1
Synonyms: Gapcena
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227800
Homologene: 49301
Cux1
Name: cut-like homeobox 1
Synonyms: Cux-1, Cux, Cutl1, CDP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 13047
HGNC: HGNC:2557
Evi5
Name: ecotropic viral integration site 5
Synonyms: NB4S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14020
HGNC: HGNC:3501
Homologene: 121902
Fas
Name: Fas (TNF receptor superfamily member 6)
Synonyms: CD95, Tnfrsf6, APO-1, TNFR6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 14102
VEGA: 19
Homologene: 27
Lin54
Name: lin-54 homolog (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231506
Homologene: 18212
Ccdc141
Name: coiled-coil domain containing 141
Synonyms: 2610301F02Rik, CAMDI, ENSMUSG00000075261
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 545428
Homologene: 52149
Ms4a6d
Name: membrane-spanning 4-domains, subfamily A, member 6D
Synonyms: Ms4a11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 68774
Homologene: 130755
Canx
Name: calnexin
Synonyms: 1110069N15Rik, CNX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12330
HGNC: HGNC:1473
Homologene: 1324
Cdk12
Name: cyclin-dependent kinase 12
Synonyms: D11Ertd752e, 1810022J16Rik, Crk7, Crkrs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69131
Homologene: 128632
Epn2
Name: epsin 2
Synonyms: Ibp2, 9530051D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 13855
Homologene: 40710
Cxxc1
Name: CXXC finger 1 (PHD domain)
Synonyms: Cgbp, 5830420C16Rik, 2410002I16Rik, PHF18, Cfp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 74322
VEGA: 18
Homologene: 32221
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: A630028O16Rik, LOC380767, 9330197E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 211978
VEGA: 12
Homologene: 9102
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: USH1D, mdfw, ahl, nmf112, nmf181, nmf252, 4930542A03Rik, sals, bob
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22295
Homologene: 11142
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: 2900022L05Rik, Dnahc2, D330014H01Rik, Dnhd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1289Clo, b2b1727Clo, b2b598Clo, lrd, b2b1203Clo, b2b1279Clo, avc4, Dnahc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Ush2a
Name: usherin
Synonyms: Ush2a, Ushrn, MUSH2A, A930037M10Rik, A930011D15Rik, LOC269160, LOC381317
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22283
Homologene: 66151
Slc5a8
Name: solute carrier family 5 (iodide transporter), member 8
Synonyms: SMCT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216225
VEGA: 10
Homologene: 64832
1700022I11Rik
Name: RIKEN cDNA 1700022I11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 67317
Homologene: 52406
Dll1
Name: delta like canonical Notch ligand 1
Synonyms: Delta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13388
HGNC: HGNC:2908
Homologene: 4104
Frmd4a
Name: FERM domain containing 4A
Synonyms: 2700017I06Rik, C230040M21Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 209630
Homologene: 9971
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: Prkwnk4, 2010002J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69847
Homologene: 13020
Unc13a
Name: unc-13 homolog A
Synonyms: Munc13-1, 2410078G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382018
Homologene: 11279
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 665033
Homologene: 122792
Olfr967
Name: olfactory receptor 967
Synonyms: MOR171-30P, GA_x6K02T2PVTD-33447884-33448816
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258086
VEGA: 9
HGNC: HGNC:8484
Homologene: 115535
Tent5c
Name: terminal nucleotidyltransferase 5C
Synonyms: Fam46c, 4930431B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 74645
Homologene: 56783
Olfr1217
Name: olfactory receptor 1217
Synonyms: MOR233-4, GA_x6K02T2Q125-50504545-50503631
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258903
Homologene: 73985
Ano8
Name: anoctamin 8
Synonyms: Tmem16h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382014
Homologene: 124473
Pilrb1
Name: paired immunoglobin-like type 2 receptor beta 1
Synonyms: Pilrb, Fdact
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 170741
Homologene: 114502
Inpp1
Name: inositol polyphosphate-1-phosphatase
Synonyms: 2300002C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16329
HGNC: HGNC:6071
Homologene: 1655
Nexn
Name: nexilin
Synonyms: 1110046H09Rik, nF actin binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 68810
Homologene: 44892
Slco4a1
Name: solute carrier organic anion transporter family, member 4a1
Synonyms: Slc21a12, OATP-E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 108115
Homologene: 9482
H2-Q4
Name: histocompatibility 2, Q region locus 4
Synonyms: H-2Q4, H2-Gs10, Qa-4, Qb-1, Qa4, Qb1, Qat-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 15015
HGNC: HGNC:4931
Homologene: 128352
Gm12034
Name: predicted gene 12034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 100502908
Catspere1
Name: cation channel sperm associated auxiliary subunit epsilon 1
Synonyms: Gm7068, Catspere
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 631584
Homologene: 52161
Cabp4
Name: calcium binding protein 4
Synonyms: 2410038D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 73660
VEGA: 19
HGNC: HGNC:1386
Homologene: 16927
Ighv9-2
Name: immunoglobulin heavy variable V9-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 629818
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,350,411 bp
  • T to G, chromosome 1 at 52,794,547 bp
  • T to C, chromosome 1 at 160,989,621 bp
  • A to C, chromosome 1 at 177,872,115 bp
  • A to T, chromosome 1 at 188,821,717 bp
  • T to C, chromosome 2 at 4,537,307 bp
  • T to C, chromosome 2 at 37,475,357 bp
  • G to T, chromosome 2 at 59,344,895 bp
  • A to G, chromosome 2 at 77,132,249 bp
  • A to T, chromosome 2 at 89,023,254 bp
  • A to G, chromosome 2 at 121,243,983 bp
  • G to A, chromosome 2 at 180,464,459 bp
  • A to G, chromosome 3 at 100,472,708 bp
  • T to C, chromosome 3 at 152,237,953 bp
  • T to A, chromosome 4 at 42,971,706 bp
  • C to T, chromosome 5 at 100,480,204 bp
  • T to C, chromosome 5 at 107,795,752 bp
  • T to C, chromosome 5 at 136,308,297 bp
  • T to A, chromosome 5 at 137,855,053 bp
  • T to C, chromosome 6 at 84,186,468 bp
  • A to G, chromosome 7 at 119,145,204 bp
  • A to T, chromosome 8 at 71,482,617 bp
  • T to A, chromosome 8 at 71,652,564 bp
  • T to G, chromosome 9 at 39,750,488 bp
  • T to C, chromosome 9 at 105,940,290 bp
  • T to C, chromosome 10 at 60,304,481 bp
  • A to T, chromosome 10 at 88,750,670 bp
  • T to C, chromosome 10 at 88,906,347 bp
  • A to G, chromosome 11 at 20,446,629 bp
  • A to T, chromosome 11 at 50,308,794 bp
  • A to T, chromosome 11 at 61,534,551 bp
  • G to T, chromosome 11 at 69,165,936 bp
  • A to G, chromosome 11 at 69,529,469 bp
  • A to T, chromosome 11 at 98,243,509 bp
  • A to G, chromosome 11 at 101,268,748 bp
  • A to G, chromosome 12 at 79,268,982 bp
  • A to T, chromosome 12 at 87,336,055 bp
  • A to G, chromosome 12 at 114,109,218 bp
  • A to G, chromosome 12 at 118,125,941 bp
  • T to A, chromosome 13 at 100,730,460 bp
  • T to C, chromosome 17 at 15,368,689 bp
  • G to A, chromosome 17 at 28,298,848 bp
  • T to C, chromosome 17 at 35,380,435 bp
  • C to T, chromosome 18 at 74,219,951 bp
  • T to C, chromosome 19 at 4,136,068 bp
  • T to G, chromosome 19 at 11,592,947 bp
  • T to C, chromosome 19 at 34,316,643 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5252 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042823-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.