Strain Name:
C57BL/6J-MtgxR5264Btlr/Mmmh
Stock Number:
042832-MU
Citation ID:
RRID:MMRRC_042832-MU
Other Names:
R5264 (G1), C57BL/6J-MtgxR5264Btlr
Major Collection:

Gene Information

Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20349
Homologene: 8247
Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22419
Homologene: 22530
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Ptprk
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 18193
VEGA: 13
Homologene: 32543
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: 9330175N20Rik, RPC1, RPC155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218832
VEGA: 14
Homologene: 5124
Polr3b
Name: polymerase (RNA) III (DNA directed) polypeptide B
Synonyms: 2700078H01Rik, A330032P03Rik, RPC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70428
VEGA: 10
Homologene: 6981
Plcg2
Name: phospholipase C, gamma 2
Synonyms: PLCgamma2, Plcg-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234779
HGNC: HGNC:9066
Homologene: 55671
Ckap2l
Name: cytoskeleton associated protein 2-like
Synonyms: Radmis, 2610318C08Rik, 2010016H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70466
Homologene: 51866
Zfp617
Name: zinc finger protein 617
Synonyms: Zfps11-6, Zinc finger protein s11-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 170938
Homologene: 135689
Ttll4
Name: tubulin tyrosine ligase-like family, member 4
Synonyms: 4632407P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67534
Homologene: 8764
Zranb1
Name: zinc finger, RAN-binding domain containing 1
Synonyms: 9330160G10Rik, D7Wsu87e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 360216
Homologene: 9728
Adamtsl1
Name: ADAMTS-like 1
Synonyms: 6720426B09Rik, 5930437A14Rik, punctin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 77739
Homologene: 64642
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 329002
Homologene: 7198
Phactr4
Name: phosphatase and actin regulator 4
Synonyms: C330013F19Rik, 3110001B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100169
Homologene: 41537
Samd12
Name: sterile alpha motif domain containing 12
Synonyms: A830094I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 320679
Homologene: 35979
Ptgs2
Name: prostaglandin-endoperoxide synthase 2
Synonyms: PGHS-2, cyclooxygenase-2, Pghs2, PHS-2, COX2, Tis10, prostaglandin G/H synthase, cyclooxygenase 2, Cox-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19225
HGNC: HGNC:9605
Homologene: 31000
Col4a4
Name: collagen, type IV, alpha 4
Synonyms: E130010M05Rik, [a]4(IV)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12829
HGNC: HGNC:2206
Homologene: 20071
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 545370
Homologene: 23741
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 26875
Homologene: 69111
Casc1
Name: cancer susceptibility candidate 1
Synonyms: Las1, A230084G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320662
Homologene: 41242
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: sucrase-isomaltase, 2010204N08Rik, Si-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 69983
Homologene: 37424
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230500
Homologene: 19952
Tcp11l2
Name: t-complex 11 (mouse) like 2
Synonyms: E430026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216198
Homologene: 17129
Psd3
Name: pleckstrin and Sec7 domain containing 3
Synonyms: 4931420C21Rik, EFA6D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234353
Homologene: 87257
Morc2b
Name: microrchidia 2B
Synonyms: 4932411A10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240069
Homologene: 18615
Wdr66
Name: WD repeat domain 66
Synonyms: 4930415N18Rik, 4933428F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269701
Homologene: 16964
Gns
Name: glucosamine (N-acetyl)-6-sulfatase
Synonyms: G6S, 2610016K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 75612
VEGA: 10
HGNC: HGNC:4422
Homologene: 1568
Rsph4a
Name: radial spoke head 4 homolog A (Chlamydomonas)
Synonyms: Rshl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 212892
Homologene: 71779
Cacnb2
Name: calcium channel, voltage-dependent, beta 2 subunit
Synonyms: Cchb2, Cavbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12296
HGNC: HGNC:1402
Homologene: 75191
Stat2
Name: signal transducer and activator of transcription 2
Synonyms: 1600010G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 20847
VEGA: 10
Homologene: 3952
Pex16
Name: peroxisomal biogenesis factor 16
Synonyms: peroxisome biogenesis factor 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18633
HGNC: HGNC:8857
Homologene: 3537
Olfr33
Name: olfactory receptor 33
Synonyms: GA_x6K02T2PBJ9-5431102-5430146, MTPCR33, MOR11-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18332
Homologene: 81534
Gcc2
Name: GRIP and coiled-coil domain containing 2
Synonyms: 0610043A03Rik, 2210420P05Rik, 2600014C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 70297
Homologene: 45639
Paqr8
Name: progestin and adipoQ receptor family member VIII
Synonyms: 1700019B16Rik, 3110001D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74229
Homologene: 32702
Elovl4
Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 83603
Homologene: 41488
Spag6l
Name: sperm associated antigen 6-like
Synonyms: PF16, Spag6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 50525
Homologene: 8252
Fbln5
Name: fibulin 5
Synonyms: EVEC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 23876
VEGA: 12
HGNC: HGNC:3602
Homologene: 38170
Spaca1
Name: sperm acrosome associated 1
Synonyms: 1700124L11Rik, 4930540L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 67652
Homologene: 12170
Vmn2r67
Name: vomeronasal 2, receptor 67
Synonyms: EG620672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 620672
Homologene: 115466
Mrgprb5
Name: MAS-related GPR, member B5
Synonyms: MrgB5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 404239
Homologene: 115575
Fank1
Name: fibronectin type 3 and ankyrin repeat domains 1
Synonyms: 1700007B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66930
Homologene: 12055
Lrrc8b
Name: leucine rich repeat containing 8 family, member B
Synonyms: 2210408K08Rik, R75581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 433926
Homologene: 9103
R3hdm4
Name: R3H domain containing 4
Synonyms: C030046I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 109284
VEGA: 10
Homologene: 16343
Socs5
Name: suppressor of cytokine signaling 5
Synonyms: 1810018L08Rik, Cish5, SOCS-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 56468
VEGA: 17
Homologene: 10495
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 100101919
Homologene: 41287
Dcun1d4
Name: DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100737
Homologene: 22867
Smoc1
Name: SPARC related modular calcium binding 1
Synonyms: SRG, SPARC-related protein, 2600002F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 64075
Homologene: 56943
Fbxl21
Name: F-box and leucine-rich repeat protein 21
Synonyms: FBL3B, FBXL3B, Psttm, D630045D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 213311
Homologene: 78724
Nectin4
Name: nectin cell adhesion molecule 4
Synonyms: 1200017F15Rik, Pvrl4, nectin 4, Prr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71740
Homologene: 32744
Ppp1r35
Name: protein phosphatase 1, regulatory subunit 35
Synonyms: 2010007H12Rik, 2010011D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69871
Homologene: 12337
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,935,108 bp
  • T to G, chromosome 1 at 46,659,431 bp
  • A to G, chromosome 1 at 74,686,376 bp
  • C to T, chromosome 1 at 82,493,591 bp
  • A to G, chromosome 1 at 150,102,730 bp
  • A to G, chromosome 1 at 150,679,514 bp
  • A to T, chromosome 1 at 171,383,705 bp
  • T to C, chromosome 1 at 189,832,800 bp
  • A to G, chromosome 2 at 14,982,865 bp
  • A to G, chromosome 2 at 18,745,513 bp
  • A to G, chromosome 2 at 92,374,743 bp
  • A to T, chromosome 2 at 129,285,379 bp
  • A to G, chromosome 3 at 72,949,756 bp
  • C to A, chromosome 4 at 34,049,863 bp
  • A to G, chromosome 4 at 86,343,144 bp
  • G to A, chromosome 4 at 99,878,170 bp
  • T to C, chromosome 4 at 132,370,982 bp
  • A to C, chromosome 5 at 14,226,648 bp
  • G to A, chromosome 5 at 14,676,923 bp
  • A to G, chromosome 5 at 73,555,424 bp
  • A to T, chromosome 5 at 105,480,252 bp
  • A to G, chromosome 5 at 123,297,577 bp
  • G to A, chromosome 5 at 137,780,024 bp
  • A to T, chromosome 6 at 119,433,852 bp
  • A to G, chromosome 6 at 145,181,776 bp
  • G to A, chromosome 7 at 48,168,048 bp
  • T to C, chromosome 7 at 85,152,245 bp
  • T to C, chromosome 7 at 102,714,351 bp
  • CTGATGATGATG to CTGATGATGATGATG, chromosome 7 at 132,982,827 bp
  • A to G, chromosome 7 at 133,879,892 bp
  • A to T, chromosome 8 at 67,713,725 bp
  • A to G, chromosome 8 at 71,933,041 bp
  • A to T, chromosome 8 at 117,634,793 bp
  • T to C, chromosome 9 at 83,780,764 bp
  • A to G, chromosome 10 at 28,585,586 bp
  • A to G, chromosome 10 at 33,909,383 bp
  • T to A, chromosome 10 at 58,304,116 bp
  • A to G, chromosome 10 at 79,913,341 bp
  • A to G, chromosome 10 at 84,613,660 bp
  • A to T, chromosome 10 at 84,667,416 bp
  • A to G, chromosome 10 at 121,380,185 bp
  • T to A, chromosome 10 at 128,281,065 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • T to A, chromosome 12 at 81,104,700 bp
  • T to A, chromosome 12 at 101,757,444 bp
  • C to T, chromosome 13 at 55,247,346 bp
  • T to C, chromosome 13 at 56,532,323 bp
  • T to C, chromosome 14 at 24,454,941 bp
  • C to T, chromosome 15 at 53,860,273 bp
  • T to C, chromosome 17 at 33,138,379 bp
  • T to A, chromosome 17 at 87,134,341 bp
  • T to C, chromosome 18 at 82,630,094 bp
  • T to C, chromosome 18 at 82,658,073 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5264 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042832-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.