Strain Name:
C57BL/6J-MtgxR5336Btlr/Mmmh
Stock Number:
042844-MU
Citation ID:
RRID:MMRRC_042844-MU
Other Names:
R5336 (G1), C57BL/6J-MtgxR5336Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Ptprf
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Anapc5
Name: anaphase-promoting complex subunit 5
Synonyms: 2510006G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 59008
Homologene: 41118
Ints3
Name: integrator complex subunit 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229543
Homologene: 11309
Ctnnd1
Name: catenin (cadherin associated protein), delta 1
Synonyms: P120, Ctnnd, p120-catenin, Catns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12388
HGNC: HGNC:2515
Homologene: 1017
Slf1
Name: SMC5-SMC6 complex localization factor 1
Synonyms: C730024G01Rik, 2700017A04Rik, Brctx, Brctd1, Ankrd32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105377
Homologene: 13000
Otof
Name: otoferlin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Eef1a1
Name: eukaryotic translation elongation factor 1 alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13627
HGNC: HGNC:3189
Homologene: 100799
Spag9
Name: sperm associated antigen 9
Synonyms: 4733401I23Rik, syd1, 4831406C20Rik, Mapk8ip4, JIP4, 3110018C07Rik, JLP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70834
Homologene: 2954
Chpf
Name: chondroitin polymerizing factor
Synonyms: 1700028N03Rik, D1Bwg1363e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74241
Homologene: 11574
Mtpn
Name: myotrophin
Synonyms: V1, Gcdp, 5033418D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14489
Homologene: 40607
Ddost
Name: dolichyl-di-phosphooligosaccharide-protein glycotransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13200
HGNC: HGNC:2728
Homologene: 3821
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, SEC3, A730011E05Rik, 2810407P21Rik, Sec3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69940
Homologene: 41241
Zfp874a
Name: zinc finger protein 874a
Synonyms: Rslcan15, C330011K17Rik, Zfp874
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238692
Homologene: 135597
H2-T23
Name: histocompatibility 2, T region locus 23
Synonyms: H-2T23, T18c(37), T18c, 37c, 37b, T23d, T23b, Qed-1, Qa1, Qa-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15040
Homologene: 134018
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Col5a3
Name: collagen, type V, alpha 3
Synonyms: Pro-alpha3(V)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53867
Homologene: 9253
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244484
Homologene: 12460
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: PYPAF2, E330007A02Rik, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232827
Homologene: 56789
Eif2ak2
Name: eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms: eIF-2 alpha, Pkr, dsRNA-activated kinase, IFN- type I-induced and dsRNA-activated kinase, IFN-induced and double-stranded RNA-activated kinase, Prkr, Tik, eIF-2 alpha, 4732414G15Rik, 2310047A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19106
HGNC: HGNC:9437
Homologene: 48134
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 1110004O12Rik, 9130019P20Rik, 4933432B13Rik, A530027J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108857
Homologene: 87006
Scara3
Name: scavenger receptor class A, member 3
Synonyms: C130058N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219151
VEGA: 14
Homologene: 9437
Vmn2r85
Name: vomeronasal 2, receptor 85
Synonyms: EG623734
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 623734
Homologene: 129606
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: 6530405F15Rik, CMF608, Adlican2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242050
Homologene: 18712
Cntn4
Name: contactin 4
Synonyms: BIG-2A, Axcam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269784
HGNC: HGNC:2174
Homologene: 14257
Myh4
Name: myosin, heavy polypeptide 4, skeletal muscle
Synonyms: MyHC-IIb, MHC2B, Myhsf, MM, MYH-2B, Minmus, Minimsc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17884
HGNC: HGNC:7574
Homologene: 123880
Tarm1
Name: T cell-interacting, activating receptor on myeloid cells 1
Synonyms: 9930022N03Rik, Gm9904
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 245126
Homologene: 120241
Cav1
Name: caveolin 1, caveolae protein
Synonyms: Cav-1, caveolin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12389
HGNC: HGNC:1527
Homologene: 1330
Sntb1
Name: syntrophin, basic 1
Synonyms: beta1-Syntrophin, 59-1 DAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20649
Homologene: 9618
Trim50
Name: tripartite motif-containing 50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 215061
Homologene: 14548
9330159F19Rik
Name: RIKEN cDNA 9330159F19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212448
VEGA: 10
Homologene: 8804
Gad1-ps
Name: glutamate decarboxylase 1, pseudogene
Synonyms: Gad-1ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382407
VEGA: 10
Tlr1
Name: toll-like receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21897
Homologene: 20694
Ppig
Name: peptidyl-prolyl isomerase G (cyclophilin G)
Synonyms: SRCyp, B230312B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228005
Homologene: 3520
Or1a1
Name: olfactory receptor family 1 subfamily A member 1
Synonyms: GA_x6K02T2P1NL-4348188-4349129, MOR125-5_p, IA7, Olfr403
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 404316
Homologene: 8219
Acyp1
Name: acylphosphatase 1, erythrocyte (common) type
Synonyms: 1110039O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66204
HGNC: HGNC:179
Homologene: 11932
Clec2f
Name: C-type lectin domain family 2, member f
Synonyms: Clrc, Clrz, Clec2f-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435921
Homologene: 136171
AU015228
Name: expressed sequence AU015228
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99169
Ighv9-2
Name: immunoglobulin heavy variable V9-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629818
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 75,475,707 bp
  • T to C, chromosome 2 at 69,750,224 bp
  • C to T, chromosome 2 at 84,616,789 bp
  • T to A, chromosome 2 at 130,101,654 bp
  • T to C, chromosome 3 at 59,320,132 bp
  • G to A, chromosome 3 at 90,396,384 bp
  • A to G, chromosome 4 at 118,235,634 bp
  • T to C, chromosome 4 at 138,309,430 bp
  • T to C, chromosome 5 at 30,376,720 bp
  • A to T, chromosome 5 at 64,925,802 bp
  • T to A, chromosome 5 at 76,535,709 bp
  • T to C, chromosome 5 at 122,807,337 bp
  • C to T, chromosome 5 at 135,367,476 bp
  • T to C, chromosome 6 at 17,317,904 bp
  • C to T, chromosome 6 at 35,512,290 bp
  • T to C, chromosome 6 at 106,662,634 bp
  • T to A, chromosome 6 at 129,019,314 bp
  • T to C, chromosome 7 at 3,497,568 bp
  • T to C, chromosome 7 at 5,328,119 bp
  • T to A, chromosome 8 at 54,632,318 bp
  • A to T, chromosome 9 at 20,799,301 bp
  • G to A, chromosome 9 at 78,480,397 bp
  • A to T, chromosome 10 at 29,224,499 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • T to C, chromosome 10 at 130,422,705 bp
  • A to G, chromosome 11 at 67,259,191 bp
  • T to C, chromosome 11 at 74,196,033 bp
  • A to G, chromosome 11 at 94,043,592 bp
  • A to T, chromosome 12 at 85,280,011 bp
  • A to C, chromosome 12 at 114,109,285 bp
  • T to C, chromosome 13 at 67,442,680 bp
  • A to T, chromosome 13 at 77,106,010 bp
  • A to G, chromosome 14 at 65,931,038 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • C to T, chromosome 15 at 102,111,229 bp
  • T to C, chromosome 17 at 25,392,231 bp
  • T to A, chromosome 17 at 36,031,658 bp
  • A to G, chromosome 17 at 78,874,043 bp
  • T to A, chromosome 18 at 36,646,716 bp
  • A to G, chromosome 19 at 14,454,739 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5336 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042844-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.