Strain Name:
C57BL/6J-MtgxR5431Btlr/Mmmh
Stock Number:
042847-MU
Citation ID:
RRID:MMRRC_042847-MU
Other Names:
R5431 (G1), C57BL/6J-MtgxR5431Btlr
Major Collection:

Gene Information

Cds2
Name: CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Synonyms: 5730460C18Rik, D2Wsu127e, 5730450N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 110911
HGNC: HGNC:1801
Homologene: 37854
Lhx3
Name: LIM homeobox protein 3
Synonyms: P-LIM, mLim-3, Lim3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16871
HGNC: HGNC:6595
Homologene: 7814
Ep400
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, mDomino, p400
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 75560
Homologene: 38779
Gpr151
Name: G protein-coupled receptor 151
Synonyms: GalRL, PGR7, C130082O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 240239
VEGA: 18
Homologene: 18754
Frmd5
Name: FERM domain containing 5
Synonyms: A930004K21Rik, 1500032A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228564
Homologene: 18206
Micu1
Name: mitochondrial calcium uptake 1
Synonyms: Cbara1, C730016L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216001
HGNC: HGNC:1530
Homologene: 4431
Rfx1
Name: regulatory factor X, 1 (influences HLA class II expression)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 19724
HGNC: HGNC:9982
Homologene: 2189
Zc3h14
Name: zinc finger CCCH type containing 14
Synonyms: 1700016A15Rik, 2700069A02Rik, 1010001P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 75553
VEGA: 12
Homologene: 32605
Hdac4
Name: histone deacetylase 4
Synonyms: 4932408F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 208727
Homologene: 55946
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, 9230115F04Rik, Zcchc11, Tent3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230594
Homologene: 35279
Dcaf13
Name: DDB1 and CUL4 associated factor 13
Synonyms: Wdsof1, LOC223499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223499
Homologene: 6430
Gm14393
Name: predicted gene 14393
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 664987
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: Tg(SMN2)89Ahmb, SMN2, 6330570A01Rik, mGlu7a receptor, E130018M02Rik, Gpr1g, mGluR7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Syt2
Name: synaptotagmin II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20980
Homologene: 22516
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, Odz3, 2610100B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23965
Homologene: 22673
Ice1
Name: interactor of little elongation complex ELL subunit 1
Synonyms: BC018507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218333
VEGA: 13
Homologene: 18902
Kel
Name: Kell blood group
Synonyms: CD238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 23925
HGNC: HGNC:6308
Homologene: 362
Elfn1
Name: leucine rich repeat and fibronectin type III, extracellular 1
Synonyms: A930017N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243312
Homologene: 18465
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: skrr, Ryr, calcium release channel isoform 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20190
Homologene: 68069
Gpr152
Name: G protein-coupled receptor 152
Synonyms: A930009H15Rik, LOC269053
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 269053
VEGA: 19
Homologene: 35474
Fgfr4
Name: fibroblast growth factor receptor 4
Synonyms: Fgfr-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 14186
HGNC: HGNC:3691
Homologene: 20461
Ciita
Name: class II transactivator
Synonyms: C2ta, Gm9475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12265
VEGA: 16
HGNC: HGNC:7067
Homologene: 207
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Myt1l
Name: myelin transcription factor 1-like
Synonyms: 2900093J19Rik, Pmng1, Nztf1, Png-1, 2900046C06Rik, C630034G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 17933
VEGA: 12
HGNC: HGNC:7623
Homologene: 7435
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75823
Homologene: 27384
Cerkl
Name: ceramide kinase-like
Synonyms: Rp26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228094
Homologene: 52396
Sgcz
Name: sarcoglycan zeta
Synonyms: C230085N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 244431
Homologene: 26726
Ggt6
Name: gamma-glutamyltransferase 6
Synonyms: 9030405D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 71522
Homologene: 51918
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381293
Homologene: 8916
Flnc
Name: filamin C, gamma
Synonyms: actin binding protein 280, Fln2, 1110055E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Tcaf3
Name: TRPM8 channel-associated factor 3
Synonyms: Fam115e, Eapa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 403088
Homologene: 74374
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Sntb1
Name: syntrophin, basic 1
Synonyms: 59-1 DAP, beta1-Syntrophin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 20649
Homologene: 9618
B4galnt3
Name: beta-1,4-N-acetyl-galactosaminyl transferase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 330406
Homologene: 18328
2310061N02Rik
Name: RIKEN cDNA 2310061N02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 69661
Homologene: 130759
Wrap73
Name: WD repeat containing, antisense to Trp73
Synonyms: 2610044M17Rik, DD57, 5330425N03Rik, Wdr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 59002
Homologene: 9857
Eno3
Name: enolase 3, beta muscle
Synonyms: Eno-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 13808
HGNC: HGNC:3354
Homologene: 68215
Gad1-ps
Name: glutamate decarboxylase 1, pseudogene
Synonyms: Gad-1ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 382407
VEGA: 10
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 627569
Homologene: 129606
Fam178b
Name: family with sequence similarity 178, member B
Synonyms: 1700024G10Rik, LOC381337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381337
Homologene: 87288
4921539E11Rik
Name: RIKEN cDNA 4921539E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 70941
Homologene: 52843
Tgfbr2
Name: transforming growth factor, beta receptor II
Synonyms: TbetaRII, TBR-II, 1110020H15Rik, TbetaR-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 21813
VEGA: 9
Homologene: 2435
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 78895
Homologene: 12842
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 104816
VEGA: 12
Homologene: 113390
Dnal4
Name: dynein, axonemal, light chain 4
Synonyms: Dnalc4, D15Ertd424e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 54152
HGNC: HGNC:2955
Homologene: 38094
Fam92b
Name: family with sequence similarity 92, member B
Synonyms: 1700120B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 436062
Homologene: 78513
Bmp5
Name: bone morphogenetic protein 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12160
HGNC: HGNC:1072
Homologene: 22412
BC035044
Name: cDNA sequence BC035044
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232406
Rnase2a
Name: ribonuclease, RNase A family, 2A (liver, eosinophil-derived neurotoxin)
Synonyms: Ear11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 93726
VEGA: 14
Homologene: 121614
C330018D20Rik
Name: RIKEN cDNA C330018D20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 77422
Homologene: 35412
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 20,117,836 bp
  • T to C, chromosome 1 at 36,632,485 bp
  • T to A, chromosome 1 at 91,972,790 bp
  • A to G, chromosome 1 at 134,740,957 bp
  • T to C, chromosome 1 at 136,496,695 bp
  • T to C, chromosome 2 at 26,201,118 bp
  • C to T, chromosome 2 at 79,341,335 bp
  • T to C, chromosome 2 at 121,562,909 bp
  • C to A, chromosome 2 at 126,126,931 bp
  • T to A, chromosome 2 at 132,302,170 bp
  • G to A, chromosome 2 at 175,063,876 bp
  • C to T, chromosome 4 at 15,986,593 bp
  • T to C, chromosome 4 at 103,270,848 bp
  • T to A, chromosome 4 at 108,491,412 bp
  • G to A, chromosome 4 at 154,145,274 bp
  • A to G, chromosome 5 at 109,091,818 bp
  • A to G, chromosome 5 at 110,676,554 bp
  • A to G, chromosome 5 at 139,971,568 bp
  • A to G, chromosome 6 at 29,456,384 bp
  • G to A, chromosome 6 at 41,698,420 bp
  • A to G, chromosome 6 at 42,597,185 bp
  • G to A, chromosome 6 at 111,358,426 bp
  • T to A, chromosome 6 at 120,218,967 bp
  • A to G, chromosome 6 at 128,885,007 bp
  • T to A, chromosome 7 at 29,109,812 bp
  • T to A, chromosome 8 at 37,639,984 bp
  • C to A, chromosome 8 at 48,367,377 bp
  • C to T, chromosome 8 at 84,082,720 bp
  • C to A, chromosome 8 at 120,167,303 bp
  • C to T, chromosome 9 at 75,893,709 bp
  • T to C, chromosome 9 at 109,100,772 bp
  • G to T, chromosome 9 at 116,131,601 bp
  • T to C, chromosome 10 at 59,750,521 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • C to T, chromosome 11 at 70,660,800 bp
  • A to G, chromosome 11 at 72,437,738 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • A to G, chromosome 12 at 98,780,065 bp
  • T to A, chromosome 12 at 112,123,412 bp
  • G to A, chromosome 13 at 55,156,651 bp
  • A to G, chromosome 13 at 70,592,650 bp
  • T to C, chromosome 14 at 51,255,563 bp
  • C to A, chromosome 15 at 39,123,224 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • C to T, chromosome 15 at 79,762,447 bp
  • T to C, chromosome 15 at 94,529,486 bp
  • C to T, chromosome 16 at 10,523,792 bp
  • T to C, chromosome 16 at 88,707,538 bp
  • A to C, chromosome 18 at 42,578,867 bp
  • A to T, chromosome 18 at 56,957,856 bp
  • T to A, chromosome 19 at 4,143,747 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5431 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042847-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.