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Strain Name:
C57BL/6J-MtgxR5431Btlr/Mmmh
Stock Number:
042847-MU
Citation ID:
RRID:MMRRC_042847-MU
Other Names:
R5431 (G1), C57BL/6J-MtgxR5431Btlr
Major Collection:

Strain Information

Cds2
Name: CDP-diacylglycerol synthase 2
Synonyms: 5730460C18Rik, D2Wsu127e, 5730450N06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110911
HGNC: HGNC:1801
Homologene: 37854
Lhx3
Name: LIM homeobox protein 3
Synonyms: P-LIM, mLim-3, Lim3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16871
HGNC: HGNC:6595
Homologene: 7814
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Gpr151
Name: G protein-coupled receptor 151
Synonyms: C130082O03Rik, GalRL, PGR7
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240239
VEGA: 18
Homologene: 18754
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Micu1
Name: mitochondrial calcium uptake 1
Synonyms: C730016L05Rik, Cbara1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216001
HGNC: HGNC:1530
Homologene: 4431
Rfx1
Name: regulatory factor X, 1 (influences HLA class II expression)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19724
HGNC: HGNC:9982
Homologene: 2189
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 20,117,836 bp
  • T to C, chromosome 1 at 36,632,485 bp
  • T to A, chromosome 1 at 91,972,790 bp
  • A to G, chromosome 1 at 134,740,957 bp
  • T to C, chromosome 1 at 136,496,695 bp
  • T to C, chromosome 2 at 26,201,118 bp
  • C to T, chromosome 2 at 79,341,335 bp
  • T to C, chromosome 2 at 121,562,909 bp
  • C to A, chromosome 2 at 126,126,931 bp
  • T to A, chromosome 2 at 132,302,170 bp
  • G to A, chromosome 2 at 175,063,876 bp
  • C to T, chromosome 4 at 15,986,593 bp
  • T to C, chromosome 4 at 103,270,848 bp
  • T to A, chromosome 4 at 108,491,412 bp
  • G to A, chromosome 4 at 154,145,274 bp
  • A to G, chromosome 5 at 109,091,818 bp
  • A to G, chromosome 5 at 110,676,554 bp
  • A to G, chromosome 5 at 139,971,568 bp
  • A to G, chromosome 6 at 29,456,384 bp
  • G to A, chromosome 6 at 41,698,420 bp
  • A to G, chromosome 6 at 42,597,185 bp
  • G to A, chromosome 6 at 111,358,426 bp
  • T to A, chromosome 6 at 120,218,967 bp
  • A to G, chromosome 6 at 128,885,007 bp
  • T to A, chromosome 7 at 29,109,812 bp
  • T to A, chromosome 8 at 37,639,984 bp
  • C to A, chromosome 8 at 48,367,377 bp
  • C to T, chromosome 8 at 84,082,720 bp
  • C to A, chromosome 8 at 120,167,303 bp
  • C to T, chromosome 9 at 75,893,709 bp
  • T to C, chromosome 9 at 109,100,772 bp
  • G to T, chromosome 9 at 116,131,601 bp
  • T to C, chromosome 10 at 59,750,521 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • C to T, chromosome 11 at 70,660,800 bp
  • A to G, chromosome 11 at 72,437,738 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • A to G, chromosome 12 at 98,780,065 bp
  • T to A, chromosome 12 at 112,123,412 bp
  • G to A, chromosome 13 at 55,156,651 bp
  • A to G, chromosome 13 at 70,592,650 bp
  • T to C, chromosome 14 at 51,255,563 bp
  • C to A, chromosome 15 at 39,123,224 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • C to T, chromosome 15 at 79,762,447 bp
  • T to C, chromosome 15 at 94,529,486 bp
  • C to T, chromosome 16 at 10,523,792 bp
  • T to C, chromosome 16 at 88,707,538 bp
  • A to C, chromosome 18 at 42,578,867 bp
  • A to T, chromosome 18 at 56,957,856 bp
  • T to A, chromosome 19 at 4,143,747 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5431 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042847-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.