Strain Name:
C57BL/6J-MtgxR4885Btlr/Mmmh
Stock Number:
042851-MU
Citation ID:
RRID:MMRRC_042851-MU
Other Names:
R4885 (G1), C57BL/6J-MtgxR4885Btlr
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Chat
Name: choline acetyltransferase
Synonyms: B230380D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12647
VEGA: 14
HGNC: HGNC:1912
Homologene: 40693
Nnat
Name: neuronatin
Synonyms: Peg5, 5730414I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18111
HGNC: HGNC:7860
Homologene: 36176
Itk
Name: IL2 inducible T cell kinase
Synonyms: Tcsk, Tsk, Emt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100710
Homologene: 41001
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: C130061D10Rik, Phr1, Pam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22129
Homologene: 2487
Tcf12
Name: transcription factor 12
Synonyms: bHLHb20, HEBAlt, ALF1, HTF4, ME1, HTF-4, REB, HEB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21406
Homologene: 40774
Vrk1
Name: vaccinia related kinase 1
Synonyms: 51PK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22367
VEGA: 12
Homologene: 2541
Rack1
Name: receptor for activated C kinase 1
Synonyms: Gnb2-rs1, GB-like, Gnb2l1, p205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14694
HGNC: HGNC:4399
Homologene: 4446
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: opm, A630086P08Rik, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: MICAL-3, C130040D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194401
Homologene: 85288
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: Falz, 9430093H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Impact
Name: impact, RWD domain protein
Synonyms: E430016J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16210
VEGA: 18
Homologene: 5490
Smg6
Name: SMG6 nonsense mediated mRNA decay factor
Synonyms: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103677
Homologene: 23024
Rnf216
Name: ring finger protein 216
Synonyms: 2810055G22Rik, Ubce7ip1, F830018F18Rik, UIP83
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108086
Homologene: 19442
C77080
Name: expressed sequence C77080
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 97130
Homologene: 45837
Ift80
Name: intraflagellar transport 80
Synonyms: Wdr56, 4921524P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68259
Homologene: 12253
Mettl13
Name: methyltransferase 13, eEF1A lysine and N-terminal methyltransferase
Synonyms: 5630401D24Rik, Eef1aknmt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71449
Homologene: 32285
Rbm15
Name: RNA binding motif protein 15
Synonyms: C230088J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229700
Homologene: 23383
Zc3h18
Name: zinc finger CCCH-type containing 18
Synonyms: 5830416A07Rik, 1190001B23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76014
Homologene: 44894
Macrod2
Name: mono-ADP ribosylhydrolase 2
Synonyms: 1110033L15Rik, 2900006F19Rik, 2610107G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72899
Homologene: 85987
Sc5d
Name: sterol-C5-desaturase
Synonyms: A830073K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235293
VEGA: 9
Homologene: 5044
Atp5o
Name: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Synonyms: D12Wsu28e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 28080
HGNC: HGNC:850
Homologene: 1283
Abcf3
Name: ATP-binding cassette, sub-family F (GCN20), member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27406
HGNC: HGNC:72
Homologene: 22784
Anapc10
Name: anaphase promoting complex subunit 10
Synonyms: A830003M23Rik, 1500026N15Rik, Apc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 68999
Homologene: 32238
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72205
Homologene: 8125
Scpep1
Name: serine carboxypeptidase 1
Synonyms: 2410018F01Rik, 4833411K15Rik, Risc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74617
Homologene: 41443
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: D12Ertd364e, C730036B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381591
Homologene: 135709
Stk32b
Name: serine/threonine kinase 32B
Synonyms: YANK2, Stk32, STKG6, 2510009F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 64293
Homologene: 48654
Wscd1
Name: WSC domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216881
Homologene: 18590
Pik3ap1
Name: phosphoinositide-3-kinase adaptor protein 1
Synonyms: 1810044J04Rik, BCAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83490
VEGA: 19
Homologene: 12848
Fgd3
Name: FYVE, RhoGEF and PH domain containing 3
Synonyms: 5830461L01Rik, ZFYVE5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 30938
Homologene: 22925
Pkp1
Name: plakophilin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18772
HGNC: HGNC:9023
Homologene: 253
Myo16
Name: myosin XVI
Synonyms: C230040D10Rik, BM140241, Nyap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 637515
Homologene: 19080
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Irak3
Name: interleukin-1 receptor-associated kinase 3
Synonyms: IRAK-M, 4833428C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73914
Homologene: 36215
Kcnq4
Name: potassium voltage-gated channel, subfamily Q, member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 60613
HGNC: HGNC:6298
Homologene: 78107
Sh3pxd2a
Name: SH3 and PX domains 2A
Synonyms: Fish, 2310014D11Rik, Sh3md1, Tks5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14218
VEGA: 19
Homologene: 7317
Or5h18
Name: olfactory receptor family 5 subfamily H member 18
Synonyms: GA_x54KRFPKG5P-55257214-55256285, Olfr186, MOR183-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258318
Homologene: 133671
Atosa
Name: atos homolog A
Synonyms: 6330415I01Rik, C130047D21Rik, BC031353, Fam214a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235493
VEGA: 9
Homologene: 35065
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Ttc41
Name: tetratricopeptide repeat domain 41
Synonyms: Gnn, BC030307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103220
VEGA: 10
Homologene: 52968
Phf19
Name: PHD finger protein 19
Synonyms: 3321402G02Rik, 3110009G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74016
Homologene: 12524
Slc38a7
Name: solute carrier family 38, member 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234595
Homologene: 41237
Slc1a4
Name: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Synonyms: ASCT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 55963
Homologene: 20655
Adck1
Name: aarF domain containing kinase 1
Synonyms: 2610005A10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 72113
VEGA: 12
Homologene: 6493
Ccl20
Name: chemokine (C-C motif) ligand 20
Synonyms: Scya20, MIP-3[a], exodus-1, CKb4, MIP-3A, MIP3A, ST38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20297
Homologene: 3375
Nkx6-3
Name: NK6 homeobox 3
Synonyms: Nkx6.3, 9130417I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74561
Homologene: 46375
Tas2r137
Name: taste receptor, type 2, member 137
Synonyms: mt2r41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 574417
Homologene: 23231
Foxs1
Name: forkhead box S1
Synonyms: Fkh3, FREAC10, Fkhl18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14239
HGNC: HGNC:3735
Homologene: 20871
Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: Bai1, B830018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2810470M17Rik, 2610019B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67242
Homologene: 11711
Ivl
Name: involucrin
Synonyms: 1110019C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16447
HGNC: HGNC:6187
Homologene: 137207
Fam234a
Name: family with sequence similarity 234, member A
Synonyms: Itfg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106581
Homologene: 12932
Or5an1c
Name: olfactory receptor family 5 subfamily AN member 1C
Synonyms: Olfr262, GA_x6K02T2N4A9-18144-19082, MOR214-9, MOR214-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258683
Cr2
Name: complement receptor 2
Synonyms: C3DR, CD21, Cr1, Cr-2, Cr-1, CD35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Eef2k
Name: eukaryotic elongation factor-2 kinase
Synonyms: eEF-2K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13631
Homologene: 7299
Insl6
Name: insulin-like 6
Synonyms: RIF1, relaxin/insulin-like factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 27356
HGNC: HGNC:6089
Homologene: 5197
Mfsd13b
Name: major facilitator superfamily domain containing 13B
Synonyms: 4933427G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74466
Homologene: 62261
Gfi1
Name: growth factor independent 1 transcription repressor
Synonyms: Gfi-1, Pal-1, Pal1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14581
HGNC: HGNC:4237
Homologene: 3854
Or2y16
Name: olfactory receptor family 2 subfamily Y member 16
Synonyms: Olfr1388, MOR256-28, GA_x6K02T2QP88-5991012-5990077
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258459
Homologene: 74258
Ccnl1
Name: cyclin L1
Synonyms: 2610030E23Rik, ania-6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56706
Homologene: 10541
Fabp9
Name: fatty acid binding protein 9, testis
Synonyms: 1700007P10Rik, Tlbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 21884
HGNC: HGNC:3563
Homologene: 40776
H3c13
Name: H3 clustered histone 13
Synonyms: H3-616, Hist2h3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 319154
Homologene: 136775
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,070,488 bp
  • T to A, chromosome 1 at 83,117,859 bp
  • A to G, chromosome 1 at 135,918,952 bp
  • T to C, chromosome 1 at 162,537,268 bp
  • T to A, chromosome 1 at 195,158,731 bp
  • A to T, chromosome 2 at 34,899,706 bp
  • T to C, chromosome 2 at 41,468,893 bp
  • T to C, chromosome 2 at 52,286,046 bp
  • A to T, chromosome 2 at 82,988,094 bp
  • A to G, chromosome 2 at 140,420,065 bp
  • T to C, chromosome 2 at 152,932,381 bp
  • G to A, chromosome 2 at 157,561,758 bp
  • T to C, chromosome 3 at 10,194,678 bp
  • C to T, chromosome 3 at 65,956,899 bp
  • A to G, chromosome 3 at 68,950,496 bp
  • A to T, chromosome 3 at 92,572,411 bp
  • G to T, chromosome 3 at 96,268,961 bp
  • A to G, chromosome 3 at 107,332,254 bp
  • T to A, chromosome 4 at 88,627,982 bp
  • C to T, chromosome 4 at 98,737,311 bp
  • G to A, chromosome 4 at 120,713,063 bp
  • C to T, chromosome 4 at 129,224,445 bp
  • T to C, chromosome 5 at 37,466,797 bp
  • C to A, chromosome 5 at 107,723,286 bp
  • A to T, chromosome 5 at 143,090,580 bp
  • C to T, chromosome 5 at 150,716,462 bp
  • A to G, chromosome 6 at 40,491,400 bp
  • G to A, chromosome 6 at 120,935,253 bp
  • G to A, chromosome 7 at 19,204,010 bp
  • G to A, chromosome 7 at 120,891,932 bp
  • A to T, chromosome 7 at 120,991,488 bp
  • T to A, chromosome 8 at 10,438,892 bp
  • C to A, chromosome 8 at 23,153,898 bp
  • C to T, chromosome 8 at 79,729,150 bp
  • T to C, chromosome 8 at 95,848,602 bp
  • C to T, chromosome 8 at 122,401,706 bp
  • T to C, chromosome 9 at 42,255,626 bp
  • C to T, chromosome 9 at 71,858,840 bp
  • T to C, chromosome 9 at 75,006,367 bp
  • A to T, chromosome 9 at 108,107,527 bp
  • T to G, chromosome 10 at 86,759,102 bp
  • T to A, chromosome 10 at 120,182,681 bp
  • A to C, chromosome 11 at 20,304,384 bp
  • C to T, chromosome 11 at 46,336,344 bp
  • C to A, chromosome 11 at 48,805,636 bp
  • C to T, chromosome 11 at 49,444,622 bp
  • T to C, chromosome 11 at 71,217,884 bp
  • C to T, chromosome 11 at 71,760,146 bp
  • T to C, chromosome 11 at 75,041,918 bp
  • A to G, chromosome 11 at 88,935,911 bp
  • C to T, chromosome 11 at 107,074,648 bp
  • A to G, chromosome 12 at 51,800,722 bp
  • C to T, chromosome 12 at 65,102,643 bp
  • G to A, chromosome 12 at 88,441,095 bp
  • G to A, chromosome 12 at 106,057,972 bp
  • G to A, chromosome 13 at 49,263,989 bp
  • C to G, chromosome 14 at 32,454,610 bp
  • T to A, chromosome 14 at 77,720,850 bp
  • T to C, chromosome 14 at 103,145,946 bp
  • A to T, chromosome 15 at 74,572,162 bp
  • A to G, chromosome 16 at 20,551,675 bp
  • A to G, chromosome 16 at 59,027,155 bp
  • A to T, chromosome 16 at 91,926,459 bp
  • T to C, chromosome 16 at 94,419,465 bp
  • G to C, chromosome 16 at 94,426,831 bp
  • T to C, chromosome 17 at 26,213,585 bp
  • G to A, chromosome 17 at 32,141,377 bp
  • G to A, chromosome 17 at 80,227,898 bp
  • C to G, chromosome 18 at 12,986,373 bp
  • G to C, chromosome 18 at 49,878,795 bp
  • T to G, chromosome 19 at 12,240,718 bp
  • C to T, chromosome 19 at 29,325,156 bp
  • T to C, chromosome 19 at 41,375,926 bp
  • A to G, chromosome 19 at 47,268,693 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4885 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042851-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.