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Strain Name:
C57BL/6J-MtgxR5259Btlr/Mmmh
Stock Number:
042856-MU
Citation ID:
RRID:MMRRC_042856-MU
Other Names:
R5259 (G1), C57BL/6J-MtgxR5259Btlr
Major Collection:

Strain Information

Bdnf
Name: brain derived neurotrophic factor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12064
HGNC: HGNC:1033
Homologene: 7245
Slc35a1
Name: solute carrier family 35 (CMP-sialic acid transporter), member 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 24060
Homologene: 38181
Mta3
Name: metastasis associated 3
Synonyms: 1110002J22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 116871
Homologene: 14282
Kcnc4
Name: potassium voltage gated channel, Shaw-related subfamily, member 4
Synonyms: Kv3.4, Kcr2-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99738
HGNC: HGNC:6236
Homologene: 68427
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Rheb
Name: Ras homolog enriched in brain
Synonyms: Rheb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19744
Homologene: 123916
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 32,545,517 bp
  • A to G, chromosome 1 at 72,277,285 bp
  • G to T, chromosome 1 at 82,453,893 bp
  • A to G, chromosome 1 at 97,721,208 bp
  • A to G, chromosome 1 at 105,721,376 bp
  • A to G, chromosome 1 at 121,567,789 bp
  • T to A, chromosome 1 at 138,112,255 bp
  • C to T, chromosome 1 at 139,740,495 bp
  • A to G, chromosome 2 at 109,723,982 bp
  • T to A, chromosome 2 at 114,197,193 bp
  • T to C, chromosome 2 at 132,906,765 bp
  • A to C, chromosome 3 at 40,916,890 bp
  • C to T, chromosome 3 at 51,484,612 bp
  • A to G, chromosome 3 at 107,448,085 bp
  • C to T, chromosome 4 at 34,683,322 bp
  • T to A, chromosome 4 at 49,490,070 bp
  • C to T, chromosome 5 at 22,103,397 bp
  • A to C, chromosome 5 at 24,803,745 bp
  • T to A, chromosome 5 at 28,352,774 bp
  • C to T, chromosome 5 at 32,938,291 bp
  • A to T, chromosome 5 at 105,050,979 bp
  • T to C, chromosome 6 at 32,517,021 bp
  • T to A, chromosome 6 at 57,432,065 bp
  • A to T, chromosome 6 at 85,830,891 bp
  • A to G, chromosome 7 at 16,945,274 bp
  • T to C, chromosome 7 at 43,637,112 bp
  • T to A, chromosome 7 at 79,694,723 bp
  • T to A, chromosome 7 at 103,250,574 bp
  • G to A, chromosome 7 at 128,148,278 bp
  • T to A, chromosome 8 at 24,810,834 bp
  • T to C, chromosome 8 at 45,962,336 bp
  • A to G, chromosome 8 at 74,892,845 bp
  • A to T, chromosome 8 at 126,389,133 bp
  • A to G, chromosome 9 at 19,712,813 bp
  • A to T, chromosome 9 at 78,710,152 bp
  • A to G, chromosome 9 at 109,520,312 bp
  • G to C, chromosome 9 at 122,166,320 bp
  • C to G, chromosome 10 at 70,441,063 bp
  • A to T, chromosome 10 at 74,396,372 bp
  • T to C, chromosome 10 at 114,816,817 bp
  • A to G, chromosome 11 at 58,701,952 bp
  • A to C, chromosome 11 at 58,701,953 bp
  • T to C, chromosome 12 at 28,675,545 bp
  • T to A, chromosome 12 at 115,834,177 bp
  • C to T, chromosome 13 at 9,158,184 bp
  • C to T, chromosome 13 at 27,436,196 bp
  • G to T, chromosome 13 at 67,669,686 bp
  • T to G, chromosome 13 at 94,232,086 bp
  • G to A, chromosome 14 at 14,116,602 bp
  • T to C, chromosome 14 at 33,944,831 bp
  • A to G, chromosome 14 at 123,515,651 bp
  • T to C, chromosome 15 at 10,515,150 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • A to T, chromosome 15 at 76,301,210 bp
  • T to A, chromosome 15 at 98,880,583 bp
  • G to A, chromosome 16 at 29,456,610 bp
  • G to A, chromosome 16 at 48,630,769 bp
  • A to T, chromosome 17 at 25,796,540 bp
  • A to C, chromosome 17 at 56,660,235 bp
  • A to G, chromosome 17 at 75,363,362 bp
  • T to A, chromosome 17 at 79,668,017 bp
  • A to G, chromosome 17 at 83,804,574 bp
  • A to C, chromosome 18 at 12,465,508 bp
  • T to C, chromosome 18 at 34,314,290 bp
  • T to A, chromosome 18 at 59,410,246 bp
  • G to A, chromosome 19 at 26,886,406 bp
  • A to G, chromosome 19 at 38,199,662 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5259 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042856-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.