Strain Name:
C57BL/6J-MtgxR5281Btlr/Mmmh
Stock Number:
042866-MU
Citation ID:
RRID:MMRRC_042866-MU
Other Names:
R5281 (G1), C57BL/6J-MtgxR5281Btlr
Major Collection:

Gene Information

Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22359
Homologene: 443
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Dvl2
Name: dishevelled segment polarity protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13543
HGNC: HGNC:3086
Homologene: 20927
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100683
Homologene: 39246
Slitrk6
Name: SLIT and NTRK-like family, member 6
Synonyms: 4832410J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239250
VEGA: 14
Homologene: 12986
Aff4
Name: AF4/FMR2 family, member 4
Synonyms: Alf4, Laf4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 93736
Homologene: 8683
Eif2d
Name: eukaryotic translation initiation factor 2D
Synonyms: D1Ertd5e, Lgtn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16865
HGNC: HGNC:6583
Homologene: 38244
Gls
Name: glutaminase
Synonyms: B230365M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14660
HGNC: HGNC:4331
Homologene: 22726
Nif3l1
Name: Ngg1 interacting factor 3-like 1 (S. pombe)
Synonyms: 1110030G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 65102
Homologene: 5881
Ano10
Name: anoctamin 10
Synonyms: Tmem16k
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102566
VEGA: 9
Homologene: 14314
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Cenpe
Name: centromere protein E
Synonyms: N-7 kinesin, CENP-E, 312kDa, Kif10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229841
HGNC: HGNC:1856
Homologene: 20429
Ints7
Name: integrator complex subunit 7
Synonyms: 5930412E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77065
Homologene: 9136
Col4a4
Name: collagen, type IV, alpha 4
Synonyms: [a]4(IV), E130010M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12829
HGNC: HGNC:2206
Homologene: 20071
Slfn4
Name: schlafen 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20558
Homologene: 133236
Adamtsl3
Name: ADAMTS-like 3
Synonyms: punctin-2, 9230119C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269959
Homologene: 18912
Xylt2
Name: xylosyltransferase II
Synonyms: E030002B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217119
Homologene: 23349
Ggct
Name: gamma-glutamyl cyclotransferase
Synonyms: Ggc, Gctg, A030007L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110175
Homologene: 11437
Atp10b
Name: ATPase, class V, type 10B
Synonyms: 5930426O13Rik, 9030605H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319767
Homologene: 70969
Btn2a2
Name: butyrophilin, subfamily 2, member A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238555
HGNC: HGNC:1137
Homologene: 56012
Whrn
Name: whirlin
Synonyms: 1110035G07Rik, wi, C430046P22Rik, Dfnb31, Ush2d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73750
Homologene: 18739
Shpk
Name: sedoheptulokinase
Synonyms: 4930431K22Rik, Carkl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74637
HGNC: HGNC:1492
Homologene: 8319
Fsd2
Name: fibronectin type III and SPRY domain containing 2
Synonyms: Spryd1, 9830160G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244091
Homologene: 18252
Nr0b2
Name: nuclear receptor subfamily 0, group B, member 2
Synonyms: small heterodimer partner, SHP-1, SHP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 23957
HGNC: HGNC:7961
Homologene: 8030
Krt17
Name: keratin 17
Synonyms: K17, Krt1-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16667
HGNC: HGNC:6427
Homologene: 363
Zfp800
Name: zinc finger protein 800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 627049
Homologene: 18529
Fap
Name: fibroblast activation protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14089
HGNC: HGNC:3590
Homologene: 48282
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 639774
Homologene: 106613
Gusb
Name: glucuronidase, beta
Synonyms: g, Gur, Gut, asd, adipose storage deficiency, Gus-u, Gus-t, Gus-s, Gus-r, Gus
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 110006
HGNC: HGNC:4696
Homologene: 37271
C2cd4c
Name: C2 calcium-dependent domain containing 4C
Synonyms: LOC237397, 4932409I22Rik, Fam148c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237397
VEGA: 10
Homologene: 19012
Pcdhb21
Name: protocadherin beta 21
Synonyms: Pcdhb18, PcdhbU
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93892
HGNC: HGNC:8682
Homologene: 134302
Cant1
Name: calcium activated nucleotidase 1
Synonyms: 5830420C20Rik, Apy1h, Shapy, D11Bwg0554e, SCAN-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76025
Homologene: 69460
Gm11595
Name: predicted gene 11595
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100040276
Homologene: 124486
Supt4a
Name: SPT4A, DSIF elongation factor subunit
Synonyms: Supt4h, Supt4h1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20922
Homologene: 68303
Olfr609
Name: olfactory receptor 609
Synonyms: GA_x6K02T2PBJ9-6207735-6206776, MOR9-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259086
Homologene: 64952
She
Name: src homology 2 domain-containing transforming protein E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214547
Homologene: 18186
Epha10
Name: Eph receptor A10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230735
Homologene: 67804
Dnajc5b
Name: DnaJ heat shock protein family (Hsp40) member C5 beta
Synonyms: 1700008A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66326
Homologene: 32634
Prr33
Name: proline rich 33
Synonyms: ENSMUSG00000043795, Gm14492
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 677289
Mfsd4b4
Name: major facilitator superfamily domain containing 4B4
Synonyms: AA474331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 213332
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 34,257,782 bp
  • A to T, chromosome 1 at 52,191,157 bp
  • A to G, chromosome 1 at 58,455,771 bp
  • C to T, chromosome 1 at 77,374,867 bp
  • C to T, chromosome 1 at 82,493,591 bp
  • A to G, chromosome 1 at 131,173,343 bp
  • T to C, chromosome 1 at 191,615,771 bp
  • T to A, chromosome 2 at 20,849,316 bp
  • C to T, chromosome 2 at 62,532,961 bp
  • T to C, chromosome 3 at 19,610,560 bp
  • A to G, chromosome 3 at 89,849,581 bp
  • A to C, chromosome 3 at 135,230,150 bp
  • T to C, chromosome 4 at 63,418,427 bp
  • C to A, chromosome 4 at 111,950,193 bp
  • A to T, chromosome 4 at 124,913,988 bp
  • A to G, chromosome 4 at 133,556,024 bp
  • T to C, chromosome 5 at 129,998,526 bp
  • T to C, chromosome 5 at 144,813,503 bp
  • A to T, chromosome 6 at 28,243,166 bp
  • G to A, chromosome 6 at 54,985,975 bp
  • T to C, chromosome 7 at 81,552,985 bp
  • A to G, chromosome 7 at 82,528,934 bp
  • T to C, chromosome 7 at 103,491,973 bp
  • T to C, chromosome 7 at 131,082,619 bp
  • T to C, chromosome 7 at 142,492,119 bp
  • A to G, chromosome 9 at 122,261,486 bp
  • T to C, chromosome 10 at 39,892,471 bp
  • G to A, chromosome 10 at 79,613,044 bp
  • T to C, chromosome 11 at 43,254,336 bp
  • A to G, chromosome 11 at 53,372,288 bp
  • T to C, chromosome 11 at 70,005,223 bp
  • T to C, chromosome 11 at 73,215,120 bp
  • T to G, chromosome 11 at 83,187,199 bp
  • A to G, chromosome 11 at 87,740,576 bp
  • A to G, chromosome 11 at 94,668,790 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • G to A, chromosome 11 at 100,260,701 bp
  • A to T, chromosome 11 at 118,408,870 bp
  • A to G, chromosome 13 at 23,478,832 bp
  • C to T, chromosome 14 at 110,750,373 bp
  • T to C, chromosome 18 at 37,513,935 bp
  • A to C, chromosome 19 at 27,244,231 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5281 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042866-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.