Strain Name:
C57BL/6J-MtgxR5301Btlr/Mmmh
Stock Number:
042884-MU
Citation ID:
RRID:MMRRC_042884-MU
Other Names:
R5301 (G1), C57BL/6J-MtgxR5301Btlr
Major Collection:

Strain Information

Prkar2b
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: PKARIIbeta, Pkarb2, RII(beta)
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19088
HGNC: HGNC:9392
Homologene: 37666
Bdnf
Name: brain derived neurotrophic factor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12064
HGNC: HGNC:1033
Homologene: 7245
Sgca
Name: sarcoglycan, alpha (dystrophin-associated glycoprotein)
Synonyms: 50DAG, adhalin, Asg
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20391
Homologene: 9
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Hcrtr1
Name: hypocretin (orexin) receptor 1
Synonyms: OX1R
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230777
HGNC: HGNC:4848
Homologene: 37492
Atg14
Name: autophagy related 14
Synonyms: D14Ertd436e, Barkor, D14Ertd114e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504663
VEGA: 14
Homologene: 45423
Pkn2
Name: protein kinase N2
Synonyms: Stk7, Prkcl2, 6030436C20Rik, PRK2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Epcam
Name: epithelial cell adhesion molecule
Synonyms: Egp314, TROP1, EpCAM1, GA733-2, Tacstd1, Ly74, panepithelial glycoprotein 314, gp40, EpCAM, Ep-CAM, EGP-2, CD326
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17075
VEGA: 17
Homologene: 1764
Pacrgl
Name: PARK2 co-regulated-like
Synonyms: 4933428G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66768
VEGA: 5
Homologene: 12030
Itgb3
Name: integrin beta 3
Synonyms: platelet glycoprotein IIIa (GP3A), CD61
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16416
HGNC: HGNC:6156
Homologene: 55444
Ino80d
Name: INO80 complex subunit D
Synonyms: A430093A21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227195
Homologene: 9819
Bcl10
Name: B cell leukemia/lymphoma 10
Synonyms: BCL-10, mE10, cE10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12042
HGNC: HGNC:989
Homologene: 2912
Mplkipl1
Name: M-phase specific PLK1 intereacting protein like 1
Synonyms: Gm7102
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 633057
Mthfd2
Name: methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase
Synonyms: NMDMC
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17768
HGNC: HGNC:7434
Homologene: 21321
Gja1
Name: gap junction protein, alpha 1
Synonyms: Cx43, alpha 1 connexin, connexin43, Gja-1, connexin 43, Cnx43, Cx43alpha1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14609
VEGA: 10
HGNC: HGNC:4274
Homologene: 136
Wnt16
Name: wingless-type MMTV integration site family, member 16
Synonyms: E130309I19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93735
Homologene: 62175
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, Dnhd3, Dnahc2, 2900022L05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Sec14l2
Name: SEC14-like lipid binding 2
Synonyms: tap, 1300013M05Rik, Spf
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67815
Homologene: 8245
Abca4
Name: ATP-binding cassette, sub-family A member 4
Synonyms: Abc10, D430003I15Rik, RmP, Rim protein
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Reg3b
Name: regenerating islet-derived 3 beta
Synonyms: Pap, PAP1, REG-III, RegIII (beta)
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18489
Homologene: 130506
Dock10
Name: dedicator of cytokinesis 10
Synonyms: 9330153B10Rik, A630054M16Rik, ZIZ3, Jr4, Jr5, Zizimin3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Abcb11
Name: ATP-binding cassette, sub-family B member 11
Synonyms: sister of P-glycoprotein, PGY4, ABC16, PFIC2, Lith1, Bsep
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: OTTMUSG00000020804, ENSMUSG00000044873, Heatr7b1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Ifi207
Name: interferon activated gene 207
Synonyms: Pyhin-A, AI607873
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Nrp1
Name: neuropilin 1
Synonyms: Npn1, NPN-1, Neuropilin-1, NP-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18186
HGNC: HGNC:8004
Homologene: 2876
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: E130113P14Rik, bl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231470
Homologene: 23516
Abcc9
Name: ATP-binding cassette, sub-family C member 9
Synonyms: Sur2, SUR2A, SUR2B
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
Ttc41
Name: tetratricopeptide repeat domain 41
Synonyms: Gnn, BC030307
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103220
VEGA: 10
Homologene: 52968
Ppp1r18
Name: protein phosphatase 1, regulatory subunit 18
Synonyms: 2310014H01Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76448
Homologene: 19512
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269152
Homologene: 18623
Akr1c20
Name: aldo-keto reductase family 1, member C20
Synonyms: 2610528B18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 116852
Klc3
Name: kinesin light chain 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232943
Homologene: 14899
Ercc4
Name: excision repair cross-complementing rodent repair deficiency, complementation group 4
Synonyms: Xpf
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50505
HGNC: HGNC:3436
Homologene: 3836
Or9i14
Name: olfactory receptor family 9 subfamily I member 14
Synonyms: GA_x6K02T2RE5P-4147744-4146800, Olfr1499, MOR211-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258792
Homologene: 17393
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81840
Homologene: 56899
Krtap4-23
Name: keratin associated protein 4-23
Synonyms: Gm11596
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 670464
Homologene: 124486
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 215384
Homologene: 68369
Meioc
Name: meiosis specific with coiled-coil domain
Synonyms: LOC380729, Gm1564, LOC268491
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268491
Homologene: 19559
Vmn2r96
Name: vomeronasal 2, receptor 96
Synonyms: EG433070
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 433070
Cyp11a1
Name: cytochrome P450, family 11, subfamily a, polypeptide 1
Synonyms: cholesterol side chain cleavage, Scc, D9Ertd411e, P450scc, cscc, Cyp11a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13070
HGNC: HGNC:2590
Homologene: 37347
Klhl18
Name: kelch-like 18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270201
Homologene: 14805
Cyp3a44
Name: cytochrome P450, family 3, subfamily a, polypeptide 44
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 337924
HGNC: HGNC:2638
Homologene: 133568
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74691
Homologene: 14311
Gata5
Name: GATA binding protein 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14464
Homologene: 32031
Or11g1
Name: olfactory receptor family 11 subfamily G member 1
Synonyms: Olfr738, GA_x6K02T2PMLR-6110726-6111661, MOR106-3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258662
Homologene: 74220
Vmn1r234
Name: vomeronasal 1 receptor 234
Synonyms: V1rf1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171232
Desi2
Name: desumoylating isopeptidase 2
Synonyms: Pppde1, 5830417C01Rik, Fam152a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78825
Homologene: 56741
Or1b1
Name: olfactory receptor family 1 subfamily B member 1
Synonyms: MOR158-1, Olfr362, GA_x6K02T2NLDC-33797415-33796462
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259053
HGNC: HGNC:8181
Homologene: 17479
Zfp607b
Name: zinc finger protein 607B
Synonyms: C030039L03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 112415
Homologene: 134321
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, C76977, Tmem181, Gpr178
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 77106
Homologene: 44787
Asb1
Name: ankyrin repeat and SOCS box-containing 1
Synonyms: 1700054C17Rik, 1700029O08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 65247
Homologene: 9390
Ccl6
Name: C-C motif chemokine ligand 6
Synonyms: c10, MRP-1, Scya6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20305
Homologene: 22507
Gm6189
Name: predicted gene 6189
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 620899
Gm26765
Name: predicted gene, 26765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,074,419 bp
  • A to T, chromosome 1 at 80,384,437 bp
  • T to C, chromosome 1 at 80,648,256 bp
  • A to G, chromosome 1 at 88,258,664 bp
  • A to G, chromosome 1 at 91,554,753 bp
  • G to A, chromosome 1 at 173,729,411 bp
  • A to G, chromosome 1 at 178,244,386 bp
  • A to G, chromosome 1 at 178,530,668 bp
  • C to T, chromosome 2 at 37,105,198 bp
  • T to C, chromosome 2 at 69,286,847 bp
  • T to C, chromosome 2 at 109,723,539 bp
  • T to C, chromosome 2 at 180,333,993 bp
  • T to C, chromosome 3 at 122,102,853 bp
  • A to C, chromosome 3 at 142,839,206 bp
  • A to G, chromosome 3 at 145,930,587 bp
  • G to T, chromosome 4 at 130,137,670 bp
  • A to G, chromosome 5 at 36,039,390 bp
  • T to C, chromosome 5 at 48,374,472 bp
  • C to T, chromosome 5 at 96,657,266 bp
  • A to G, chromosome 5 at 145,788,516 bp
  • T to A, chromosome 6 at 22,297,849 bp
  • T to A, chromosome 6 at 78,371,260 bp
  • C to A, chromosome 6 at 83,310,483 bp
  • G to A, chromosome 6 at 142,590,481 bp
  • T to C, chromosome 7 at 19,396,349 bp
  • A to G, chromosome 7 at 27,703,747 bp
  • A to T, chromosome 7 at 28,093,674 bp
  • A to G, chromosome 7 at 51,658,771 bp
  • T to G, chromosome 8 at 128,434,197 bp
  • A to G, chromosome 9 at 58,019,261 bp
  • G to C, chromosome 9 at 110,436,127 bp
  • T to C, chromosome 10 at 56,388,379 bp
  • T to C, chromosome 10 at 86,719,520 bp
  • A to T, chromosome 10 at 91,149,788 bp
  • G to A, chromosome 11 at 4,118,727 bp
  • A to G, chromosome 11 at 59,135,408 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • C to T, chromosome 11 at 83,588,864 bp
  • C to T, chromosome 11 at 94,963,331 bp
  • C to A, chromosome 11 at 99,793,021 bp
  • A to T, chromosome 11 at 102,680,045 bp
  • A to G, chromosome 11 at 104,633,654 bp
  • A to C, chromosome 12 at 31,975,928 bp
  • T to A, chromosome 12 at 112,036,529 bp
  • T to A, chromosome 13 at 4,523,280 bp
  • G to A, chromosome 14 at 47,568,199 bp
  • T to C, chromosome 14 at 50,413,573 bp
  • T to C, chromosome 16 at 13,130,686 bp
  • T to C, chromosome 17 at 6,295,795 bp
  • T to A, chromosome 17 at 18,597,688 bp
  • G to T, chromosome 17 at 21,229,327 bp
  • A to G, chromosome 17 at 35,868,345 bp
  • T to A, chromosome 17 at 87,636,877 bp
  • G to A, chromosome 19 at 13,815,569 bp
  • A to G, chromosome 19 at 56,379,109 bp
  • C to T, chromosome 19 at 61,175,926 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5301 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042884-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.