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Strain Name:
C57BL/6J-MtgxR5306Btlr/Mmmh
Stock Number:
042889-MU
Citation ID:
RRID:MMRRC_042889-MU
Other Names:
R5306 (G1), C57BL/6J-MtgxR5306Btlr
Major Collection:

Strain Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
Gfap
Name: glial fibrillary acidic protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14580
HGNC: HGNC:4235
Homologene: 1554
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Rc3h2
Name: ring finger and CCCH-type zinc finger domains 2
Synonyms: Rnf164, D930043C02Rik, 2900024N03Rik, 9430019J22Rik, Mnab
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 319817
Homologene: 28276
Wdr70
Name: WD repeat domain 70
Synonyms: 4833422F06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 545085
VEGA: 15
Homologene: 9972
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 54,926,203 bp
  • T to C, chromosome 1 at 89,144,275 bp
  • G to C, chromosome 1 at 90,801,531 bp
  • T to A, chromosome 1 at 170,349,399 bp
  • A to T, chromosome 2 at 14,219,164 bp
  • A to G, chromosome 2 at 37,381,870 bp
  • T to A, chromosome 2 at 91,646,345 bp
  • A to T, chromosome 2 at 94,423,466 bp
  • T to A, chromosome 2 at 130,419,171 bp
  • T to C, chromosome 2 at 175,169,579 bp
  • A to G, chromosome 2 at 181,574,367 bp
  • G to A, chromosome 3 at 84,451,418 bp
  • C to T, chromosome 3 at 89,223,071 bp
  • G to T, chromosome 3 at 97,641,760 bp
  • G to T, chromosome 4 at 21,931,557 bp
  • C to T, chromosome 4 at 133,211,179 bp
  • T to C, chromosome 4 at 148,562,392 bp
  • C to A, chromosome 4 at 154,006,242 bp
  • T to C, chromosome 4 at 155,875,208 bp
  • T to C, chromosome 5 at 23,499,333 bp
  • T to A, chromosome 5 at 107,345,144 bp
  • T to C, chromosome 5 at 112,355,044 bp
  • A to G, chromosome 5 at 129,769,367 bp
  • T to C, chromosome 6 at 32,206,121 bp
  • G to A, chromosome 6 at 90,662,878 bp
  • A to G, chromosome 7 at 5,058,097 bp
  • A to C, chromosome 7 at 10,170,745 bp
  • A to G, chromosome 7 at 28,091,818 bp
  • A to T, chromosome 7 at 30,265,263 bp
  • A to T, chromosome 7 at 48,312,192 bp
  • C to T, chromosome 7 at 56,184,961 bp
  • A to G, chromosome 7 at 87,438,014 bp
  • A to C, chromosome 7 at 97,509,678 bp
  • CGGCGGC to CGGCGGCGGGGGCGGC, chromosome 7 at 97,579,929 bp
  • T to A, chromosome 7 at 99,688,991 bp
  • A to T, chromosome 7 at 135,714,001 bp
  • T to A, chromosome 8 at 55,871,757 bp
  • T to A, chromosome 8 at 78,251,910 bp
  • T to C, chromosome 10 at 9,799,991 bp
  • A to C, chromosome 10 at 129,873,941 bp
  • A to C, chromosome 11 at 42,133,552 bp
  • T to A, chromosome 11 at 48,915,555 bp
  • A to T, chromosome 11 at 59,372,995 bp
  • T to C, chromosome 11 at 67,186,556 bp
  • A to G, chromosome 11 at 75,402,158 bp
  • T to A, chromosome 11 at 83,035,529 bp
  • T to C, chromosome 11 at 98,428,206 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • A to G, chromosome 11 at 102,895,748 bp
  • G to A, chromosome 11 at 106,778,970 bp
  • G to A, chromosome 12 at 112,601,553 bp
  • T to A, chromosome 13 at 67,413,130 bp
  • G to A, chromosome 13 at 76,147,767 bp
  • G to T, chromosome 13 at 112,851,555 bp
  • G to A, chromosome 14 at 26,911,909 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • A to G, chromosome 14 at 49,929,550 bp
  • G to A, chromosome 14 at 62,670,933 bp
  • T to C, chromosome 15 at 7,924,273 bp
  • C to A, chromosome 15 at 9,457,790 bp
  • T to C, chromosome 15 at 31,582,069 bp
  • A to T, chromosome 16 at 59,559,993 bp
  • T to C, chromosome 17 at 6,601,811 bp
  • C to A, chromosome 17 at 32,883,653 bp
  • G to A, chromosome 17 at 33,760,269 bp
  • G to A, chromosome 18 at 37,011,058 bp
  • A to G, chromosome 19 at 23,259,645 bp
  • A to G, chromosome 19 at 29,729,830 bp
  • A to G, chromosome 19 at 34,587,807 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5306 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042889-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.