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Strain Name:
C57BL/6J-MtgxR5308Btlr/Mmmh
Stock Number:
042891-MU
Citation ID:
RRID:MMRRC_042891-MU
Other Names:
R5308 (G1), C57BL/6J-MtgxR5308Btlr
Major Collection:

Strain Information

Eno2
Name: enolase 2, gamma neuronal
Synonyms: Eno-2, D6Ertd375e, NSE
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13807
HGNC: HGNC:3353
Homologene: 74414
Paics
Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase
Synonyms: ADE2H1, PAIS, AIRC, 2610511I09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67054
HGNC: HGNC:8587
Homologene: 4710
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840
Cdk13
Name: cyclin dependent kinase 13
Synonyms: 2310015O17Rik, Cdc2l5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69562
VEGA: 13
HGNC: HGNC:1733
Homologene: 135707
Xpa
Name: xeroderma pigmentosum, complementation group A
Synonyms: Xpac
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22590
Homologene: 37298
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: RFC140, Alp145, 140kDa, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,389,409 bp
  • G to C, chromosome 1 at 90,801,531 bp
  • T to G, chromosome 1 at 170,965,710 bp
  • T to C, chromosome 2 at 19,524,081 bp
  • T to A, chromosome 2 at 36,640,694 bp
  • T to C, chromosome 2 at 88,683,405 bp
  • C to T, chromosome 2 at 111,564,554 bp
  • C to T, chromosome 2 at 129,200,311 bp
  • G to A, chromosome 2 at 146,109,988 bp
  • C to A, chromosome 2 at 156,144,553 bp
  • T to C, chromosome 3 at 106,001,103 bp
  • C to G, chromosome 3 at 124,406,350 bp
  • T to A, chromosome 4 at 46,185,659 bp
  • A to T, chromosome 4 at 48,182,851 bp
  • C to A, chromosome 4 at 99,034,486 bp
  • A to G, chromosome 4 at 126,035,344 bp
  • T to A, chromosome 5 at 65,279,461 bp
  • T to C, chromosome 5 at 76,956,632 bp
  • T to C, chromosome 6 at 66,537,691 bp
  • T to C, chromosome 6 at 81,943,543 bp
  • G to A, chromosome 6 at 90,662,878 bp
  • A to T, chromosome 6 at 108,356,511 bp
  • C to A, chromosome 6 at 124,767,093 bp
  • T to C, chromosome 6 at 130,334,307 bp
  • G to A, chromosome 6 at 132,700,517 bp
  • T to A, chromosome 7 at 34,245,755 bp
  • T to C, chromosome 7 at 44,500,182 bp
  • T to A, chromosome 7 at 131,041,021 bp
  • G to T, chromosome 8 at 121,917,686 bp
  • A to T, chromosome 9 at 21,140,764 bp
  • C to T, chromosome 9 at 26,764,759 bp
  • T to C, chromosome 9 at 31,429,084 bp
  • T to C, chromosome 9 at 49,037,201 bp
  • T to C, chromosome 9 at 50,822,711 bp
  • T to A, chromosome 9 at 59,863,961 bp
  • T to A, chromosome 9 at 62,755,769 bp
  • T to A, chromosome 9 at 65,558,675 bp
  • T to C, chromosome 9 at 92,198,512 bp
  • G to A, chromosome 9 at 109,789,449 bp
  • G to A, chromosome 10 at 70,002,565 bp
  • A to C, chromosome 10 at 76,356,325 bp
  • T to C, chromosome 10 at 84,528,374 bp
  • T to A, chromosome 10 at 84,947,747 bp
  • A to T, chromosome 10 at 89,642,007 bp
  • G to A, chromosome 11 at 7,199,919 bp
  • T to A, chromosome 11 at 68,904,458 bp
  • T to A, chromosome 11 at 74,155,571 bp
  • T to A, chromosome 11 at 85,017,718 bp
  • A to G, chromosome 11 at 96,770,709 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to A, chromosome 11 at 102,359,077 bp
  • A to G, chromosome 11 at 102,436,192 bp
  • T to A, chromosome 11 at 121,222,269 bp
  • CGGGGCTCGGGGGC to CGGGGC, chromosome 12 at 8,952,308 bp
  • A to G, chromosome 12 at 51,629,385 bp
  • C to T, chromosome 12 at 84,603,293 bp
  • A to G, chromosome 12 at 118,085,680 bp
  • T to C, chromosome 13 at 17,772,313 bp
  • C to T, chromosome 13 at 67,748,855 bp
  • G to A, chromosome 13 at 113,616,847 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • T to C, chromosome 14 at 61,192,400 bp
  • T to A, chromosome 14 at 101,462,833 bp
  • G to A, chromosome 15 at 8,260,690 bp
  • A to T, chromosome 15 at 28,229,651 bp
  • T to C, chromosome 15 at 82,689,811 bp
  • G to T, chromosome 16 at 13,130,164 bp
  • C to T, chromosome 16 at 55,866,471 bp
  • C to T, chromosome 17 at 35,161,047 bp
  • G to T, chromosome 18 at 33,203,625 bp
  • T to A, chromosome 18 at 42,327,210 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5308 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042891-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.