Strain Name:
C57BL/6J-MtgxR5309Btlr/Mmmh
Stock Number:
042892-MU
Citation ID:
RRID:MMRRC_042892-MU
Other Names:
R5309 (G1), C57BL/6J-MtgxR5309Btlr
Major Collection:

Gene Information

Utrn
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22288
VEGA: 10
Homologene: 21398
Cdh3
Name: cadherin 3
Synonyms: Cadp, Pcad, P-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12560
HGNC: HGNC:1762
Homologene: 20425
Ank2
Name: ankyrin 2, brain
Synonyms: Ankyrin-2, Gm4392, Ank-2, ankyrin B, Ankyrin-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 109676
HGNC: HGNC:493
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77683
Homologene: 11698
Vps35l
Name: VPS35 endosomal protein sorting factor like
Synonyms: Vsp35l, 9030624J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71517
Homologene: 10659
Ipo11
Name: importin 11
Synonyms: 1700081H05Rik, Ranbp11, E330021B14Rik, 2510001A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 76582
Homologene: 7089
Osbpl8
Name: oxysterol binding protein-like 8
Synonyms: ORP-8, D330025H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237542
VEGA: 10
Homologene: 68813
Larp1
Name: La ribonucleoprotein domain family, member 1
Synonyms: 1810024J12Rik, Larp, 3110040D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 73158
Homologene: 9089
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: D630048A14Rik, Ki67, Ki-67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Supt16
Name: SPT16, facilitates chromatin remodeling subunit
Synonyms: Fact140, Supt16h, Spt16, Cdc68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 114741
VEGA: 14
Homologene: 5207
Gfm2
Name: G elongation factor, mitochondrial 2
Synonyms: 6530419G12Rik, MST027, A930009M04Rik, EFG2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 320806
Homologene: 6238
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Cul5
Name: cullin 5
Synonyms: VACM-1, C330021I08Rik, 4921514I20Rik, 8430423K24Rik, C030032G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 75717
HGNC: HGNC:2556
Homologene: 2597
Dnaaf5
Name: dynein, axonemal assembly factor 5
Synonyms: Heatr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 433956
Homologene: 41198
Klc1
Name: kinesin light chain 1
Synonyms: Kns2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 16593
HGNC: HGNC:6387
Homologene: 4056
Osbpl9
Name: oxysterol binding protein-like 9
Synonyms: ORP-9, 2600011I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100273
Homologene: 69380
Shoc2
Name: Shoc2, leucine rich repeat scaffold protein
Synonyms: soc-2 (suppressor of clear) homolog (C. elegans), Sur-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 56392
VEGA: 19
Homologene: 7219
Egfr
Name: epidermal growth factor receptor
Synonyms: Errb1, Wa5, 9030024J15Rik, Erbb, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 13649
HGNC: HGNC:3236
Homologene: 74545
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 319262
Homologene: 14127
Lman1l
Name: lectin, mannose-binding 1 like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235416
HGNC: HGNC:6632
Homologene: 11047
Psmd2
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms: Tex190, 9430095H01Rik, TEG-190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 21762
HGNC: HGNC:9559
Homologene: 2101
Srpk2
Name: serine/arginine-rich protein specific kinase 2
Synonyms: mSRPK2, WBP6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20817
Homologene: 101663
Zfp949
Name: zinc finger protein 949
Synonyms: 4930422I07Rik, Nczf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 71640
Homologene: 129930
Nup160
Name: nucleoporin 160
Synonyms: Gtl1-13, 2810011M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 59015
Homologene: 32509
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Sema4a
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Synonyms: Semab, SemB, SemB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 20351
Homologene: 8425
Sfrp2
Name: secreted frizzled-related protein 2
Synonyms: Sdf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 20319
Homologene: 56438
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: PTPL1, PTP-BL, Ptpri
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217463
VEGA: 12
Homologene: 41011
Mmp9
Name: matrix metallopeptidase 9
Synonyms: Gel B, MMP-9, Clg4b, B/MMP9, Gelatinase B, gelatinase B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17395
HGNC: HGNC:7176
Homologene: 3659
Vmn2r103
Name: vomeronasal 2, receptor 103
Synonyms: EG627636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627636
Homologene: 115024
Cntnap5c
Name: contactin associated protein-like 5C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 620292
VEGA: 17
Homologene: 128806
Myog
Name: myogenin
Synonyms: bHLHc3, myo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17928
HGNC: HGNC:7612
Homologene: 1854
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 229003
Homologene: 14118
Exoc7
Name: exocyst complex component 7
Synonyms: 70kDa, sec70, Exo70
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 53413
Homologene: 41019
Abca15
Name: ATP-binding cassette, sub-family A (ABC1), member 15
Synonyms: 4930500I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 320631
Homologene: 87255
Vmn2r15
Name: vomeronasal 2, receptor 15
Synonyms: EG211223
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 211223
Homologene: 129606
Vmn1r170
Name: vomeronasal 1 receptor 170
Synonyms: Gm5999
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 546944
Homologene: 128341
Slc34a2
Name: solute carrier family 34 (sodium phosphate), member 2
Synonyms: Npt2b, D5Ertd227e, type IIb Na/Picotransporter, NaPi-2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20531
Homologene: 2297
Cyp2j6
Name: cytochrome P450, family 2, subfamily j, polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13110
HGNC: HGNC:2634
Homologene: 68091
Abcg3
Name: ATP binding cassette subfamily G member 3
Synonyms: Mxr2, Abcp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 27405
Homologene: 86845
Adgrg3
Name: adhesion G protein-coupled receptor G3
Synonyms: A030001G24Rik, Gpr97, Pb99
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 54672
Homologene: 18129
Ppp4r4
Name: protein phosphatase 4, regulatory subunit 4
Synonyms: 8430415E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74521
Homologene: 12571
Cwh43
Name: cell wall biogenesis 43 C-terminal homolog
Synonyms: C130090K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231293
Homologene: 5474
Olfr1277
Name: olfactory receptor 1277
Synonyms: GA_x6K02T2Q125-72321818-72320907, MOR248-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258391
Homologene: 105176
Nfil3
Name: nuclear factor, interleukin 3, regulated
Synonyms: E4BP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 18030
HGNC: HGNC:7787
Homologene: 3928
Fam118a
Name: family with sequence similarity 118, member A
Synonyms: 3110048E14Rik, C230014M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 73225
VEGA: 15
HGNC: HGNC:1313
Homologene: 9911
Tmem45a2
Name: transmembrane protein 45A2
Synonyms: 2310005G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 69457
VEGA: 16
Homologene: 131266
Proz
Name: protein Z, vitamin K-dependent plasma glycoprotein
Synonyms: 1300015B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66901
HGNC: HGNC:9460
Homologene: 2890
Spg21
Name: SPG21, maspardin
Synonyms: BM-019, GL010, ACP33, D9Wsu18e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 27965
VEGA: 9
Homologene: 9603
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 639774
Homologene: 106613
Olfr792
Name: olfactory receptor 792
Synonyms: MOR108-2, GA_x6K02T2PULF-11219415-11220350
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258150
Homologene: 133881
Gnal
Name: guanine nucleotide binding protein, alpha stimulating, olfactory type
Synonyms: G alpha 10, Galphaolf, Gna10, 9630020G10Rik, 2610011C15Rik, Golf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 14680
VEGA: 18
HGNC: HGNC:4388
Homologene: 68222
Olfr1284
Name: olfactory receptor 1284
Synonyms: GA_x6K02T2Q125-72430580-72431515, MOR245-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258379
Homologene: 74059
Ccdc173
Name: coiled-coil domain containing 173
Synonyms: 4930578N16Rik, 4930525K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75051
Homologene: 18989
Adamtsl5
Name: ADAMTS-like 5
Synonyms: Thsd6, 2010109H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66548
Homologene: 19803
A630073D07Rik
Name: RIKEN cDNA A630073D07 gene
Synonyms: LOC381819
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 381819
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Slc10a6
Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 6
Synonyms: 8430417G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 75750
Homologene: 12620
Olfr790
Name: olfactory receptor 790
Synonyms: GA_x6K02T2PULF-11179777-11180721, MOR112-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258935
Homologene: 74101
Syf2
Name: SYF2 homolog, RNA splicing factor (S. cerevisiae)
Synonyms: mp29, p29, 1110018L13Rik, Cbpin, Ntc31, D4Bwg1551e, Gcipip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 68592
Homologene: 5993
Fbxo10
Name: F-box protein 10
Synonyms: LOC269529, FBX10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 269529
Homologene: 19544
Rnf5
Name: ring finger protein 5
Synonyms: 2410131O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 54197
Homologene: 56004
Ighv1-74
Name: immunoglobulin heavy variable V1-74
Synonyms: Gm16710
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 100775173
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 134,290,326 bp
  • A to G, chromosome 2 at 24,884,195 bp
  • T to C, chromosome 2 at 69,787,258 bp
  • G to T, chromosome 2 at 90,732,832 bp
  • T to G, chromosome 2 at 111,270,310 bp
  • T to C, chromosome 2 at 111,379,834 bp
  • T to A, chromosome 2 at 164,950,795 bp
  • T to A, chromosome 2 at 181,234,846 bp
  • A to G, chromosome 3 at 83,769,401 bp
  • G to A, chromosome 3 at 88,437,036 bp
  • T to C, chromosome 3 at 126,959,768 bp
  • A to G, chromosome 3 at 138,958,628 bp
  • A to G, chromosome 4 at 43,003,019 bp
  • A to T, chromosome 4 at 45,042,036 bp
  • T to A, chromosome 4 at 96,535,556 bp
  • A to G, chromosome 4 at 109,066,155 bp
  • C to A, chromosome 4 at 111,950,193 bp
  • A to G, chromosome 4 at 134,936,069 bp
  • T to C, chromosome 5 at 23,525,718 bp
  • A to G, chromosome 5 at 53,069,488 bp
  • A to G, chromosome 5 at 73,416,767 bp
  • G to A, chromosome 5 at 103,541,053 bp
  • A to T, chromosome 5 at 103,609,092 bp
  • A to C, chromosome 5 at 104,936,599 bp
  • T to A, chromosome 5 at 109,293,090 bp
  • T to A, chromosome 5 at 139,152,862 bp
  • T to C, chromosome 6 at 132,626,577 bp
  • T to A, chromosome 7 at 23,606,455 bp
  • T to C, chromosome 7 at 118,813,576 bp
  • T to C, chromosome 7 at 120,345,369 bp
  • A to T, chromosome 7 at 135,700,830 bp
  • T to C, chromosome 8 at 13,061,049 bp
  • G to A, chromosome 8 at 95,039,864 bp
  • A to G, chromosome 8 at 106,539,020 bp
  • A to G, chromosome 9 at 53,633,735 bp
  • A to T, chromosome 9 at 57,611,077 bp
  • A to G, chromosome 9 at 65,468,802 bp
  • A to C, chromosome 9 at 88,567,183 bp
  • A to T, chromosome 10 at 12,727,769 bp
  • A to T, chromosome 10 at 80,345,148 bp
  • T to A, chromosome 10 at 111,270,557 bp
  • T to A, chromosome 10 at 129,501,514 bp
  • A to C, chromosome 10 at 129,541,265 bp
  • G to A, chromosome 11 at 16,911,703 bp
  • T to C, chromosome 11 at 58,050,808 bp
  • C to A, chromosome 11 at 116,305,027 bp
  • C to T, chromosome 12 at 35,144,325 bp
  • T to A, chromosome 12 at 103,606,888 bp
  • A to G, chromosome 12 at 111,795,621 bp
  • A to G, chromosome 12 at 115,802,881 bp
  • A to T, chromosome 13 at 52,967,620 bp
  • G to A, chromosome 13 at 97,163,151 bp
  • T to C, chromosome 13 at 106,833,973 bp
  • T to C, chromosome 14 at 52,162,698 bp
  • C to T, chromosome 15 at 85,050,755 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • T to C, chromosome 16 at 20,660,420 bp
  • T to C, chromosome 16 at 57,039,007 bp
  • A to T, chromosome 17 at 19,793,034 bp
  • A to G, chromosome 17 at 34,601,588 bp
  • A to T, chromosome 17 at 58,359,254 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • G to A, chromosome 18 at 67,213,107 bp
  • T to C, chromosome 19 at 53,987,733 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5309 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042892-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.