Strain Name:
C57BL/6J-MtgxR5315Btlr/Mmmh
Stock Number:
042898-MU
Citation ID:
RRID:MMRRC_042898-MU
Other Names:
R5315 (G1), C57BL/6J-MtgxR5315Btlr
Major Collection:

Gene Information

Epha4
Name: Eph receptor A4
Synonyms: Cek8, 2900005C20Rik, rb, Tyro1, Sek1, Hek8, Sek
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 2900036C23Rik, 6030449K19Rik, 2410152H17Rik, 2810413I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66315
Homologene: 10778
Mtf2
Name: metal response element binding transcription factor 2
Synonyms: Pcl2, C76717, 9230112N11Rik, M96
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17765
Homologene: 7207
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: Mms19, 2610042O15Rik, C86341, Mms19l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72199
Homologene: 41480
Slc9a8
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8
Synonyms: NHE8, 6430709P13Rik, 1200006P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77031
Homologene: 75041
Krit1
Name: KRIT1, ankyrin repeat containing
Synonyms: Krit1, A630036P20Rik, Krit1A, 2010007K12Rik, Krit1B, Ccm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 79264
HGNC: HGNC:1573
Homologene: 12746
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: b2b1035Clo, 5730509K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231214
Homologene: 18159
Mepce
Name: methylphosphate capping enzyme
Synonyms: D5Wsu46e, Bcdin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231803
Homologene: 32447
Tbc1d14
Name: TBC1 domain family, member 14
Synonyms: D5Ertd110e, 2810413P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100855
Homologene: 10809
Ass1
Name: argininosuccinate synthetase 1
Synonyms: fold, ASS, Ass-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11898
HGNC: HGNC:758
Homologene: 6899
Dag1
Name: dystroglycan 1
Synonyms: D9Wsu13e, DG, dystrophin associated glycoprotein 1, beta-dystroglycan, alpha-dystroglycan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13138
HGNC: HGNC:2666
Homologene: 3234
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Katnal2
Name: katanin p60 subunit A-like 2
Synonyms: 4933439B08Rik, 3110023G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71206
Homologene: 41725
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99412
HGNC: HGNC:4425
Homologene: 3300
AU041133
Name: expressed sequence AU041133
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216177
Homologene: 87560
Siglecf
Name: sialic acid binding Ig-like lectin F
Synonyms: mSiglec-F, Siglec5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233186
Homologene: 50482
A830010M20Rik
Name: RIKEN cDNA A830010M20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231570
VEGA: 5
Homologene: 128318
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: Dnahc17, Dnahcl1, 2810003K23Rik, LOC382552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Olfr502
Name: olfactory receptor 502
Synonyms: MOR204-8, GA_x6K02T2PBJ9-10853935-10852991
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258734
Homologene: 72021
Slc4a1
Name: solute carrier family 4 (anion exchanger), member 1
Synonyms: CD233, Ae1, Empb3, band 3, erythrocyte membrane protein band 3, l11Jus51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20533
Homologene: 133556
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Rnf224
Name: ring finger protein 224
Synonyms: Gm757, LOC329360
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329360
Homologene: 121711
Pitpnm2
Name: phosphatidylinositol transfer protein, membrane-associated 2
Synonyms: Rdgb2, RDGBA2, NIR3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19679
Homologene: 7915
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Eri2
Name: exoribonuclease 2
Synonyms: Exod1, 4933424N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71151
Homologene: 12383
Sh2d2a
Name: SH2 domain containing 2A
Synonyms: Rlk/Itk-binding protein, RIBP, Lad, Lck-associated adapter protein, TSAd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 27371
Homologene: 2958
Plin5
Name: perilipin 5
Synonyms: MLDP, Lsdp5, 2310076L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66968
Homologene: 41654
Grsf1
Name: G-rich RNA sequence binding factor 1
Synonyms: D5Wsu31e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231413
HGNC: HGNC:4610
Homologene: 20482
Olfr284
Name: olfactory receptor 284
Synonyms: GA_x6K02T2NBG7-5395976-5396893, MOR160-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 258278
Homologene: 74250
Nt5c2
Name: 5'-nucleotidase, cytosolic II
Synonyms: NT5B, cN-II, PNT5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76952
HGNC: HGNC:8022
Homologene: 8158
Appl2
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Synonyms: Dip3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216190
Homologene: 10046
Rrp8
Name: ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Synonyms: 2900001K19Rik, 1500003O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101867
Homologene: 5621
Zfp850
Name: zinc finger protein 850
Synonyms: Gm4636, C130069I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043772
Tmem106b
Name: transmembrane protein 106B
Synonyms: 6430519M21Rik, 5830455K21Rik, 2310036D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71900
Homologene: 56806
Rab3a
Name: RAB3A, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19339
HGNC: HGNC:9777
Homologene: 20629
Ace3
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 3
Synonyms: EG217246
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217246
Homologene: 80337
Sftpb
Name: surfactant associated protein B
Synonyms: SF-B, Sftp-3, Sftp3, SP-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20388
Homologene: 456
Cflar
Name: CASP8 and FADD-like apoptosis regulator
Synonyms: Casper, c-Flip, Cash, A430105C05Rik, 2310024N18Rik, Flip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12633
HGNC: HGNC:1876
Homologene: 7652
S100a7l2
Name: S100 calcium binding protein A7 like 2
Synonyms: LOC229550, 9130204L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229550
Homologene: 135857
Bex6
Name: brain expressed family member 6
Synonyms: B020003O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 328660
Igkv6-15
Name: immunoglobulin kappa variable 6-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108022
Gm11677
Name: predicted gene 11677
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 629967
Anxa11os
Name: annexin A11, opposite strand
Synonyms: Gm9872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 58,753,802 bp
  • C to T, chromosome 1 at 77,388,472 bp
  • T to A, chromosome 2 at 25,235,859 bp
  • A to T, chromosome 2 at 31,492,329 bp
  • A to G, chromosome 2 at 32,303,761 bp
  • A to G, chromosome 2 at 167,474,166 bp
  • C to A, chromosome 3 at 64,116,956 bp
  • A to C, chromosome 3 at 87,847,669 bp
  • G to A, chromosome 3 at 91,090,330 bp
  • A to G, chromosome 5 at 3,811,266 bp
  • T to C, chromosome 5 at 36,507,588 bp
  • A to G, chromosome 5 at 43,720,433 bp
  • G to A, chromosome 5 at 88,673,775 bp
  • A to T, chromosome 5 at 107,508,237 bp
  • A to G, chromosome 5 at 108,093,395 bp
  • A to T, chromosome 5 at 124,121,933 bp
  • A to C, chromosome 5 at 137,782,693 bp
  • T to G, chromosome 6 at 13,081,560 bp
  • A to T, chromosome 6 at 31,449,539 bp
  • A to C, chromosome 6 at 70,406,973 bp
  • G to T, chromosome 6 at 72,306,892 bp
  • T to C, chromosome 7 at 27,990,318 bp
  • T to C, chromosome 7 at 43,355,108 bp
  • T to C, chromosome 7 at 105,734,000 bp
  • A to G, chromosome 7 at 108,523,890 bp
  • T to C, chromosome 7 at 119,786,018 bp
  • T to C, chromosome 8 at 70,755,922 bp
  • A to G, chromosome 9 at 108,209,117 bp
  • T to G, chromosome 10 at 82,151,672 bp
  • A to G, chromosome 10 at 83,614,152 bp
  • T to A, chromosome 11 at 102,358,254 bp
  • T to C, chromosome 11 at 105,994,921 bp
  • C to T, chromosome 11 at 111,724,711 bp
  • G to A, chromosome 11 at 118,127,283 bp
  • T to C, chromosome 14 at 25,870,020 bp
  • T to C, chromosome 15 at 9,596,691 bp
  • G to A, chromosome 15 at 98,340,365 bp
  • A to G, chromosome 16 at 32,186,493 bp
  • A to G, chromosome 16 at 56,180,526 bp
  • C to A, chromosome 17 at 56,114,066 bp
  • A to T, chromosome 18 at 77,012,009 bp
  • C to T, chromosome 19 at 41,954,762 bp
  • T to C, chromosome 19 at 46,892,243 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5315 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042898-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.