Strain Name:
B6.129S-Cd74tm2Liz/Mmnc
Stock Number:
000429-UNC
Citation ID:
RRID:MMRRC_000429-UNC

Strain Information

Cd74tm2Liz
Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); targeted mutation 2, Elizabeth K Bikoff
Synonyms: 31
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout
Cd74
Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Synonyms: Ii, CLIP
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Knockout
NCBI: 16149
VEGA: 18
HGNC: HGNC:1697
Homologene: 3209
Genetic Alterations
Approximately 6 kb of Ii chain genomic homology is contained within the 7 kb Sacl-EcoRI fragment of pBSlilORI. The mutation wascreated by deleting the 1 kb Afllll-Bstl1071 fragment encompassing exon 6b and flanking intronic sequence. Deletion of exon 6b results in expression of the p31 isoform but not p41.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    CCE/EK.CCE derived from 129S8/SvEv-Gpi1c
    Phenotype
    Spleen cells from these mice appear indistinguishable from wild type with respect to class I I subunit assembly, transport, peptide acquisition, surface expression, and the ability to present intact protein antigens. Moreover, these mutant mice have normal numbers of thymic and peripheral CD4+ T cells, and intact CD4+
    T-dependent proliferative responses towards a soluble antigen.
    Mammalian Phenotype Terms
    Allelic Composition: Cd74tm2Liz/Cd74tm2Liz (Genetic Background: involves: 129S/SvEv * C57BL/6 )

    Allelic Composition: Cd74tm2Liz/Cd74tm1Liz (Genetic Background: involves: 129S/SvEv * C57BL/6 )

    MeSH Terms
    • Animals
    • Antigen Presentation
    • Antigens, Differentiation, B-Lymphocyte/genetics
    • Antigens, Differentiation, B-Lymphocyte/physiology
    • Base Sequence
    • CD4-Positive T-Lymphocytes/physiology
    • Exons
    • Histocompatibility Antigens Class II/genetics
    • Histocompatibility Antigens Class II/metabolism
    • Histocompatibility Antigens Class II/physiology
    • Lymphocyte Activation
    • Mice
    • Mice, Inbred BALB C
    • Mice, Inbred C57BL
    • Molecular Sequence Data
    • Mutation
    • Amino Acid Sequence
    • Antigens, CD/biosynthesis
    • Antigens, CD/classification
    • Antigens, CD/pharmacology
    • Antigens, Differentiation, B-Lymphocyte/biosynthesis
    • Antigens, Differentiation, B-Lymphocyte/classification
    • Antigens, Differentiation, B-Lymphocyte/pharmacology
    • CD4-Positive T-Lymphocytes/drug effects
    • CD48 Antigen
    • Histocompatibility Antigens Class II/biosynthesis
    • Histocompatibility Antigens Class II/classification
    • Histocompatibility Antigens Class II/pharmacology
    • Lymphocyte Activation/drug effects
    • Mice, Mutant Strains
    • Antigens, Differentiation, B-Lymphocyte/metabolism
    • Cathepsin L
    • Cathepsins/metabolism
    • Cysteine Endopeptidases/metabolism
    • Endocytosis
    • Intracellular Fluid/metabolism
    • Mice, Knockout
    • Molecular Chaperones/genetics
    • Molecular Chaperones/metabolism
    • Protein Isoforms/genetics
    • Protein Isoforms/metabolism
    Strain Development
    To generate chimeras, recombinant ES cell clones were injected into C57BU6 blastocysts.
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    Donor
    Elizabeth Bikoff, Ph.D., Harvard University
    Primary Reference
    • Takaesu NT, Lower JA, Robertson EJ, Bikoff EK. Major histocompatibility class II peptide occupancy, antigen presentation, and CD4+ T cell function in mice lacking the p41 isoform of invariant chain. Immunity. 1995 Sep;3(3):385-96. (Medline PMID: 7553002)
    • Takaesu NT, Lower JA, Yelon D, Robertson EJ, Bikoff EK. In vivo functions mediated by the p41 isoform of the MHC class II-associated invariant chain. J Immunol. 1997 Jan 1;158(1):187-99. (Medline PMID: 8977190)
    • Lennon-Dumenil AM, Roberts RA, Valentijn K, Driessen C, Overkleeft HS, Erickson A, Peters PJ, Bikoff E, Ploegh HL, Wolf Bryant P. The p41 isoform of invariant chain is a chaperone for cathepsin L. EMBO J. 2001 Aug 1;20(15):4055-64. (Medline PMID: 11483509)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000429-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.