Strain Name:
C57BL/6J-MtgxR5321Btlr/Mmmh
Stock Number:
042904-MU
Citation ID:
RRID:MMRRC_042904-MU
Other Names:
R5321 (G1), C57BL/6J-MtgxR5321Btlr
Major Collection:

Gene Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1-D, NFAT1, NFATp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Btrc
Name: beta-transducin repeat containing protein
Synonyms: Fbw1a, Beta-Trcp1, SCF b-TRCP, beta-TrCP, Slimb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67937
Homologene: 8104
Cryzl1
Name: crystallin, zeta (quinone reductase)-like 1
Synonyms: 2210407J23Rik, 2410006O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 66609
HGNC: HGNC:2420
Homologene: 3749
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: 9330175N20Rik, RPC1, RPC155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218832
VEGA: 14
Homologene: 5124
Utp18
Name: UTP18 small subunit processome component
Synonyms: Wdr50, 6230425C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217109
Homologene: 41087
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 64898
Homologene: 8769
Dzip3
Name: DAZ interacting protein 3, zinc finger
Synonyms: 6430549P11Rik, 2A-HUB, 2310047C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224170
Homologene: 8771
Exosc1
Name: exosome component 1
Synonyms: 2610104C07Rik, 2610035C18Rik, 2610312F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 66583
VEGA: 19
Homologene: 9359
Patl1
Name: protein associated with topoisomerase II homolog 1 (yeast)
Synonyms: Pat1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 225929
VEGA: 19
Homologene: 82269
Uxs1
Name: UDP-glucuronate decarboxylase 1
Synonyms: 1600025I13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67883
Homologene: 41609
Flywch1
Name: FLYWCH-type zinc finger 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224613
VEGA: 17
Homologene: 72130
Trpv2
Name: transient receptor potential cation channel, subfamily V, member 2
Synonyms: VRL-1, Vrl1, OTRPC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 22368
Homologene: 7993
Vmn2r10
Name: vomeronasal 2, receptor 10
Synonyms: VR16, V2r16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22307
Homologene: 129606
Vmn1r24
Name: vomeronasal 1 receptor 24
Synonyms: V1rc18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171191
Homologene: 134024
Tdrd12
Name: tudor domain containing 12
Synonyms: 2410004F06Rik, ecat8, 2410070K17Rik, G1-476-14, EG434165, repro23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71981
Homologene: 87053
Vmn1r20
Name: vomeronasal 1 receptor 20
Synonyms: Gm5569
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 434017
Homologene: 79462
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 665033
Homologene: 122792
Abca5
Name: ATP-binding cassette, sub-family A (ABC1), member 5
Synonyms: ABC13, B930033A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217265
HGNC: HGNC:35
Homologene: 10263
Dlec1
Name: deleted in lung and esophageal cancer 1
Synonyms: D630005C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320256
HGNC: HGNC:2899
Homologene: 84733
Phtf1
Name: putative homeodomain transcription factor 1
Synonyms: Phft
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18685
HGNC: HGNC:8939
Homologene: 4817
Tpte
Name: transmembrane phosphatase with tensin homology
Synonyms: Pten2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234129
Homologene: 50411
Slc2a4
Name: solute carrier family 2 (facilitated glucose transporter), member 4
Synonyms: Glut4, twgy, Glut-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20528
Homologene: 74381
Slc15a5
Name: solute carrier family 15, member 5
Synonyms: 9830102E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 277898
Homologene: 65333
Vmn1r5
Name: vomeronasal 1 receptor 5
Synonyms: V1rc19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171192
Homologene: 128340
Endou
Name: endonuclease, polyU-specific
Synonyms: Tcl-30, Pp11r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 19011
VEGA: 15
Homologene: 48394
Zfp119a
Name: zinc finger protein 119a
Synonyms: Zfp119, Mzf13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 104349
Slc17a2
Name: solute carrier family 17 (sodium phosphate), member 2
Synonyms: NPT3, C730032N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218103
Homologene: 4260
Gm340
Name: predicted gene 340
Synonyms: LOC381224
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
Olfr629
Name: olfactory receptor 629
Synonyms: MOR26-2, GA_x6K02T2PBJ9-6466772-6465828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258818
Homologene: 105159
Gm14410
Name: predicted gene 14410
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 100043403
Tdrkh
Name: tudor and KH domain containing protein
Synonyms: Tdrd2, 2700091C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 72634
Homologene: 4999
Ugdh
Name: UDP-glucose dehydrogenase
Synonyms: Udpgdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22235
Homologene: 2520
Scrt1
Name: scratch family zinc finger 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 170729
VEGA: 15
Homologene: 113534
4930449A18Rik
Name: RIKEN cDNA 4930449A18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
Mgam2-ps
Name: maltase-glucoamylase 2, pseudogene
Synonyms: Gm7254
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 638904
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,805,645 bp
  • A to G, chromosome 2 at 168,567,304 bp
  • G to A, chromosome 2 at 177,193,505 bp
  • C to T, chromosome 3 at 59,846,696 bp
  • C to A, chromosome 3 at 94,425,658 bp
  • C to T, chromosome 3 at 104,003,511 bp
  • A to G, chromosome 5 at 65,422,729 bp
  • G to T, chromosome 5 at 108,995,639 bp
  • T to C, chromosome 6 at 40,833,833 bp
  • T to C, chromosome 6 at 56,985,607 bp
  • C to T, chromosome 6 at 57,432,442 bp
  • A to T, chromosome 6 at 57,956,197 bp
  • G to T, chromosome 6 at 137,987,438 bp
  • A to C, chromosome 7 at 35,478,094 bp
  • T to C, chromosome 7 at 103,740,655 bp
  • A to T, chromosome 8 at 22,297,203 bp
  • A to G, chromosome 8 at 70,487,215 bp
  • A to T, chromosome 9 at 105,928,465 bp
  • T to A, chromosome 9 at 119,112,601 bp
  • T to A, chromosome 11 at 62,584,571 bp
  • G to A, chromosome 11 at 69,946,065 bp
  • A to G, chromosome 11 at 93,866,434 bp
  • A to G, chromosome 11 at 110,327,825 bp
  • A to T, chromosome 13 at 23,812,631 bp
  • T to C, chromosome 14 at 24,454,941 bp
  • T to C, chromosome 15 at 76,519,170 bp
  • A to G, chromosome 15 at 97,721,032 bp
  • T to G, chromosome 16 at 48,957,675 bp
  • T to C, chromosome 16 at 91,707,230 bp
  • C to G, chromosome 17 at 23,756,651 bp
  • A to T, chromosome 17 at 55,865,595 bp
  • G to A, chromosome 17 at 71,246,858 bp
  • A to G, chromosome 19 at 11,921,421 bp
  • G to T, chromosome 19 at 41,585,204 bp
  • A to T, chromosome 19 at 41,924,060 bp
  • G to A, chromosome 19 at 45,507,758 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5321 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042904-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.