Strain Name:
C57BL/6J-MtgxR5324Btlr/Mmmh
Stock Number:
042907-MU
Citation ID:
RRID:MMRRC_042907-MU
Other Names:
R5324 (G1), C57BL/6J-MtgxR5324Btlr
Major Collection:

Gene Information

Akap10
Name: A kinase (PRKA) anchor protein 10
Synonyms: D-AKAP2, B130049N18Rik, 1500031L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56697
HGNC: HGNC:368
Homologene: 32452
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: Spna2, 2610027H02Rik, Spna-2, alpha-fodrin, alphaII-spectrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20740
Homologene: 2353
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Nmp4, Ciz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269800
Homologene: 15849
Psma2
Name: proteasome subunit alpha 2
Synonyms: Lmpc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19166
VEGA: 13
HGNC: HGNC:9531
Homologene: 2081
Fmn2
Name: formin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 54418
Homologene: 137334
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214133
Homologene: 49498
Cbl
Name: Casitas B-lineage lymphoma
Synonyms: c-Cbl, 4732447J05Rik, Cbl-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12402
HGNC: HGNC:1541
Homologene: 3802
Serpini1
Name: serine (or cysteine) peptidase inhibitor, clade I, member 1
Synonyms: Ns, Spi17, Neuroserpin, PI12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20713
HGNC: HGNC:8943
Homologene: 21045
N6amt1
Name: N-6 adenine-specific DNA methyltransferase 1 (putative)
Synonyms: Pred28, Hemk2, 5830445C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67768
Homologene: 5637
Bmp2
Name: bone morphogenetic protein 2
Synonyms: Bmp2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12156
HGNC: HGNC:1069
Homologene: 926
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Pabpc1
Name: poly(A) binding protein, cytoplasmic 1
Synonyms: Pabp1, Pabpl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18458
HGNC: HGNC:8554
Homologene: 37638
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: cytochrome P450 subfamily I, polypeptide 1, P450-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Hbp1
Name: high mobility group box transcription factor 1
Synonyms: C330012F01Rik, 1700058O05Rik, C86454
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73389
Homologene: 8171
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, A130095G14Rik, 5830472H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, Dnahc2, Dnhd3, 2900022L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Lrguk
Name: leucine-rich repeats and guanylate kinase domain containing
Synonyms: 4921528H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74354
Homologene: 34923
Col14a1
Name: collagen, type XIV, alpha 1
Synonyms: 5730412L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12818
HGNC: HGNC:2191
Homologene: 18741
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214321
Homologene: 86950
Zxdc
Name: ZXD family zinc finger C
Synonyms: B930086F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 80292
Homologene: 82340
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
Olfr805
Name: olfactory receptor 805
Synonyms: MOR110-4, GA_x6K02T2PULF-11402237-11401278
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258548
Plch2
Name: phospholipase C, eta 2
Synonyms: Plcl4, A930027K05Rik, PLCeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269615
Homologene: 85172
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243083
Homologene: 65356
Olfr1151
Name: olfactory receptor 1151
Synonyms: MOR177-9, GA_x6K02T2Q125-49358694-49359620
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258631
Homologene: 36999
Mmrn1
Name: multimerin 1
Synonyms: Emilin4, 4921530G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70945
HGNC: HGNC:7178
Homologene: 49134
Dpm1
Name: dolichol-phosphate (beta-D) mannosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13480
HGNC: HGNC:3005
Homologene: 2865
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Dip2, Kiaa0184-hp, 4931420H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64451
Homologene: 41012
Tcp11
Name: t-complex protein 11
Synonyms: D17Ken1, Tcp-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21463
Homologene: 8433
Olfr598
Name: olfactory receptor 598
Synonyms: GA_x6K02T2PBJ9-6037823-6038782, MOR23-4P, MOR23-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257975
Homologene: 132401
Parp12
Name: poly (ADP-ribose) polymerase family, member 12
Synonyms: Zc3hdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243771
Homologene: 11236
Rpe65
Name: retinal pigment epithelium 65
Synonyms: rd12, A930029L06Rik, Mord1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19892
Homologene: 20108
Olfr682-ps1
Name: olfactory receptor 682, pseudogene 1
Synonyms: Olfr217-ps1, GA_x6K02T2PBJ9-7755919-7755070, MOR40-19_p, GA_x6K02SYW8DF-188-1037, MOR40-6P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258187
Tmem71
Name: transmembrane protein 71
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 213068
VEGA: 15
Homologene: 51638
Ap5b1
Name: adaptor-related protein complex 5, beta 1 subunit
Synonyms: Gm962
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381201
VEGA: 19
Homologene: 16299
9130019O22Rik
Name: RIKEN cDNA 9130019O22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78921
Homologene: 118005
Rdh16
Name: retinol dehydrogenase 16
Synonyms: CRAD1, Rdh6, cis-retinol/androgen dehydrogenase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19683
Homologene: 129514
Mroh9
Name: maestro heat-like repeat family member 9
Synonyms: 4921528O07Rik, Armc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78258
Homologene: 123521
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 163,060,760 bp
  • G to A, chromosome 1 at 174,608,880 bp
  • A to T, chromosome 2 at 29,993,724 bp
  • A to T, chromosome 2 at 87,857,696 bp
  • C to T, chromosome 2 at 133,561,359 bp
  • C to T, chromosome 2 at 168,210,571 bp
  • T to C, chromosome 3 at 75,640,294 bp
  • T to C, chromosome 3 at 133,485,913 bp
  • A to G, chromosome 3 at 159,604,404 bp
  • T to A, chromosome 4 at 154,984,534 bp
  • A to G, chromosome 5 at 72,435,257 bp
  • T to A, chromosome 5 at 86,556,978 bp
  • T to C, chromosome 6 at 34,073,797 bp
  • A to T, chromosome 6 at 39,102,612 bp
  • A to T, chromosome 6 at 60,976,586 bp
  • T to A, chromosome 6 at 90,373,800 bp
  • G to A, chromosome 6 at 125,031,423 bp
  • T to A, chromosome 7 at 103,329,050 bp
  • C to T, chromosome 7 at 105,126,777 bp
  • G to T, chromosome 7 at 127,384,907 bp
  • A to T, chromosome 9 at 44,154,254 bp
  • A to G, chromosome 9 at 57,702,369 bp
  • A to T, chromosome 9 at 121,727,346 bp
  • T to C, chromosome 10 at 76,296,393 bp
  • G to A, chromosome 10 at 127,801,267 bp
  • T to A, chromosome 10 at 129,722,945 bp
  • C to T, chromosome 11 at 61,916,189 bp
  • T to A, chromosome 11 at 69,457,993 bp
  • T to C, chromosome 12 at 31,928,618 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to C, chromosome 13 at 14,625,217 bp
  • A to G, chromosome 15 at 36,600,625 bp
  • A to T, chromosome 15 at 55,338,445 bp
  • C to T, chromosome 15 at 66,555,214 bp
  • A to G, chromosome 16 at 87,354,353 bp
  • G to A, chromosome 17 at 28,067,863 bp
  • A to G, chromosome 18 at 77,962,445 bp
  • G to A, chromosome 19 at 5,569,835 bp
  • T to C, chromosome 19 at 25,163,094 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042907-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.