Strain Name:
C57BL/6J-MtgxR5325Btlr/Mmmh
Stock Number:
042908-MU
Citation ID:
RRID:MMRRC_042908-MU
Other Names:
R5325 (G1), C57BL/6J-MtgxR5325Btlr
Major Collection:

Gene Information

Tnpo3
Name: transportin 3
Synonyms: C430013M08Rik, D6Ertd313e, 5730544L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320938
Homologene: 40848
Cep83
Name: centrosomal protein 83
Synonyms: 5730513H21Rik, 4921537D05Rik, Ccdc41, 2600001G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 77048
VEGA: 10
Homologene: 9396
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, A930013E17Rik, Zap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 56531
Homologene: 87707
Sin3b
Name: transcriptional regulator, SIN3B (yeast)
Synonyms: 2810430C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 20467
Homologene: 81810
Odf2
Name: outer dense fiber of sperm tails 2
Synonyms: MMTEST29, cenexin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18286
HGNC: HGNC:8114
Homologene: 1907
Lrch4
Name: leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms: SAP25, LRN, 2810008P14Rik, LRRN4, 2900069C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231798
HGNC: HGNC:6691
Homologene: 20532
Pan2
Name: PAN2 poly(A) specific ribonuclease subunit
Synonyms: 1200014O24Rik, Usp52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 103135
VEGA: 10
Homologene: 5918
Rad50
Name: RAD50 double strand break repair protein
Synonyms: Rad50l, Mrell
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 19360
HGNC: HGNC:9816
Homologene: 38092
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20191
VEGA: 13
Homologene: 37423
Robo2
Name: roundabout guidance receptor 2
Synonyms: 2600013A04Rik, 9430089E08Rik, D230004I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 268902
Homologene: 43188
Smim17
Name: small integral membrane protein 17
Synonyms: Gm16532
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100042450
Homologene: 104897
Grhl3
Name: grainyhead like transcription factor 3
Synonyms: ct, Som, Get1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230824
Homologene: 18864
Ccnb1
Name: cyclin B1
Synonyms: Ccnb1-rs13, Cycb-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 268697
HGNC: HGNC:1579
Homologene: 68982
St14
Name: suppression of tumorigenicity 14 (colon carcinoma)
Synonyms: Tmprss14, Prss14, Epithin, matriptase, MT-SP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19143
Homologene: 7906
Wwtr1
Name: WW domain containing transcription regulator 1
Synonyms: 2610021I22Rik, transcriptional coactivator with PDZ binding motif, 2310058J06Rik, TAZ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 97064
Homologene: 9159
Nudt9
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 9
Synonyms: 1190002C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 74167
HGNC: HGNC:8056
Homologene: 11435
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, Sec3l1, 2810407P21Rik, SEC3, A730011E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69940
Homologene: 41241
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216565
Homologene: 22880
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: Flk2, wmfl, Flt-3, CD135, Flk-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Nr2e1
Name: nuclear receptor subfamily 2, group E, member 1
Synonyms: Nr2e1, Tlx, tailless, Mtll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 21907
HGNC: HGNC:7973
Homologene: 37750
Ddr2
Name: discoidin domain receptor family, member 2
Synonyms: Ntrkr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18214
HGNC: HGNC:2731
Homologene: 68505
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Gpr63
Name: G protein-coupled receptor 63
Synonyms: PSP24beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 81006
Homologene: 12759
Wdr17
Name: WD repeat domain 17
Synonyms: 3010002I12Rik, B230207L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 244484
Homologene: 12460
Fbxo30
Name: F-box protein 30
Synonyms: 1700026A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 71865
Homologene: 12959
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 214321
Homologene: 86950
Evpl
Name: envoplakin
Synonyms: 210kDa protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14027
HGNC: HGNC:3503
Homologene: 1506
Syne4
Name: spectrin repeat containing, nuclear envelope family member 4
Synonyms: 0610012K07Rik, nesprin-4, AI428936
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233066
Homologene: 17716
Ctsj
Name: cathepsin J
Synonyms: Ctsp, CatRLP, CATP, rat gene/Cathepsin L-related protein, Cat P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 26898
VEGA: 13
Homologene: 137246
Noa1
Name: nitric oxide associated 1
Synonyms: 2610024G14Rik, mAtNOS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56412
Homologene: 10518
Spib
Name: Spi-B transcription factor (Spi-1/PU.1 related)
Synonyms: Spi-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 272382
Homologene: 79220
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 636697
Homologene: 41799
Fndc7
Name: fibronectin type III domain containing 7
Synonyms: E230011A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 320181
Homologene: 18551
Osbpl5
Name: oxysterol binding protein-like 5
Synonyms: 1110006M06Rik, Obph1, ORP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 79196
Homologene: 98024
Sytl1
Name: synaptotagmin-like 1
Synonyms: Slp1, PSGL-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 269589
Homologene: 12853
Hrh4
Name: histamine receptor H4
Synonyms: H4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225192
VEGA: 18
Homologene: 11002
Sirpb1a
Name: signal-regulatory protein beta 1A
Synonyms: Sirpb1, 9930027N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 320832
Homologene: 82993
Ccdc186
Name: coiled-coil domain containing 186
Synonyms: A630007B06Rik, 1810028B20Rik, Otg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 213993
Homologene: 9963
H2-M10.2
Name: histocompatibility 2, M region locus 10.2
Synonyms: 4.7H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 333715
Homologene: 117973
Fam98b
Name: family with sequence similarity 98, member B
Synonyms: 2610510H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 68215
Homologene: 72241
Ift88
Name: intraflagellar transport 88
Synonyms: IFT88, polaris, Tg737, Oak Ridge polycystic kidneys, fxo, Tg737Rpw, Ttc10, TgN737Rpw, orpk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21821
Homologene: 4761
Olfr522
Name: olfactory receptor 522
Synonyms: GA_x6K02T2PBJ9-42315125-42314187, MOR103-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258954
Homologene: 62435
Gm7075
Name: predicted gene 7075
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 631906
VEGA: 10
Sgsh
Name: N-sulfoglucosamine sulfohydrolase (sulfamidase)
Synonyms: 4632406A19Rik, sulphamidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 27029
Homologene: 167
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Olfr539
Name: olfactory receptor 539
Synonyms: MOR253-4, GA_x6K02T2PBJ9-42813436-42814368
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258963
Homologene: 79393
Zfp783
Name: zinc finger protein 783
Synonyms: Gm20494, ENSMUSG00000072653, A230106D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232785
Ace3
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 3
Synonyms: EG217246
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217246
Homologene: 80337
Trim12a
Name: tripartite motif-containing 12A
Synonyms: Trim12, 2310043C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 76681
Tspan1
Name: tetraspanin 1
Synonyms: 9030418M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66805
Homologene: 21170
2310040G07Rik
Name: RIKEN cDNA 2310040G07 gene
Synonyms: Gm16528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Mroh9
Name: maestro heat-like repeat family member 9
Synonyms: 4921528O07Rik, Armc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 78258
Homologene: 123521
Olfr908
Name: olfactory receptor 908
Synonyms: GA_x6K02T2PVTD-32216179-32217111, MOR165-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258872
VEGA: 9
Homologene: 74149
BC048644
Name: cDNA sequence BC048644
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 407789
Gm6457
Name: predicted pseudogene 6457
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 623867
VEGA: 18
RP23-357H5.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 163,060,760 bp
  • T to A, chromosome 1 at 170,001,837 bp
  • T to A, chromosome 2 at 29,912,571 bp
  • A to C, chromosome 2 at 117,270,651 bp
  • G to A, chromosome 3 at 15,411,443 bp
  • A to T, chromosome 3 at 57,575,237 bp
  • G to A, chromosome 3 at 108,883,449 bp
  • T to C, chromosome 4 at 25,007,294 bp
  • A to G, chromosome 4 at 43,006,564 bp
  • T to A, chromosome 4 at 116,164,339 bp
  • T to C, chromosome 4 at 133,261,071 bp
  • T to A, chromosome 4 at 135,559,104 bp
  • A to G, chromosome 5 at 76,537,702 bp
  • A to G, chromosome 5 at 76,928,422 bp
  • A to C, chromosome 5 at 77,304,195 bp
  • A to C, chromosome 5 at 104,050,621 bp
  • A to G, chromosome 5 at 137,637,906 bp
  • C to A, chromosome 5 at 147,375,649 bp
  • A to C, chromosome 6 at 29,602,013 bp
  • T to C, chromosome 6 at 47,951,078 bp
  • T to A, chromosome 7 at 5,544,202 bp
  • G to A, chromosome 7 at 6,429,322 bp
  • A to T, chromosome 7 at 30,318,976 bp
  • T to C, chromosome 7 at 44,528,081 bp
  • T to A, chromosome 7 at 104,304,206 bp
  • T to A, chromosome 7 at 140,162,113 bp
  • C to A, chromosome 7 at 140,667,792 bp
  • C to G, chromosome 7 at 143,691,928 bp
  • G to A, chromosome 8 at 54,659,681 bp
  • A to G, chromosome 8 at 72,750,526 bp
  • T to A, chromosome 8 at 121,917,689 bp
  • A to T, chromosome 9 at 31,096,978 bp
  • A to T, chromosome 9 at 38,516,158 bp
  • A to G, chromosome 9 at 109,770,093 bp
  • G to A, chromosome 10 at 11,291,102 bp
  • G to A, chromosome 10 at 42,572,784 bp
  • A to G, chromosome 10 at 63,421,679 bp
  • A to G, chromosome 10 at 94,737,906 bp
  • T to C, chromosome 10 at 128,317,634 bp
  • T to A, chromosome 11 at 22,095,370 bp
  • T to G, chromosome 11 at 53,692,863 bp
  • T to A, chromosome 11 at 106,005,253 bp
  • T to C, chromosome 11 at 116,221,365 bp
  • A to G, chromosome 11 at 119,347,812 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • A to G, chromosome 12 at 85,013,961 bp
  • A to T, chromosome 13 at 11,690,363 bp
  • T to C, chromosome 13 at 61,004,025 bp
  • C to G, chromosome 13 at 100,781,775 bp
  • A to G, chromosome 14 at 57,445,659 bp
  • A to T, chromosome 16 at 32,754,079 bp
  • A to G, chromosome 16 at 73,973,785 bp
  • C to A, chromosome 17 at 36,285,579 bp
  • C to T, chromosome 18 at 13,021,997 bp
  • A to T, chromosome 18 at 14,570,441 bp
  • A to T, chromosome 19 at 56,813,181 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042908-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.