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Strain Name:
C57BL/6J-MtgxR5330Btlr/Mmmh
Stock Number:
042912-MU
Citation ID:
RRID:MMRRC_042912-MU
Other Names:
R5330 (G1), C57BL/6J-MtgxR5330Btlr
Major Collection:

Strain Information

Rgs11
Name: regulator of G-protein signaling 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 50782
HGNC: HGNC:9993
Homologene: 77719
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Snta1
Name: syntrophin, acidic 1
Synonyms: alpha1-syntrophin, Snt1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20648
Homologene: 2331
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 24,179,321 bp
  • G to A, chromosome 1 at 127,959,720 bp
  • A to T, chromosome 1 at 128,298,529 bp
  • G to A, chromosome 1 at 188,728,381 bp
  • T to C, chromosome 2 at 72,484,698 bp
  • C to A, chromosome 2 at 145,640,346 bp
  • C to A, chromosome 2 at 154,378,020 bp
  • A to G, chromosome 3 at 28,542,018 bp
  • T to A, chromosome 3 at 88,245,468 bp
  • T to G, chromosome 3 at 108,268,070 bp
  • G to T, chromosome 3 at 153,474,191 bp
  • A to T, chromosome 3 at 157,069,276 bp
  • A to G, chromosome 4 at 33,082,583 bp
  • T to C, chromosome 4 at 56,800,001 bp
  • G to A, chromosome 4 at 116,144,404 bp
  • A to T, chromosome 4 at 130,022,202 bp
  • A to G, chromosome 4 at 132,463,066 bp
  • A to C, chromosome 4 at 133,277,624 bp
  • A to T, chromosome 4 at 141,382,830 bp
  • G to T, chromosome 4 at 151,041,302 bp
  • G to T, chromosome 5 at 5,740,422 bp
  • A to G, chromosome 5 at 24,369,904 bp
  • A to T, chromosome 5 at 53,186,009 bp
  • C to A, chromosome 5 at 63,806,516 bp
  • T to C, chromosome 5 at 101,798,495 bp
  • A to G, chromosome 5 at 114,635,543 bp
  • T to C, chromosome 6 at 3,483,377 bp
  • G to A, chromosome 6 at 41,660,332 bp
  • T to G, chromosome 6 at 67,423,495 bp
  • T to A, chromosome 6 at 73,074,590 bp
  • T to C, chromosome 6 at 85,479,906 bp
  • A to G, chromosome 6 at 121,638,416 bp
  • T to A, chromosome 7 at 13,259,985 bp
  • T to C, chromosome 7 at 14,413,754 bp
  • G to A, chromosome 7 at 19,436,983 bp
  • C to T, chromosome 7 at 19,606,705 bp
  • T to A, chromosome 7 at 25,913,989 bp
  • T to C, chromosome 7 at 30,133,504 bp
  • T to A, chromosome 7 at 35,615,926 bp
  • G to A, chromosome 7 at 41,863,960 bp
  • T to C, chromosome 7 at 48,642,934 bp
  • T to C, chromosome 7 at 80,260,509 bp
  • T to C, chromosome 7 at 80,458,936 bp
  • A to T, chromosome 7 at 105,754,602 bp
  • T to C, chromosome 7 at 119,943,648 bp
  • T to A, chromosome 7 at 120,503,377 bp
  • T to C, chromosome 7 at 130,733,528 bp
  • T to C, chromosome 7 at 141,192,940 bp
  • A to T, chromosome 7 at 144,763,546 bp
  • T to A, chromosome 8 at 46,255,453 bp
  • A to T, chromosome 8 at 91,126,724 bp
  • T to G, chromosome 8 at 107,416,249 bp
  • A to G, chromosome 8 at 109,934,035 bp
  • A to C, chromosome 9 at 42,337,856 bp
  • T to A, chromosome 9 at 87,015,638 bp
  • T to C, chromosome 9 at 110,381,633 bp
  • A to T, chromosome 9 at 119,230,596 bp
  • T to C, chromosome 9 at 121,752,768 bp
  • A to G, chromosome 10 at 14,131,420 bp
  • A to T, chromosome 10 at 28,587,080 bp
  • G to A, chromosome 10 at 49,132,771 bp
  • G to T, chromosome 10 at 63,307,799 bp
  • A to G, chromosome 10 at 89,589,494 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • C to T, chromosome 11 at 50,146,313 bp
  • A to G, chromosome 11 at 61,070,186 bp
  • T to C, chromosome 11 at 61,491,239 bp
  • T to C, chromosome 11 at 69,747,275 bp
  • G to A, chromosome 11 at 75,515,580 bp
  • A to G, chromosome 11 at 97,378,026 bp
  • T to C, chromosome 11 at 102,197,354 bp
  • A to G, chromosome 12 at 59,095,812 bp
  • C to A, chromosome 12 at 113,490,580 bp
  • A to T, chromosome 13 at 24,025,946 bp
  • A to T, chromosome 13 at 59,700,403 bp
  • G to A, chromosome 14 at 26,773,830 bp
  • T to A, chromosome 14 at 84,533,046 bp
  • T to C, chromosome 14 at 99,140,646 bp
  • C to T, chromosome 15 at 30,332,115 bp
  • T to A, chromosome 15 at 82,196,208 bp
  • C to A, chromosome 15 at 101,624,664 bp
  • A to T, chromosome 17 at 26,202,973 bp
  • C to A, chromosome 17 at 26,275,733 bp
  • G to A, chromosome 17 at 50,509,848 bp
  • T to C, chromosome 17 at 50,699,132 bp
  • A to G, chromosome 17 at 74,423,195 bp
  • G to A, chromosome 18 at 36,954,702 bp
  • T to C, chromosome 18 at 46,960,430 bp
  • A to G, chromosome 19 at 6,490,081 bp
  • A to G, chromosome 19 at 7,879,455 bp
  • A to G, chromosome 19 at 11,091,858 bp
  • A to G, chromosome 19 at 25,411,329 bp
  • A to T, chromosome 19 at 44,078,955 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5330 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042912-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.