Strain Name:
C57BL/6J-MtgxR5332Btlr/Mmmh
Stock Number:
042914-MU
Citation ID:
RRID:MMRRC_042914-MU
Other Names:
R5332 (G1), C57BL/6J-MtgxR5332Btlr
Major Collection:

Gene Information

Pxn
Name: paxillin
Synonyms: Pax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19303
HGNC: HGNC:9718
Homologene: 37697
Rtn4
Name: reticulon 4
Synonyms: 1110020G17Rik, C130026I10Rik, NOGO, NgA, Nogo-B, Nogo-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68585
Homologene: 10743
Lin9
Name: lin-9 homolog (C. elegans)
Synonyms: 2700022J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72568
Homologene: 35252
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: 6230412P20Rik, Elys
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226747
Homologene: 9142
Crhbp
Name: corticotropin releasing hormone binding protein
Synonyms: CRH-BP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 12919
VEGA: 13
HGNC: HGNC:2356
Homologene: 1418
Clec16a
Name: C-type lectin domain family 16, member A
Synonyms: 4932416N17Rik, curt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74374
Homologene: 71019
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: NG36, G9a, D17Ertd710e, Bat8, KMT1C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110147
Homologene: 48460
Kcnj3
Name: potassium inwardly-rectifying channel, subfamily J, member 3
Synonyms: Kir3.1, GIRK1, Kcnf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16519
HGNC: HGNC:6264
Homologene: 1687
Mpdz
Name: multiple PDZ domain crumbs cell polarity complex component
Synonyms: MUPP1, B930003D11Rik, multiple PDZ domain protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17475
HGNC: HGNC:7208
Homologene: 2841
Map3k2
Name: mitogen-activated protein kinase kinase kinase 2
Synonyms: Mekk2, MEK kinase 2, 9630061B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26405
VEGA: 18
HGNC: HGNC:6854
Homologene: 74576
Spink5
Name: serine peptidase inhibitor, Kazal type 5
Synonyms: LEKT1, 2310065D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72432
Homologene: 4987
Cmya5
Name: cardiomyopathy associated 5
Synonyms: Myospryn, 2310076E16Rik, 2310076E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC381317, LOC269160, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Mybpc3
Name: myosin binding protein C, cardiac
Synonyms: cardiac C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17868
HGNC: HGNC:7551
Homologene: 215
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20192
Homologene: 68151
Abcc12
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 12
Synonyms: MRP9, 4930467B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244562
Homologene: 57211
Otud7a
Name: OTU domain containing 7A
Synonyms: Cezanne 2 protein, Otud7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170711
Homologene: 15642
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4932433F15Rik, 4933425F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71145
Homologene: 12556
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210274
Homologene: 105965
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: GluR1, GluR-A, Glr-1, Glur-1, Glur1, GluRA, Glr1, 2900051M01Rik, HIPA1, GluA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Mmp1b
Name: matrix metallopeptidase 1b (interstitial collagenase)
Synonyms: Mcol-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83996
HGNC: HGNC:7155
Homologene: 130791
Olfr1489
Name: olfactory receptor 1489
Synonyms: GA_x6K02T2RE5P-3965668-3966609, MOR202-19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258628
Homologene: 45021
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384534
Homologene: 129605
Vmn2r121
Name: vomeronasal 2, receptor 121
Synonyms: EG625699
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100038941
Homologene: 129606
Zfp974
Name: zinc finger protein 974
Synonyms: 1700049G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73430
Il12a
Name: interleukin 12a
Synonyms: IL-12p35, p35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16159
HGNC: HGNC:5969
Homologene: 681
Ace
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Synonyms: CD143
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11421
HGNC: HGNC:2707
Homologene: 37351
Coro2b
Name: coronin, actin binding protein, 2B
Synonyms: E130012P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235431
HGNC: HGNC:2256
Homologene: 21228
Fbxo40
Name: F-box protein 40
Synonyms: 9830003A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207215
Homologene: 9459
Gm8444
Name: predicted gene 8444
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 667073
VEGA: 15
Cyp4f14
Name: cytochrome P450, family 4, subfamily f, polypeptide 14
Synonyms: 1300014O15Rik, leukotriene B4 omega hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64385
VEGA: 17
Homologene: 81872
Bax
Name: BCL2-associated X protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12028
HGNC: HGNC:959
Homologene: 7242
Ptrh1
Name: peptidyl-tRNA hydrolase 1 homolog
Synonyms: 2210013M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329384
Homologene: 6135
Scgb2b19
Name: secretoglobin, family 2B, member 19
Synonyms: Gm5894, Abpbg19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545947
Homologene: 83171
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 179,795,962 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • A to G, chromosome 1 at 188,351,079 bp
  • T to C, chromosome 2 at 32,776,746 bp
  • A to G, chromosome 2 at 55,437,547 bp
  • C to T, chromosome 2 at 91,122,938 bp
  • A to G, chromosome 2 at 112,902,693 bp
  • T to C, chromosome 3 at 68,694,155 bp
  • A to C, chromosome 4 at 81,292,580 bp
  • A to G, chromosome 5 at 115,544,369 bp
  • T to A, chromosome 7 at 10,169,125 bp
  • A to C, chromosome 7 at 27,926,290 bp
  • T to C, chromosome 7 at 33,278,581 bp
  • T to C, chromosome 7 at 45,466,771 bp
  • T to A, chromosome 7 at 63,735,826 bp
  • T to A, chromosome 7 at 144,411,292 bp
  • G to T, chromosome 8 at 86,524,830 bp
  • T to C, chromosome 9 at 7,384,897 bp
  • T to C, chromosome 9 at 62,429,230 bp
  • T to A, chromosome 11 at 29,733,645 bp
  • T to A, chromosome 11 at 57,327,621 bp
  • T to A, chromosome 11 at 105,973,879 bp
  • T to A, chromosome 13 at 93,096,195 bp
  • G to T, chromosome 13 at 95,436,455 bp
  • CG to C, chromosome 14 at 65,759,662 bp
  • T to C, chromosome 15 at 81,843,701 bp
  • G to A, chromosome 16 at 10,731,679 bp
  • T to C, chromosome 16 at 36,968,854 bp
  • T to C, chromosome 17 at 32,906,091 bp
  • T to A, chromosome 17 at 34,905,710 bp
  • A to G, chromosome 18 at 32,207,456 bp
  • A to G, chromosome 18 at 43,992,917 bp
  • A to T, chromosome 19 at 13,633,365 bp
  • T to C, chromosome X at 124,133,575 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5332 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042914-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.