Strain Name:
C57BL/6J-MtgxR5333Btlr/Mmmh
Stock Number:
042915-MU
Citation ID:
RRID:MMRRC_042915-MU
Other Names:
R5333 (G1), C57BL/6J-MtgxR5333Btlr
Major Collection:

Gene Information

Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: 3110083O19Rik, NICE-4, 4932431F02Rik, A430103N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74383
Homologene: 136291
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Katnb1
Name: katanin p80 (WD40-containing) subunit B 1
Synonyms: KAT, 2410003J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74187
HGNC: HGNC:6217
Homologene: 4302
Cdkal1
Name: CDK5 regulatory subunit associated protein 1-like 1
Synonyms: 6620401C13Rik, 1190005B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68916
Homologene: 9830
Ogdh
Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms: alpha-ketoglutarate dehydrogenase, 2210412K19Rik, 2210403E04Rik, d1401
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18293
HGNC: HGNC:8124
Homologene: 55662
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72416
Homologene: 32695
Rcn1
Name: reticulocalbin 1
Synonyms: Rcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19672
HGNC: HGNC:9934
Homologene: 20637
Pcbp2
Name: poly(rC) binding protein 2
Synonyms: alphaCP-2, Hnrpx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18521
VEGA: 15
HGNC: HGNC:8648
Homologene: 74536
Psma6
Name: proteasome subunit alpha 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26443
HGNC: HGNC:9535
Homologene: 2085
Nup88
Name: nucleoporin 88
Synonyms: Nup84, Prei2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19069
HGNC: HGNC:8067
Homologene: 1901
Cdc37
Name: cell division cycle 37
Synonyms: p50Cdc37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12539
VEGA: 9
HGNC: HGNC:1735
Homologene: 38268
Cenph
Name: centromere protein H
Synonyms: ENP, CENP-H, 1700021I11Rik, 2610042E16Rik, 2810046K12Rik, 2410018A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26886
Homologene: 32519
Bpnt2
Name: 3'(2'), 5'-bisphosphate nucleotidase 2
Synonyms: 1110001C20Rik, Jaws, gPAPP, Impad1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242291
Homologene: 9852
Thsd1
Name: thrombospondin, type I, domain 1
Synonyms: 4833423O18Rik, Tmtsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56229
Homologene: 10264
Ckap4
Name: cytoskeleton-associated protein 4
Synonyms: CLIMP-63, P63, 5630400A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216197
VEGA: 10
Homologene: 4970
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 544678
Homologene: 129584
Stk31
Name: serine threonine kinase 31
Synonyms: C330007K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 77485
Homologene: 12677
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Mast3
Name: microtubule associated serine/threonine kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 546071
Homologene: 66191
Arhgef15
Name: Rho guanine nucleotide exchange factor (GEF) 15
Synonyms: D530030K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 442801
Homologene: 18345
Odad4
Name: outer dynein arm complex subunit 4
Synonyms: 4933404O19Rik, Ttc25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74407
Homologene: 12860
Gbe1
Name: glucan (1,4-alpha-), branching enzyme 1
Synonyms: 2310045H19Rik, 2810426P10Rik, D16Ertd536e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74185
HGNC: HGNC:4180
Homologene: 129
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: LOC381522, E230008N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241116
Homologene: 28093
Abcb5
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 5
Synonyms: 9230106F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 77706
HGNC: HGNC:46
Homologene: 83488
Vil1
Name: villin 1
Synonyms: Villin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22349
Homologene: 5169
Abca14
Name: ATP-binding cassette, sub-family A (ABC1), member 14
Synonyms: 1700110B15Rik, 4930539G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67928
Homologene: 86128
Mmp1b
Name: matrix metallopeptidase 1b (interstitial collagenase)
Synonyms: Mcol-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83996
HGNC: HGNC:7155
Homologene: 130791
Syt10
Name: synaptotagmin X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54526
Homologene: 10303
Plin4
Name: perilipin 4
Synonyms: S3-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57435
Homologene: 69311
Olfr1131
Name: olfactory receptor 1131
Synonyms: GA_x6K02T2Q125-49133664-49134593, MOR177-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258652
Homologene: 133019
Myo18b
Name: myosin XVIIIb
Synonyms: 4932408L24Rik, 4933411E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74376
Homologene: 53435
Cldn13
Name: claudin 13
Synonyms: 2500004B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57255
Triml1
Name: tripartite motif family-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244448
Homologene: 18619
Itgax
Name: integrin alpha X
Synonyms: CD11C (p150) alpha polypeptide, Cd11c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16411
HGNC: HGNC:6152
Homologene: 55493
Fdxr
Name: ferredoxin reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14149
HGNC: HGNC:3642
Homologene: 3033
Mpp4
Name: membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Synonyms: DLG6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227157
Homologene: 23296
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75087
Homologene: 18988
Panx2
Name: pannexin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406218
HGNC: HGNC:8600
Homologene: 14155
Papss1
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Synonyms: SK1, Asapk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23971
HGNC: HGNC:8603
Homologene: 81740
Potea
Name: POTE ankyrin domain family member A
Synonyms: 4930430A15Rik, A26c3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67575
Homologene: 69410
Nkain3
Name: Na+/K+ transporting ATPase interacting 3
Synonyms: E130310K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269513
Homologene: 18265
Vmn1r175
Name: vomeronasal 1 receptor 175
Synonyms: Gm6299
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 622222
Homologene: 128341
Bhmt2
Name: betaine-homocysteine methyltransferase 2
Synonyms: D13Ucla2, C81077
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 64918
HGNC: HGNC:1048
Homologene: 49496
Cd209c
Name: CD209c antigen
Synonyms: SIGNR2, mSIGNR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170776
HGNC: HGNC:1641
Homologene: 87186
Ddn
Name: dendrin
Synonyms: LOC328602
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13199
VEGA: 15
Homologene: 12816
Iqcf3
Name: IQ motif containing F3
Synonyms: 1700012F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68265
Homologene: 86789
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 57344
VEGA: 19
Homologene: 10754
Slc11a1
Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Synonyms: host resistance locus Bcg/Ity/Lsh, Bcg, Ity, Lsh, Nramp, ity, Nramp1, Ity1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18173
Homologene: 73884
Olfr1293-ps
Name: olfactory receptor 1293, pseudogene
Synonyms: GA_x6K02T2Q125-72578807-72579745, MOR245-24P, OTTMUSG00000015076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 623583
Pcdhgb1
Name: protocadherin gamma subfamily B, 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93699
HGNC: HGNC:8708
Homologene: 81867
Pcdha7
Name: protocadherin alpha 7
Synonyms: Cnr4, Crnr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12939
HGNC: HGNC:8673
Homologene: 117962
Scgb2b18
Name: secretoglobin, family 2B, member 18
Synonyms: Gm4692, Abpbg18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043856
Homologene: 83171
AC099760.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 59,157,441 bp
  • A to T, chromosome 1 at 71,624,180 bp
  • A to C, chromosome 1 at 74,384,145 bp
  • G to A, chromosome 1 at 74,432,390 bp
  • A to T, chromosome 1 at 74,903,175 bp
  • A to C, chromosome 2 at 69,525,228 bp
  • A to G, chromosome 2 at 87,629,114 bp
  • A to T, chromosome 2 at 105,389,126 bp
  • A to T, chromosome 2 at 111,194,337 bp
  • A to G, chromosome 2 at 111,527,703 bp
  • A to G, chromosome 3 at 90,025,084 bp
  • T to A, chromosome 3 at 131,643,044 bp
  • A to C, chromosome 4 at 4,767,963 bp
  • T to A, chromosome 4 at 20,484,889 bp
  • A to T, chromosome 4 at 45,890,935 bp
  • A to G, chromosome 4 at 155,436,740 bp
  • T to C, chromosome 5 at 112,791,176 bp
  • A to T, chromosome 5 at 134,915,015 bp
  • T to A, chromosome 6 at 49,469,152 bp
  • T to C, chromosome 7 at 23,808,579 bp
  • A to T, chromosome 7 at 33,173,275 bp
  • T to A, chromosome 7 at 120,289,546 bp
  • A to G, chromosome 7 at 128,142,283 bp
  • A to G, chromosome 8 at 3,944,976 bp
  • T to A, chromosome 8 at 9,770,762 bp
  • T to A, chromosome 8 at 22,259,340 bp
  • C to T, chromosome 8 at 43,130,290 bp
  • A to G, chromosome 8 at 70,783,501 bp
  • A to T, chromosome 8 at 95,095,606 bp
  • T to C, chromosome 9 at 7,384,897 bp
  • T to C, chromosome 9 at 21,143,161 bp
  • A to G, chromosome 9 at 106,553,661 bp
  • C to A, chromosome 10 at 84,527,610 bp
  • T to G, chromosome 11 at 6,352,126 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • A to T, chromosome 11 at 59,062,692 bp
  • A to G, chromosome 11 at 68,947,196 bp
  • A to C, chromosome 11 at 70,945,016 bp
  • A to G, chromosome 11 at 100,561,884 bp
  • T to C, chromosome 11 at 115,272,258 bp
  • A to G, chromosome 12 at 55,407,428 bp
  • A to T, chromosome 12 at 118,867,942 bp
  • T to C, chromosome 13 at 29,326,152 bp
  • A to G, chromosome 13 at 93,671,430 bp
  • G to T, chromosome 13 at 100,761,772 bp
  • T to G, chromosome 15 at 64,127,414 bp
  • T to C, chromosome 15 at 89,068,539 bp
  • T to A, chromosome 15 at 89,841,729 bp
  • T to C, chromosome 15 at 98,805,356 bp
  • T to G, chromosome 15 at 102,486,021 bp
  • A to T, chromosome 16 at 70,529,002 bp
  • T to G, chromosome 17 at 34,690,231 bp
  • A to G, chromosome 17 at 56,104,970 bp
  • C to A, chromosome 17 at 84,790,393 bp
  • A to T, chromosome 18 at 36,974,566 bp
  • C to T, chromosome 18 at 37,685,424 bp
  • A to G, chromosome 19 at 46,708,196 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5333 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042915-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.