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Strain Name:
C57BL/6J-MtgxR5335Btlr/Mmmh
Stock Number:
042916-MU
Citation ID:
RRID:MMRRC_042916-MU
Other Names:
R5335 (G1), C57BL/6J-MtgxR5335Btlr
Major Collection:

Strain Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Epc2
Name: enhancer of polycomb homolog 2
Synonyms: D2Ertd694e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227867
Homologene: 32274
Mcph1
Name: microcephaly, primary autosomal recessive 1
Synonyms: 5430437K10Rik, BRIT1, D030046N04Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244329
HGNC: HGNC:6954
Homologene: 32586
Phip
Name: pleckstrin homology domain interacting protein
Synonyms: Wdr11, Ndrp, 2810004D21Rik, 4632404O06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 83946
Homologene: 41209
Epha8
Name: Eph receptor A8
Synonyms: EphA8, Hek3, Eek
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13842
HGNC: HGNC:3391
Homologene: 22436
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,463,178 bp
  • T to A, chromosome 1 at 59,004,234 bp
  • C to G, chromosome 1 at 85,589,118 bp
  • A to T, chromosome 1 at 166,497,453 bp
  • T to C, chromosome 1 at 173,926,919 bp
  • A to T, chromosome 2 at 36,688,094 bp
  • A to G, chromosome 2 at 49,513,230 bp
  • T to G, chromosome 2 at 87,049,165 bp
  • T to C, chromosome 2 at 91,634,932 bp
  • A to T, chromosome 2 at 143,546,640 bp
  • G to T, chromosome 3 at 88,326,655 bp
  • A to G, chromosome 3 at 103,175,635 bp
  • G to A, chromosome 3 at 107,624,671 bp
  • A to G, chromosome 3 at 114,095,240 bp
  • T to C, chromosome 4 at 32,818,651 bp
  • T to C, chromosome 4 at 41,190,846 bp
  • C to A, chromosome 4 at 94,556,807 bp
  • T to C, chromosome 4 at 96,307,352 bp
  • G to A, chromosome 4 at 100,968,085 bp
  • T to C, chromosome 4 at 129,595,186 bp
  • A to T, chromosome 4 at 136,931,935 bp
  • A to G, chromosome 5 at 52,741,756 bp
  • A to T, chromosome 5 at 66,881,957 bp
  • A to T, chromosome 5 at 109,286,807 bp
  • T to A, chromosome 5 at 143,288,563 bp
  • T to C, chromosome 7 at 28,089,734 bp
  • A to C, chromosome 7 at 103,051,535 bp
  • A to C, chromosome 7 at 103,358,522 bp
  • A to C, chromosome 7 at 106,805,734 bp
  • A to G, chromosome 7 at 141,489,615 bp
  • A to T, chromosome 8 at 4,266,814 bp
  • T to A, chromosome 8 at 18,689,061 bp
  • A to G, chromosome 8 at 124,003,651 bp
  • A to G, chromosome 9 at 28,675,325 bp
  • T to A, chromosome 9 at 35,516,442 bp
  • T to C, chromosome 9 at 82,900,756 bp
  • A to T, chromosome 10 at 29,147,106 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • C to T, chromosome 10 at 121,785,377 bp
  • T to C, chromosome 11 at 5,717,754 bp
  • A to C, chromosome 11 at 51,027,334 bp
  • T to C, chromosome 11 at 118,112,514 bp
  • A to C, chromosome 13 at 21,638,779 bp
  • T to C, chromosome 13 at 22,328,191 bp
  • A to G, chromosome 14 at 26,879,738 bp
  • A to G, chromosome 14 at 54,986,563 bp
  • G to T, chromosome 14 at 74,742,113 bp
  • A to G, chromosome 14 at 99,097,653 bp
  • G to A, chromosome 15 at 55,045,740 bp
  • A to G, chromosome 15 at 85,336,584 bp
  • C to T, chromosome 16 at 32,298,584 bp
  • CGG to CGGG, chromosome 16 at 87,923,194 bp
  • T to C, chromosome 17 at 13,063,067 bp
  • T to C, chromosome 17 at 80,225,755 bp
  • C to T, chromosome 18 at 6,490,689 bp
  • A to T, chromosome 18 at 7,294,566 bp
  • G to T, chromosome 18 at 37,267,255 bp
  • A to G, chromosome 19 at 4,498,264 bp
  • A to T, chromosome 19 at 40,090,616 bp
  • C to A, chromosome 19 at 45,794,246 bp
  • C to T, chromosome 19 at 61,175,926 bp
  • A to T, chromosome Y at 2,663,647 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5335 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042916-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.