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Strain Name:
C57BL/6J-MtgxR5344Btlr/Mmmh
Stock Number:
042923-MU
Citation ID:
RRID:MMRRC_042923-MU
Other Names:
R5344 (G1), C57BL/6J-MtgxR5344Btlr
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Btc
Name: betacellulin, epidermal growth factor family member
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12223
HGNC: HGNC:1121
Homologene: 1309
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Epc1
Name: enhancer of polycomb homolog 1
Synonyms: A930032N02Rik, 5730566F07Rik, 2400007E14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13831
Homologene: 32627
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 166,379,512 bp
  • A to G, chromosome 1 at 173,131,106 bp
  • G to A, chromosome 1 at 178,322,033 bp
  • A to G, chromosome 2 at 63,979,225 bp
  • A to C, chromosome 2 at 88,310,990 bp
  • C to T, chromosome 2 at 91,645,243 bp
  • T to C, chromosome 2 at 122,281,871 bp
  • G to A, chromosome 2 at 132,558,677 bp
  • T to A, chromosome 3 at 52,103,725 bp
  • T to A, chromosome 3 at 114,208,362 bp
  • A to T, chromosome 3 at 145,087,942 bp
  • G to T, chromosome 4 at 8,844,417 bp
  • A to T, chromosome 4 at 14,519,402 bp
  • A to G, chromosome 4 at 20,021,245 bp
  • A to C, chromosome 4 at 41,251,578 bp
  • A to G, chromosome 4 at 72,852,115 bp
  • T to A, chromosome 4 at 145,178,334 bp
  • A to G, chromosome 4 at 148,153,573 bp
  • A to G, chromosome 5 at 14,676,612 bp
  • C to T, chromosome 5 at 73,104,774 bp
  • A to T, chromosome 5 at 91,376,920 bp
  • C to A, chromosome 5 at 120,822,204 bp
  • T to C, chromosome 5 at 121,343,676 bp
  • A to G, chromosome 6 at 37,057,443 bp
  • A to G, chromosome 6 at 38,158,228 bp
  • A to G, chromosome 6 at 78,372,860 bp
  • T to C, chromosome 6 at 85,696,789 bp
  • T to C, chromosome 6 at 91,266,383 bp
  • C to A, chromosome 6 at 118,465,179 bp
  • A to G, chromosome 6 at 146,649,185 bp
  • T to C, chromosome 7 at 85,875,838 bp
  • T to C, chromosome 7 at 141,276,524 bp
  • T to C, chromosome 7 at 142,968,699 bp
  • G to T, chromosome 8 at 4,185,990 bp
  • A to T, chromosome 8 at 25,043,047 bp
  • A to G, chromosome 8 at 80,985,763 bp
  • A to G, chromosome 8 at 84,958,106 bp
  • T to A, chromosome 8 at 93,337,193 bp
  • C to A, chromosome 8 at 114,422,972 bp
  • A to G, chromosome 9 at 85,735,004 bp
  • T to C, chromosome 9 at 119,534,007 bp
  • C to T, chromosome 10 at 13,253,616 bp
  • C to A, chromosome 10 at 75,246,173 bp
  • T to C, chromosome 10 at 88,570,568 bp
  • A to C, chromosome 11 at 31,101,114 bp
  • T to C, chromosome 11 at 62,820,925 bp
  • T to C, chromosome 11 at 74,084,495 bp
  • T to C, chromosome 11 at 80,971,587 bp
  • A to G, chromosome 11 at 106,399,811 bp
  • G to A, chromosome 11 at 115,989,749 bp
  • T to G, chromosome 12 at 55,255,581 bp
  • T to G, chromosome 12 at 71,243,027 bp
  • T to C, chromosome 12 at 80,953,766 bp
  • T to A, chromosome 12 at 104,035,548 bp
  • T to C, chromosome 12 at 113,131,566 bp
  • A to G, chromosome 13 at 92,521,588 bp
  • A to G, chromosome 13 at 115,002,309 bp
  • T to C, chromosome 14 at 15,358,591 bp
  • T to A, chromosome 14 at 46,767,350 bp
  • T to C, chromosome 14 at 56,794,062 bp
  • C to T, chromosome 15 at 27,735,532 bp
  • A to G, chromosome 15 at 82,371,638 bp
  • T to A, chromosome 16 at 17,257,848 bp
  • T to C, chromosome 16 at 44,416,400 bp
  • C to A, chromosome 17 at 38,209,104 bp
  • A to T, chromosome 17 at 66,049,848 bp
  • A to G, chromosome 17 at 78,926,113 bp
  • G to A, chromosome 18 at 6,450,614 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5344 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042923-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.