Strain Name:
C57BL/6J-MtgxR5348Btlr/Mmmh
Stock Number:
042927-MU
Citation ID:
RRID:MMRRC_042927-MU
Other Names:
R5348 (G1), C57BL/6J-MtgxR5348Btlr
Major Collection:

Gene Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 29869
Homologene: 5891
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Ddx55
Name: DEAD box helicase 55
Synonyms: 2810021H22Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67848
Homologene: 12196
Tdp1
Name: tyrosyl-DNA phosphodiesterase 1
Synonyms: 4921509N21Rik, SCAN1, 2810481F14Rik, E430034L06Rik, Gm40556
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104884
Homologene: 5424
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: Tweek, FSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Kat14
Name: lysine acetyltransferase 14
Synonyms: 2510008M08Rik, D2Ertd473e, D2Wsu131e, ATAC2, Csrp2bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228714
Homologene: 10745
Hmgxb4
Name: HMG box domain containing 4
Synonyms: 4733401K04Rik, Hmgb2l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70823
HGNC: HGNC:5003
Homologene: 4007
Abcg5
Name: ATP binding cassette subfamily G member 5
Synonyms: Sterolin-1, trac, cmp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27409
Homologene: 31909
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Chn2
Name: chimerin 2
Synonyms: 1700026N20Rik, 4930557O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Cux2
Name: cut-like homeobox 2
Synonyms: Cux2, Cux-2, ENSMUSG00000072641, Cutl2, 1700051K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13048
Homologene: 22426
Cdk12
Name: cyclin-dependent kinase 12
Synonyms: 1810022J16Rik, Crk7, D11Ertd752e, Crkrs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69131
Homologene: 128632
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Vip2, Hisppd1, Cfap160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227399
Homologene: 49409
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Olfml2b
Name: olfactomedin-like 2B
Synonyms: 1110018N05Rik, 4832415H08Rik, photomedin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320078
Homologene: 18546
Cyp4f18
Name: cytochrome P450, family 4, subfamily f, polypeptide 18
Synonyms: 1810054N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72054
HGNC: HGNC:2645
Homologene: 128623
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: DPD, E330028L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Lrrc7
Name: leucine rich repeat containing 7
Synonyms: densin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242274
Homologene: 10817
Tfip11
Name: tuftelin interacting protein 11
Synonyms: Tip39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54723
Homologene: 40813
Rapgef5
Name: Rap guanine nucleotide exchange factor (GEF) 5
Synonyms: mr-gef, D030051B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217944
VEGA: 12
Homologene: 56563
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Rnh1
Name: ribonuclease/angiogenin inhibitor 1
Synonyms: RNH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 107702
Homologene: 2204
Ppp1r9b
Name: protein phosphatase 1, regulatory subunit 9B
Synonyms: neurabin II, spinophilin, Spn, SPL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217124
HGNC: HGNC:9298
Homologene: 32787
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Kctd21
Name: potassium channel tetramerisation domain containing 21
Synonyms: EG622320
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 622320
Homologene: 54754
Lrfn2
Name: leucine rich repeat and fibronectin type III domain containing 2
Synonyms: 5730420O05Rik, SALM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70530
Homologene: 19182
Slco4c1
Name: solute carrier organic anion transporter family, member 4C1
Synonyms: SLC21A20, PRO2176, OATP4C1, OATP-M1, OATP-H, C330017E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227394
Homologene: 62654
Gpd1
Name: glycerol-3-phosphate dehydrogenase 1 (soluble)
Synonyms: Gdc-1, Gdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14555
HGNC: HGNC:4455
Homologene: 5593
Papolb
Name: poly (A) polymerase beta (testis specific)
Synonyms: TPAP, Papt, Plap-ps, Papola-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56522
Homologene: 121622
Olfr1084
Name: olfactory receptor 1084
Synonyms: GA_x6K02T2Q125-48121788-48120847, MOR192-2, MOR192-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258235
Homologene: 104285
Pramel12
Name: PRAME like 12
Synonyms: 4732496O08Rik, Pramef8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242736
Homologene: 128393
Gmfg
Name: glia maturation factor, gamma
Synonyms: 2310057N07Rik, 0610039G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 63986
HGNC: HGNC:4374
Homologene: 37978
Fbxo10
Name: F-box protein 10
Synonyms: FBX10, LOC269529
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269529
Homologene: 19544
Cep295nl
Name: CEP295 N-terminal like
Synonyms: Ddc8, BC100451
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 58251
Homologene: 75164
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 96,842,529 bp
  • A to G, chromosome 1 at 97,747,592 bp
  • A to G, chromosome 1 at 170,662,426 bp
  • T to C, chromosome 2 at 76,778,294 bp
  • A to T, chromosome 2 at 86,638,806 bp
  • G to A, chromosome 2 at 144,389,184 bp
  • A to G, chromosome 3 at 37,048,146 bp
  • T to A, chromosome 3 at 118,781,943 bp
  • T to A, chromosome 3 at 158,175,326 bp
  • A to G, chromosome 4 at 45,058,934 bp
  • T to C, chromosome 4 at 143,416,781 bp
  • C to T, chromosome 4 at 145,065,889 bp
  • A to G, chromosome 5 at 112,335,668 bp
  • A to G, chromosome 5 at 121,865,978 bp
  • T to A, chromosome 5 at 124,554,565 bp
  • T to C, chromosome 5 at 142,529,217 bp
  • A to G, chromosome 6 at 54,300,218 bp
  • G to A, chromosome 6 at 84,111,909 bp
  • A to G, chromosome 6 at 146,476,693 bp
  • A to G, chromosome 7 at 28,446,394 bp
  • A to G, chromosome 7 at 97,347,970 bp
  • A to T, chromosome 7 at 141,163,408 bp
  • A to G, chromosome 8 at 71,989,700 bp
  • T to A, chromosome 8 at 74,999,727 bp
  • T to C, chromosome 8 at 125,818,756 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • T to C, chromosome 11 at 61,783,613 bp
  • C to T, chromosome 11 at 79,564,899 bp
  • T to A, chromosome 11 at 94,996,612 bp
  • T to A, chromosome 11 at 98,204,292 bp
  • T to C, chromosome 11 at 118,333,599 bp
  • A to G, chromosome 12 at 99,915,506 bp
  • T to A, chromosome 12 at 117,688,611 bp
  • A to G, chromosome 13 at 55,312,334 bp
  • G to T, chromosome 14 at 14,786,310 bp
  • A to G, chromosome 14 at 52,232,698 bp
  • T to C, chromosome 15 at 99,722,140 bp
  • A to G, chromosome 17 at 49,096,690 bp
  • A to T, chromosome 17 at 84,671,206 bp
  • T to C, chromosome 18 at 31,983,919 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5348 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042927-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.