Strain Name:
C57BL/6J-MtgxR5349Btlr/Mmmh
Stock Number:
042928-MU
Citation ID:
RRID:MMRRC_042928-MU
Other Names:
R5349 (G1), C57BL/6J-MtgxR5349Btlr
Major Collection:

Strain Information

Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Fbl3a, Fbxl3a, Ovtm, Play68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50789
Homologene: 8127
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 208440
Homologene: 40996
Cnst
Name: consortin, connexin sorting protein
Synonyms: 9630058J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226744
Homologene: 17139
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: B3RP, Ae2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20535
Homologene: 128699
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93742
Homologene: 10489
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Diaph1
Name: diaphanous related formin 1
Synonyms: p140mDia, Drf1, Dia1, D18Wsu154e, mDia1, Diap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13367
HGNC: HGNC:2876
Homologene: 129567
Cul5
Name: cullin 5
Synonyms: 4921514I20Rik, C330021I08Rik, C030032G03Rik, VACM-1, 8430423K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75717
HGNC: HGNC:2556
Homologene: 2597
Ptprj
Name: protein tyrosine phosphatase, receptor type, J
Synonyms: Byp, DEP-1, Scc-1, Scc1, CD148, RPTPJ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19271
HGNC: HGNC:9673
Homologene: 2130
Glb1
Name: galactosidase, beta 1
Synonyms: Bgs, Bge, Bgt, Bgl, Bgl-t, Bgl-s, Bgl-e, C130097A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12091
VEGA: 9
HGNC: HGNC:4298
Homologene: 47922
Cd209d
Name: CD209d antigen
Synonyms: SIGNR3, mSIGNR3, SIGN-R3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170779
HGNC: HGNC:1641
Homologene: 8603
Myo15a
Name: myosin XVA
Synonyms: Myo15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 545030
Homologene: 83473
Pilra
Name: paired immunoglobin-like type 2 receptor alpha
Synonyms: FDF03
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231805
Homologene: 8387
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: NMDAR2B, NR2B, Nmdar2b, GluRepsilon2, GluN2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Stox2
Name: storkhead box 2
Synonyms: 4933409N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71069
Homologene: 18414
Zyg11a
Name: zyg-11 family member A, cell cycle regulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230590
Homologene: 66294
Tlr11
Name: toll-like receptor 11
Synonyms: LOC239081
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239081
Homologene: 77905
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Eif4a3l1
Name: eukaryotic translation initiation factor 4A3 like 1
Synonyms: Gm8994, B020013A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 668137
Pde7b
Name: phosphodiesterase 7B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 29863
HGNC: HGNC:8792
Homologene: 32194
Or4k2
Name: olfactory receptor family 4 subfamily K member 2
Synonyms: GA_x6K02T2PMLR-5881670-5880717, MOR247-1, Olfr730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258486
Homologene: 17269
Ogfod1
Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
Synonyms: 4930415J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270086
Homologene: 41238
Gm8220
Name: predicted gene 8220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 666660
Homologene: 128452
Srxn1
Name: sulfiredoxin 1 homolog (S. cerevisiae)
Synonyms: 1700127B04Rik, Srx, Npn3, Srx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76650
Homologene: 32722
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 179,622,897 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • A to C, chromosome 2 at 76,808,106 bp
  • A to G, chromosome 2 at 90,471,261 bp
  • G to A, chromosome 2 at 152,105,879 bp
  • T to C, chromosome 3 at 114,152,632 bp
  • A to T, chromosome 4 at 108,183,732 bp
  • A to G, chromosome 4 at 156,179,445 bp
  • T to C, chromosome 5 at 24,435,635 bp
  • T to A, chromosome 5 at 137,831,226 bp
  • A to G, chromosome 6 at 136,044,283 bp
  • A to T, chromosome 6 at 136,329,696 bp
  • T to G, chromosome 8 at 3,878,320 bp
  • A to G, chromosome 8 at 47,287,916 bp
  • T to G, chromosome 8 at 94,055,248 bp
  • G to T, chromosome 8 at 127,415,743 bp
  • T to A, chromosome 9 at 53,655,329 bp
  • T to C, chromosome 9 at 114,434,461 bp
  • C to T, chromosome 10 at 20,619,186 bp
  • T to C, chromosome 11 at 60,493,583 bp
  • C to A, chromosome 13 at 9,622,653 bp
  • T to C, chromosome 14 at 32,988,899 bp
  • T to C, chromosome 14 at 44,288,177 bp
  • G to T, chromosome 14 at 50,186,773 bp
  • T to C, chromosome 14 at 50,360,880 bp
  • T to C, chromosome 14 at 103,095,576 bp
  • A to G, chromosome 18 at 37,891,072 bp
  • T to C, chromosome 19 at 4,867,958 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5349 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042928-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.