Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5354Btlr/Mmmh
Stock Number:
042933-MU
Citation ID:
RRID:MMRRC_042933-MU
Other Names:
R5354 (G1), C57BL/6J-MtgxR5354Btlr
Major Collection:

Strain Information

Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Slc6a1
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: XT-1, Gabt, Xtrp1, Gat1, Gabt1, GAT-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232333
Homologene: 2290
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 6,844,171 bp
  • T to A, chromosome 1 at 22,538,511 bp
  • T to C, chromosome 1 at 131,180,648 bp
  • C to T, chromosome 1 at 150,445,924 bp
  • T to C, chromosome 1 at 162,830,145 bp
  • T to C, chromosome 1 at 174,430,686 bp
  • T to A, chromosome 2 at 91,059,353 bp
  • T to A, chromosome 2 at 127,428,723 bp
  • T to A, chromosome 2 at 170,349,396 bp
  • A to G, chromosome 2 at 182,000,809 bp
  • T to C, chromosome 3 at 29,082,212 bp
  • A to G, chromosome 3 at 89,221,377 bp
  • T to C, chromosome 3 at 89,379,606 bp
  • A to T, chromosome 3 at 89,997,955 bp
  • T to C, chromosome 3 at 98,712,315 bp
  • A to T, chromosome 3 at 144,737,005 bp
  • T to C, chromosome 4 at 40,180,290 bp
  • A to T, chromosome 4 at 63,080,028 bp
  • T to C, chromosome 4 at 112,711,593 bp
  • C to A, chromosome 4 at 115,433,464 bp
  • C to A, chromosome 5 at 8,090,182 bp
  • C to A, chromosome 5 at 14,678,808 bp
  • A to C, chromosome 5 at 41,831,537 bp
  • A to T, chromosome 5 at 113,846,165 bp
  • A to T, chromosome 5 at 114,936,996 bp
  • A to G, chromosome 5 at 137,380,788 bp
  • A to G, chromosome 5 at 139,766,428 bp
  • T to A, chromosome 6 at 34,814,752 bp
  • C to A, chromosome 6 at 58,215,596 bp
  • T to G, chromosome 6 at 83,183,126 bp
  • T to C, chromosome 6 at 114,302,623 bp
  • T to C, chromosome 6 at 119,968,523 bp
  • T to C, chromosome 6 at 124,859,637 bp
  • T to C, chromosome 7 at 28,362,274 bp
  • G to T, chromosome 7 at 29,195,873 bp
  • T to A, chromosome 7 at 48,198,329 bp
  • T to A, chromosome 7 at 79,725,721 bp
  • T to A, chromosome 7 at 84,916,149 bp
  • A to T, chromosome 8 at 12,806,753 bp
  • A to C, chromosome 8 at 22,177,565 bp
  • A to G, chromosome 8 at 105,425,364 bp
  • A to G, chromosome 8 at 109,555,746 bp
  • T to A, chromosome 9 at 53,589,183 bp
  • T to C, chromosome 9 at 58,157,612 bp
  • C to T, chromosome 9 at 86,835,124 bp
  • G to A, chromosome 10 at 4,534,188 bp
  • A to G, chromosome 10 at 20,333,532 bp
  • A to T, chromosome 10 at 61,348,661 bp
  • T to C, chromosome 10 at 79,898,396 bp
  • G to T, chromosome 10 at 80,323,689 bp
  • A to G, chromosome 11 at 29,154,166 bp
  • T to A, chromosome 11 at 29,211,646 bp
  • A to G, chromosome 11 at 35,675,913 bp
  • A to T, chromosome 11 at 67,255,725 bp
  • A to G, chromosome 11 at 77,686,277 bp
  • G to C, chromosome 13 at 58,610,778 bp
  • C to T, chromosome 13 at 96,654,896 bp
  • A to G, chromosome 14 at 20,464,909 bp
  • G to A, chromosome 14 at 21,677,023 bp
  • G to A, chromosome 14 at 26,774,342 bp
  • A to G, chromosome 14 at 63,818,904 bp
  • T to C, chromosome 15 at 76,353,130 bp
  • T to C, chromosome 15 at 81,408,007 bp
  • A to G, chromosome 16 at 14,618,671 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • A to G, chromosome 16 at 25,684,355 bp
  • T to C, chromosome 16 at 35,920,729 bp
  • A to G, chromosome 16 at 58,670,842 bp
  • T to C, chromosome 16 at 91,655,739 bp
  • C to A, chromosome 17 at 8,341,060 bp
  • C to A, chromosome 17 at 8,961,980 bp
  • G to A, chromosome 17 at 27,887,515 bp
  • A to T, chromosome 18 at 20,087,575 bp
  • G to T, chromosome 18 at 37,444,791 bp
  • A to G, chromosome 18 at 37,748,206 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • A to G, chromosome 18 at 60,602,231 bp
  • T to C, chromosome 19 at 57,130,923 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5354 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042933-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.