Strain Name:
C57BL/6J-MtgxR5375Btlr/Mmmh
Stock Number:
042951-MU
Citation ID:
RRID:MMRRC_042951-MU
Other Names:
R5375 (G1), C57BL/6J-MtgxR5375Btlr
Major Collection:

Strain Information

Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Mras
Name: muscle and microspikes RAS
Synonyms: 2900078C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17532
HGNC: HGNC:7227
Homologene: 7424
Mrpl39
Name: mitochondrial ribosomal protein L39
Synonyms: MRP-L5, Rpml5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27393
Homologene: 9679
Hcrtr1
Name: hypocretin (orexin) receptor 1
Synonyms: OX1R
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230777
HGNC: HGNC:4848
Homologene: 37492
Sec23a
Name: SEC23 homolog A, COPII coat complex component
Synonyms: Sec23r, Msec23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20334
Homologene: 4642
Smarcc1
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Synonyms: BAF155, SRG3, msp3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20588
Homologene: 68296
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Aldh4a1
Name: aldehyde dehydrogenase 4 family, member A1
Synonyms: Ahd-1, Ssdh1, ALDH4, A930035F14Rik, Ahd1, P5CDH
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 212647
HGNC: HGNC:406
Homologene: 6081
Zfp35
Name: zinc finger protein 35
Synonyms: Zfp-35
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 22694
Homologene: 133081
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67772
Homologene: 72405
Phf11d
Name: PHD finger protein 11D
Synonyms: D14Ertd668e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219132
Homologene: 87807
Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230249
Homologene: 6056
Calcr
Name: calcitonin receptor
Synonyms: Clr
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12311
HGNC: HGNC:1440
Homologene: 1320
Sun2
Name: Sad1 and UNC84 domain containing 2
Synonyms: B230369L08Rik, Unc84b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223697
Homologene: 9113
Fgfr2
Name: fibroblast growth factor receptor 2
Synonyms: Bek, KGFRTr, Fgfr-7, Fgfr-2, Fgfr7, svs, Fgfr2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14183
HGNC: HGNC:3689
Homologene: 22566
Rasa1
Name: RAS p21 protein activator 1
Synonyms: p120-rasGAP, Gap
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218397
HGNC: HGNC:9871
Homologene: 2168
Vps4b
Name: vacuolar protein sorting 4B
Synonyms: Skd1, 8030489C12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20479
Homologene: 37976
Bloc1s1
Name: biogenesis of lysosomal organelles complex-1, subunit 1
Synonyms: Gcn5l1, BLOS1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14533
HGNC: HGNC:4200
Homologene: 1140
Ccdc43
Name: coiled-coil domain containing 43
Synonyms: 2610001E01Rik, D11Ertd707e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52715
Homologene: 12074
Babam2
Name: BRISC and BRCA1 A complex member 2
Synonyms: 6030405P19Rik, B830038C02Rik, Bre
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 107976
HGNC: HGNC:1106
Homologene: 3604
Trp53inp1
Name: transformation related protein 53 inducible nuclear protein 1
Synonyms: 2700057G22Rik, SIP, Teap, SIP27, SIP18, Stinp, Tp53inp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 60599
Homologene: 11065
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Eif5b
Name: eukaryotic translation initiation factor 5B
Synonyms: IF2, A030003E17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226982
Homologene: 134613
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230866
Homologene: 9002
Dpp9
Name: dipeptidylpeptidase 9
Synonyms: DPRP2, 6430584G11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224897
VEGA: 17
Homologene: 16385
Invs
Name: inversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16348
Homologene: 7786
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 665700
Homologene: 90772
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 4831440I19Rik, 1500031A17Rik, C530008O15Rik, C630040K21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Zmiz2
Name: zinc finger, MIZ-type containing 2
Synonyms: 2410117E06Rik, D11Bwg0280e, Zimp7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52915
Homologene: 18000
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Cyp2b9
Name: cytochrome P450, family 2, subfamily b, polypeptide 9
Synonyms: Cyp2b, phenobarbitol inducible, type a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13094
HGNC: HGNC:2615
Homologene: 74933
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: PRIP, ASC2, RAP250, AIB3, NRC, ASC-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56406
Homologene: 137337
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: A530024P18Rik, 2310008C07Rik, 2310003D02Rik, mXin beta, myomaxin, Cmya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241431
Homologene: 19388
Adcy3
Name: adenylate cyclase 3
Synonyms: AC3, ACIII
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104111
HGNC: HGNC:234
Homologene: 2978
Elovl3
Name: ELOVL fatty acid elongase 3
Synonyms: CIN-2, Cig30, elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12686
Homologene: 69006
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: mSNM1B, Apollo, SNMIB
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 140917
Homologene: 32553
Ttll9
Name: tubulin tyrosine ligase-like family, member 9
Synonyms: 4930509O20Rik, 1700016F23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74711
Homologene: 41769
Or10a2
Name: olfactory receptor family 10 subfamily A member 2
Synonyms: GA_x6K02T2PBJ9-9453401-9454354, MOR263-2, P4, Olfr714
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259035
Homologene: 72055
Nlrc3
Name: NLR family, CARD domain containing 3
Synonyms: CLR16.2, D230007K08Rik, Caterpiller 16.2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 268857
Homologene: 18720
Fam234b
Name: family with sequence similarity 234, member B
Synonyms: 8430419L09Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74525
Homologene: 12572
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 435626
Homologene: 52359
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225638
Homologene: 50475
Cyld
Name: CYLD lysine 63 deubiquitinase
Synonyms: CYLD1, 2010013M14Rik, C130039D01Rik, 2900009M21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74256
HGNC: HGNC:2584
Homologene: 9069
Drc1
Name: dynein regulatory complex subunit 1
Synonyms: LOC381738, Gm1060, b2b1654Clo, Ccdc164
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381738
Homologene: 35977
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: Neu, Neu oncogene, c-erbB2, HER-2, ErbB-2, HER2, c-neu, l11Jus8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Sipa1
Name: signal-induced proliferation associated gene 1
Synonyms: SPA-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20469
VEGA: 19
Homologene: 7940
Or7g18
Name: olfactory receptor family 7 subfamily G member 18
Synonyms: GA_x6K02T2PVTD-12618399-12619337, MOR152-1, Olfr830
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258559
HGNC: HGNC:8466
Homologene: 79360
Cdh15
Name: cadherin 15
Synonyms: Cdh14, Mcad, M cadherin
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Lgr5
Name: leucine rich repeat containing G protein coupled receptor 5
Synonyms: Gpr49
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14160
HGNC: HGNC:4504
Homologene: 20807
Creb5
Name: cAMP responsive element binding protein 5
Synonyms: D430026C09Rik, Crebpa, 9430076C15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231991
Homologene: 18215
Zfpm1
Name: zinc finger protein, multitype 1
Synonyms: Friend of GATA-1, Fog1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22761
Homologene: 7606
Dtx3l
Name: deltex 3-like, E3 ubiquitin ligase
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209200
Homologene: 51375
Efhb
Name: EF hand domain family, member B
Synonyms: 4921525D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211482
VEGA: 17
Homologene: 16982
Snx31
Name: sorting nexin 31
Synonyms: 4631426E05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66696
Homologene: 23551
Or1j13
Name: olfactory receptor family 1 subfamily J member 13
Synonyms: GA_x6K02T2NLDC-33174915-33173974, MOR136-2, Olfr341
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258952
Homologene: 74160
Tmem74
Name: transmembrane protein 74
Synonyms: B230382K22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239408
VEGA: 15
Homologene: 17684
Etnk2
Name: ethanolamine kinase 2
Synonyms: 4933417N20Rik, Eki2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214253
Homologene: 10072
Or5k16
Name: olfactory receptor family 5 subfamily K member 16
Synonyms: GA_x54KRFPKG5P-55134972-55134019, MOR184-9, MOR184-11P, MOR184-11P, Olfr1563-ps1, Olfr180
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258178
Homologene: 79337
Efcab9
Name: EF-hand calcium binding domain 9
Synonyms: 4930403C08Rik, 1700007I06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69306
Homologene: 12309
Cst8
Name: cystatin 8 (cystatin-related epididymal spermatogenic)
Synonyms: Cst-rs1, Cres
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13012
HGNC: HGNC:2480
Homologene: 4011
Gm26657
Name: predicted gene, 26657
Type: Gene
Species: Mouse
Chromosome: 4
Gm12316
Name: predicted gene 12316
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 38,045,754 bp
  • A to G, chromosome 1 at 45,347,899 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 106,791,692 bp
  • A to T, chromosome 1 at 133,368,877 bp
  • T to C, chromosome 2 at 31,430,441 bp
  • A to G, chromosome 2 at 36,479,297 bp
  • T to C, chromosome 2 at 52,212,584 bp
  • A to T, chromosome 2 at 67,511,906 bp
  • A to G, chromosome 2 at 148,804,583 bp
  • G to T, chromosome 2 at 152,984,224 bp
  • A to T, chromosome 2 at 155,433,995 bp
  • A to T, chromosome 3 at 5,412,425 bp
  • T to C, chromosome 3 at 28,594,092 bp
  • C to T, chromosome 3 at 103,803,974 bp
  • A to G, chromosome 4 at 11,165,305 bp
  • G to A, chromosome 4 at 48,385,262 bp
  • A to G, chromosome 4 at 56,741,180 bp
  • T to A, chromosome 4 at 58,809,401 bp
  • C to T, chromosome 4 at 130,135,725 bp
  • T to A, chromosome 4 at 139,366,491 bp
  • T to C, chromosome 4 at 139,633,922 bp
  • A to T, chromosome 5 at 30,356,401 bp
  • T to A, chromosome 5 at 31,701,863 bp
  • G to T, chromosome 6 at 3,714,651 bp
  • A to T, chromosome 6 at 53,681,017 bp
  • A to T, chromosome 6 at 73,123,855 bp
  • A to T, chromosome 6 at 113,568,712 bp
  • C to A, chromosome 6 at 135,233,357 bp
  • A to C, chromosome 7 at 26,187,742 bp
  • CAGGGCCA to C, chromosome 7 at 80,098,340 bp
  • T to C, chromosome 7 at 80,513,229 bp
  • T to A, chromosome 7 at 107,073,873 bp
  • T to C, chromosome 7 at 130,241,215 bp
  • A to G, chromosome 8 at 88,733,036 bp
  • A to G, chromosome 8 at 122,336,073 bp
  • A to T, chromosome 8 at 122,865,100 bp
  • A to G, chromosome 9 at 18,876,146 bp
  • T to C, chromosome 9 at 66,467,887 bp
  • T to G, chromosome 9 at 99,394,616 bp
  • T to A, chromosome 9 at 110,190,949 bp
  • C to T, chromosome 10 at 115,478,564 bp
  • T to C, chromosome 10 at 128,923,957 bp
  • C to T, chromosome 11 at 6,397,519 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 32,527,484 bp
  • T to C, chromosome 11 at 55,262,820 bp
  • C to A, chromosome 11 at 70,611,290 bp
  • C to A, chromosome 11 at 98,433,412 bp
  • C to T, chromosome 11 at 102,690,232 bp
  • T to A, chromosome 12 at 4,210,870 bp
  • A to G, chromosome 12 at 59,007,005 bp
  • A to T, chromosome 13 at 85,288,903 bp
  • T to C, chromosome 14 at 52,204,154 bp
  • A to G, chromosome 14 at 57,638,307 bp
  • T to C, chromosome 14 at 59,352,671 bp
  • A to G, chromosome 15 at 36,525,584 bp
  • C to T, chromosome 15 at 43,867,168 bp
  • A to G, chromosome 15 at 79,727,522 bp
  • A to G, chromosome 16 at 3,964,753 bp
  • A to T, chromosome 16 at 35,933,027 bp
  • A to T, chromosome 16 at 37,082,784 bp
  • T to C, chromosome 16 at 58,915,885 bp
  • A to G, chromosome 16 at 84,723,902 bp
  • T to A, chromosome 17 at 53,401,626 bp
  • A to C, chromosome 17 at 56,189,424 bp
  • T to A, chromosome 18 at 24,002,916 bp
  • A to T, chromosome 18 at 65,372,738 bp
  • T to C, chromosome 18 at 82,597,688 bp
  • A to G, chromosome 19 at 5,659,612 bp
  • T to C, chromosome 19 at 46,134,696 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5375 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042951-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.