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Strain Name:
C57BL/6J-MtgxR5391Btlr/Mmmh
Stock Number:
042963-MU
Citation ID:
RRID:MMRRC_042963-MU
Other Names:
R5391 (G1), C57BL/6J-MtgxR5391Btlr
Major Collection:

Strain Information

Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik, D630049N15
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Nfia
Name: nuclear factor I/A
Synonyms: 1110047K16Rik, NF1-A, 9430022M17Rik, NF1A
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Tbce
Name: tubulin-specific chaperone E
Synonyms: 2610206D02Rik, C530005D02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70430
Homologene: 37744
Ctdspl2
Name: CTD small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329506
Homologene: 32311
Rangap1
Name: RAN GTPase activating protein 1
Synonyms: Fug1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19387
VEGA: 15
HGNC: HGNC:9854
Homologene: 55700
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 36,348,686 bp
  • A to T, chromosome 1 at 73,990,409 bp
  • A to G, chromosome 2 at 26,440,032 bp
  • T to A, chromosome 2 at 29,737,965 bp
  • A to G, chromosome 2 at 120,362,877 bp
  • G to A, chromosome 2 at 122,004,148 bp
  • G to A, chromosome 3 at 104,802,533 bp
  • A to G, chromosome 4 at 56,743,661 bp
  • A to G, chromosome 4 at 58,486,902 bp
  • A to G, chromosome 4 at 97,783,301 bp
  • T to C, chromosome 4 at 101,628,158 bp
  • A to T, chromosome 4 at 149,659,131 bp
  • G to A, chromosome 5 at 8,805,481 bp
  • T to A, chromosome 5 at 138,097,101 bp
  • T to C, chromosome 5 at 145,195,794 bp
  • A to T, chromosome 6 at 28,242,993 bp
  • T to C, chromosome 6 at 48,844,696 bp
  • T to C, chromosome 6 at 67,292,420 bp
  • T to C, chromosome 6 at 116,582,847 bp
  • G to A, chromosome 7 at 25,340,289 bp
  • T to C, chromosome 7 at 44,863,584 bp
  • A to T, chromosome 7 at 99,935,160 bp
  • C to T, chromosome 7 at 120,090,076 bp
  • A to T, chromosome 8 at 47,863,871 bp
  • A to G, chromosome 8 at 86,017,468 bp
  • G to A, chromosome 8 at 93,976,048 bp
  • G to A, chromosome 8 at 122,633,395 bp
  • A to G, chromosome 9 at 31,053,305 bp
  • G to A, chromosome 9 at 57,105,673 bp
  • A to G, chromosome 9 at 74,849,180 bp
  • A to T, chromosome 9 at 88,917,615 bp
  • G to T, chromosome 9 at 113,691,518 bp
  • A to G, chromosome 10 at 62,456,747 bp
  • G to A, chromosome 10 at 78,752,854 bp
  • A to G, chromosome 10 at 79,356,657 bp
  • T to C, chromosome 11 at 53,433,797 bp
  • A to G, chromosome 11 at 64,994,294 bp
  • T to A, chromosome 11 at 115,985,068 bp
  • T to A, chromosome 12 at 72,941,701 bp
  • A to C, chromosome 12 at 119,170,741 bp
  • T to C, chromosome 13 at 14,005,965 bp
  • G to T, chromosome 13 at 62,454,818 bp
  • T to C, chromosome 13 at 74,480,546 bp
  • T to A, chromosome 14 at 21,624,816 bp
  • T to C, chromosome 15 at 31,325,206 bp
  • G to T, chromosome 15 at 64,094,052 bp
  • A to G, chromosome 15 at 81,706,446 bp
  • C to A, chromosome 15 at 101,951,828 bp
  • T to A, chromosome 16 at 44,217,463 bp
  • T to C, chromosome 17 at 80,275,081 bp
  • T to G, chromosome 18 at 37,742,587 bp
  • A to G, chromosome 19 at 3,349,260 bp
  • C to G, chromosome 19 at 4,833,482 bp
  • T to G, chromosome 19 at 6,071,033 bp
  • G to T, chromosome 19 at 13,027,786 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5391 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042963-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.