Strain Name:
C57BL/6J-MtgxR5404Btlr/Mmmh
Stock Number:
042975-MU
Citation ID:
RRID:MMRRC_042975-MU
Other Names:
R5404 (G1), C57BL/6J-MtgxR5404Btlr
Major Collection:

Gene Information

Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Rbm5
Name: RNA binding motif protein 5
Synonyms: D030069N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83486
HGNC: HGNC:9902
Homologene: 21177
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: 9030416H16Rik, E230024D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71521
Homologene: 22877
Cry1
Name: cryptochrome 1 (photolyase-like)
Synonyms: Phll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12952
VEGA: 10
HGNC: HGNC:2384
Homologene: 7042
Smg1
Name: SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Synonyms: 5430435M13Rik, 2610207I05Rik, C130002K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233789
Homologene: 56697
Depdc1b
Name: DEP domain containing 1B
Synonyms: XTP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218581
Homologene: 10157
Rps6ka5
Name: ribosomal protein S6 kinase, polypeptide 5
Synonyms: 3110005L17Rik, 6330404E13Rik, MSK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73086
VEGA: 12
Homologene: 48302
Gabpa
Name: GA repeat binding protein, alpha
Synonyms: GABPalpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14390
HGNC: HGNC:4071
Homologene: 1543
Ccnk
Name: cyclin K
Synonyms: CycK, CPR4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12454
VEGA: 12
HGNC: HGNC:1596
Homologene: 14748
Nedd1
Name: neural precursor cell expressed, developmentally down-regulated gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17997
VEGA: 10
HGNC: HGNC:7723
Homologene: 7439
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: l7Rn3, Ten-m4, ELM2, Doc4, Odz4, l(7)-3Rn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23966
Homologene: 8034
Npc1
Name: NPC intracellular cholesterol transporter 1
Synonyms: D18Ertd723e, nmf164, lcsd, A430089E03Rik, C85354, D18Ertd139e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18145
HGNC: HGNC:7897
Homologene: 228
Il17re
Name: interleukin 17 receptor E
Synonyms: Il25r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 57890
Homologene: 17109
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Lemd3
Name: LEM domain containing 3
Synonyms: Man1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 380664
Homologene: 8633
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Fance
Name: Fanconi anemia, complementation group E
Synonyms: 2810451D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72775
HGNC: HGNC:3586
Homologene: 11066
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: nmf252, bob, sals, mdfw, ahl, nmf181, USH1D, 4930542A03Rik, nmf112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22295
Homologene: 11142
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Clec4b2
Name: C-type lectin domain family 4, member b2
Synonyms: mDCAR1, F830043G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381809
Homologene: 128249
A1bg
Name: alpha-1-B glycoprotein
Synonyms: C44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 117586
VEGA: 15
HGNC: HGNC:5
Homologene: 11167
Dock10
Name: dedicator of cytokinesis 10
Synonyms: ZIZ3, Zizimin3, Jr4, A630054M16Rik, Jr5, 9330153B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210293
Homologene: 45952
Slc15a3
Name: solute carrier family 15, member 3
Synonyms: Ci1, cI-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 65221
Homologene: 69222
Nup210
Name: nucleoporin 210
Synonyms: gp210, Pom210, gp190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54563
Homologene: 41286
Psmc4
Name: proteasome (prosome, macropain) 26S subunit, ATPase, 4
Synonyms: CAR interacting protein 21, MIP224, CIP21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23996
HGNC: HGNC:9551
Homologene: 4744
Spem1
Name: sperm maturation 1
Synonyms: 1700095G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74288
Homologene: 45717
Narf
Name: nuclear prelamin A recognition factor
Synonyms: 4430402O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67608
Homologene: 57048
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: GluRdelta2, tpr, B230104L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Nfil3
Name: nuclear factor, interleukin 3, regulated
Synonyms: E4BP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18030
HGNC: HGNC:7787
Homologene: 3928
Tchh
Name: trichohyalin
Synonyms: Thh, AHF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99681
Homologene: 136273
Slc40a1
Name: solute carrier family 40 (iron-regulated transporter), member 1
Synonyms: Pcm, Dusg, FPN1, IREG1, MTP1, Ol5, ferroportin1, metal transporting protein 1, Slc11a3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 53945
Homologene: 40959
Olfr138
Name: olfactory receptor 138
Synonyms: GA_x6K02T2PSCP-2718585-2719523, MOR256-40P, Olfr89
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 170648
Homologene: 119758
Cpox
Name: coproporphyrinogen oxidase
Synonyms: nct, clone 560, cac, Cpo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12892
VEGA: 16
HGNC: HGNC:2321
Homologene: 76
Olfr559
Name: olfactory receptor 559
Synonyms: GA_x6K02T2PBJ9-5441154-5440198, MOR11-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259116
Homologene: 74124
Fermt1
Name: fermitin family member 1
Synonyms: 5830467P10Rik, Kindlin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241639
Homologene: 9773
Gpr162
Name: G protein-coupled receptor 162
Synonyms: A-2, Grca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14788
Homologene: 8400
Tuba3a
Name: tubulin, alpha 3A
Synonyms: Tuba3, M[a]3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22144
Homologene: 128229
Olfr1181
Name: olfactory receptor 1181
Synonyms: MOR225-9P, GA_x6K02T2Q125-49911636-49910701
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258060
Homologene: 86666
Gm26883
Name: predicted gene, 26883
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Gm19445
Name: predicted gene, 19445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 45,912,328 bp
  • A to G, chromosome 1 at 80,503,913 bp
  • C to T, chromosome 2 at 88,423,801 bp
  • T to C, chromosome 2 at 132,934,869 bp
  • A to T, chromosome 2 at 169,884,320 bp
  • A to C, chromosome 3 at 19,989,128 bp
  • A to G, chromosome 3 at 33,075,875 bp
  • A to G, chromosome 3 at 93,447,675 bp
  • C to A, chromosome 5 at 65,696,910 bp
  • T to A, chromosome 6 at 63,930,910 bp
  • A to T, chromosome 6 at 91,025,369 bp
  • T to C, chromosome 6 at 113,469,102 bp
  • A to T, chromosome 6 at 123,181,349 bp
  • A to G, chromosome 6 at 124,861,643 bp
  • G to T, chromosome 6 at 125,281,592 bp
  • A to G, chromosome 7 at 28,042,412 bp
  • A to T, chromosome 7 at 96,894,680 bp
  • T to A, chromosome 7 at 102,723,600 bp
  • T to C, chromosome 7 at 118,206,908 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • T to C, chromosome 9 at 107,765,553 bp
  • G to T, chromosome 10 at 60,304,691 bp
  • T to C, chromosome 10 at 85,184,419 bp
  • C to T, chromosome 10 at 92,716,192 bp
  • G to A, chromosome 10 at 120,931,958 bp
  • T to A, chromosome 11 at 69,820,937 bp
  • T to A, chromosome 11 at 121,242,626 bp
  • T to C, chromosome 12 at 100,616,093 bp
  • A to T, chromosome 12 at 108,195,623 bp
  • G to A, chromosome 13 at 52,968,055 bp
  • T to C, chromosome 13 at 108,357,463 bp
  • T to C, chromosome 15 at 60,919,696 bp
  • A to G, chromosome 16 at 49,159,978 bp
  • G to A, chromosome 16 at 58,675,286 bp
  • T to A, chromosome 16 at 84,860,463 bp
  • A to T, chromosome 17 at 28,318,060 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • T to A, chromosome 17 at 38,275,626 bp
  • A to G, chromosome 18 at 12,213,299 bp
  • A to G, chromosome 18 at 70,509,432 bp
  • T to C, chromosome 19 at 10,857,658 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5404 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042975-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.