Strain Name:
C57BL/6J-MtgxR5409Btlr/Mmmh
Stock Number:
042978-MU
Citation ID:
RRID:MMRRC_042978-MU
Other Names:
R5409 (G1), C57BL/6J-MtgxR5409Btlr
Major Collection:

Strain Information

Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66865
Homologene: 6078
Aurka
Name: aurora kinase A
Synonyms: Ayk1, Ark1, IAK, AIRK1, aurora A, Aurora-A, Stk6, IAK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20878
Homologene: 2670
Cenpm
Name: centromere protein M
Synonyms: 2610019I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66570
VEGA: 15
Homologene: 11438
Diaph1
Name: diaphanous related formin 1
Synonyms: p140mDia, Drf1, Dia1, D18Wsu154e, mDia1, Diap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13367
HGNC: HGNC:2876
Homologene: 129567
Larp4
Name: La ribonucleoprotein 4
Synonyms: D330037H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 207214
VEGA: 15
Homologene: 19173
Rprd1b
Name: regulation of nuclear pre-mRNA domain containing 1B
Synonyms: 2810446G03Rik, 2610304G08Rik, Crept
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70470
Homologene: 41426
Tanc2
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
Synonyms: 5730590C14Rik, 3526402J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77097
Homologene: 64680
Ufl1
Name: UFM1 specific ligase 1
Synonyms: Rcad, Maxer, 1810074P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67490
Homologene: 9093
Dis3
Name: DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
Synonyms: 2810028N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72662
VEGA: 14
Homologene: 6910
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: Lec2, lectomedin-2, 2900070I05Rik, Lphn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330814
Homologene: 8951
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Nid2
Name: nidogen 2
Synonyms: entactin-2, entactin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18074
VEGA: 14
Homologene: 40575
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1, G1-415-19, ferf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Anapc4
Name: anaphase promoting complex subunit 4
Synonyms: 2610306D21Rik, APC4, D5Ertd249e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52206
Homologene: 40873
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Plekhh2
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213556
VEGA: 17
Homologene: 35317
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Posh, 2200003J05Rik, Sh3md2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 59009
Homologene: 10988
Unc13c
Name: unc-13 homolog C
Synonyms: Munc13-3, Unc13h3, D9Ertd414e, 1500037O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208898
Homologene: 45443
Fcrl6
Name: Fc receptor-like 6
Synonyms: FcRH6, ENSMUSG00000070504, moFcRH6, mIFGP6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 677296
Homologene: 88414
Pomgnt2
Name: protein O-linked mannose beta 1,4-N-acetylglucosaminyltransferase 2
Synonyms: C85492, Gtdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215494
VEGA: 9
Homologene: 32795
Rp1l1
Name: retinitis pigmentosa 1 homolog like 1
Synonyms: Dcdc4, Rp1hl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 271209
VEGA: 14
Homologene: 105870
Asic1
Name: acid-sensing (proton-gated) ion channel 1
Synonyms: BNaC2, ASIC, ASIC1a, ASICalpha, ASIC1 beta, B530003N02Rik, ASIC1b, Accn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11419
VEGA: 15
HGNC: HGNC:100
Homologene: 121755
Gm12239
Name: predicted gene 12239
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 115485826
Col5a1
Name: collagen, type V, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12831
HGNC: HGNC:2209
Homologene: 55434
Kcnk4
Name: potassium channel, subfamily K, member 4
Synonyms: TRAAKt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16528
VEGA: 19
HGNC: HGNC:6279
Homologene: 7391
Or5h22
Name: olfactory receptor family 5 subfamily H member 22
Synonyms: GA_x54KRFPKG5P-55303207-55302284, MOR183-4, Olfr190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258392
Homologene: 131129
Acot11
Name: acyl-CoA thioesterase 11
Synonyms: BFIT1, 2010309H15Rik, Them1, 1110020M10Rik, Thea
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329910
Homologene: 11977
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Ccn5
Name: cellular communication network factor 5
Synonyms: rCop1, Crgr4, CCN5, Wisp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22403
Homologene: 2882
Pgbd5
Name: piggyBac transposable element derived 5
Synonyms: 2900019M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209966
Homologene: 11583
Rnf7l
Name: ring finger protein 7 like
Synonyms: Gm7075
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 631906
VEGA: 10
Abca7
Name: ATP-binding cassette, sub-family A (ABC1), member 7
Synonyms: Abc51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 27403
HGNC: HGNC:37
Homologene: 22783
Gm8258
Name: predicted gene 8258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 666726
Or5aq1b
Name: olfactory receptor family 5 subfamily AQ member 1B
Synonyms: GA_x6K02T2Q125-48565383-48564445, MOR172-2, Olfr1107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258841
Homologene: 131138
Gm4775
Name: predicted gene 4775
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 212084
VEGA: 14
Vmn1r65
Name: vomeronasal 1 receptor 65
Synonyms: V3R6, V1rd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81013
Homologene: 110799
Sike1
Name: suppressor of IKBKE 1
Synonyms: 2810005O12Rik, 5730470L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66641
Homologene: 32622
Or5ac16
Name: olfactory receptor family 5 subfamily AC member 16
Synonyms: GA_x54KRFPKG5P-55416332-55415412, MOR182-8, Olfr198
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258036
Homologene: 51800
Vmn2r30
Name: vomeronasal 2, receptor 30
Synonyms: V2r15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22306
Homologene: 113703
Spag8
Name: sperm associated antigen 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433700
Homologene: 8250
Smpd5
Name: sphingomyelin phosphodiesterase 5
Synonyms: Gm10345
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100503915
Homologene: 132205
9130221H12Rik
Name: RIKEN cDNA 9130221H12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77124
Igkv4-61
Name: immunoglobulin kappa chain variable 4-61
Synonyms: Gm16895
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546244
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 172,598,159 bp
  • T to C, chromosome 2 at 26,392,008 bp
  • C to T, chromosome 2 at 27,960,445 bp
  • T to C, chromosome 2 at 76,870,549 bp
  • T to C, chromosome 2 at 87,071,870 bp
  • T to A, chromosome 2 at 158,075,067 bp
  • G to A, chromosome 2 at 163,825,238 bp
  • T to G, chromosome 2 at 172,367,116 bp
  • C to T, chromosome 3 at 102,998,558 bp
  • T to A, chromosome 3 at 144,916,691 bp
  • A to G, chromosome 4 at 25,280,706 bp
  • T to A, chromosome 4 at 43,653,134 bp
  • A to C, chromosome 4 at 63,966,536 bp
  • A to T, chromosome 4 at 64,007,417 bp
  • C to T, chromosome 4 at 106,760,130 bp
  • A to G, chromosome 5 at 52,848,599 bp
  • T to A, chromosome 5 at 104,776,461 bp
  • G to A, chromosome 5 at 134,522,791 bp
  • T to C, chromosome 6 at 69,417,127 bp
  • T to C, chromosome 7 at 6,009,013 bp
  • A to G, chromosome 7 at 7,312,548 bp
  • A to G, chromosome 7 at 25,229,579 bp
  • G to A, chromosome 8 at 61,374,245 bp
  • A to G, chromosome 8 at 83,929,742 bp
  • T to A, chromosome 8 at 124,371,880 bp
  • T to A, chromosome 9 at 73,578,390 bp
  • A to T, chromosome 9 at 121,982,237 bp
  • A to T, chromosome 10 at 63,421,624 bp
  • T to C, chromosome 10 at 80,014,320 bp
  • A to T, chromosome 11 at 56,068,949 bp
  • T to C, chromosome 11 at 105,867,485 bp
  • T to C, chromosome 14 at 19,805,962 bp
  • A to G, chromosome 14 at 30,595,972 bp
  • C to T, chromosome 14 at 31,263,255 bp
  • T to C, chromosome 14 at 64,030,621 bp
  • A to T, chromosome 14 at 99,085,932 bp
  • A to T, chromosome 14 at 106,100,952 bp
  • C to A, chromosome 15 at 76,295,714 bp
  • T to C, chromosome 15 at 82,234,363 bp
  • GCACC to GCACCACC, chromosome 15 at 99,698,803 bp
  • T to C, chromosome 15 at 99,986,064 bp
  • C to T, chromosome 16 at 59,074,196 bp
  • A to G, chromosome 16 at 59,201,557 bp
  • T to C, chromosome 17 at 84,586,478 bp
  • T to C, chromosome 18 at 37,897,548 bp
  • A to G, chromosome 19 at 6,926,210 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5409 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042978-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.