Strain Name:
C57BL/6J-MtgxR5413Btlr/Mmmh
Stock Number:
042982-MU
Citation ID:
RRID:MMRRC_042982-MU
Other Names:
R5413 (G1), C57BL/6J-MtgxR5413Btlr
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Wwp2
Name: WW domain containing E3 ubiquitin protein ligase 2
Synonyms: 1300010O06Rik, AIP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66894
Homologene: 48490
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76089
Homologene: 35477
Tyw1
Name: tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Synonyms: Rsafd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100929
Homologene: 7068
Zwilch
Name: zwilch kinetochore protein
Synonyms: 2310031L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68014
Homologene: 32381
Vdac1
Name: voltage-dependent anion channel 1
Synonyms: Vdac5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22333
Homologene: 107244
Uvssa
Name: UV stimulated scaffold protein A
Synonyms: D330017J19Rik, 4933407H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71101
Homologene: 13807
Piwil1
Name: piwi-like RNA-mediated gene silencing 1
Synonyms: MIWI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57749
HGNC: HGNC:9007
Homologene: 37963
Clint1
Name: clathrin interactor 1
Synonyms: Epn4, C530049I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216705
Homologene: 133740
Wnt3a
Name: wingless-type MMTV integration site family, member 3A
Synonyms: Wnt-3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22416
Homologene: 22528
Homer1
Name: homer scaffolding protein 1
Synonyms: Ves-1, PSD-Zip45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26556
Homologene: 3155
Angptl3
Name: angiopoietin-like 3
Synonyms: hypl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30924
HGNC: HGNC:491
Homologene: 8499
Osbp
Name: oxysterol binding protein
Synonyms: 1110018F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76303
VEGA: 19
HGNC: HGNC:8503
Homologene: 97668
Paf1
Name: Paf1, RNA polymerase II complex component
Synonyms: 5730511K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54624
Homologene: 5441
Dennd2a
Name: DENN/MADD domain containing 2A
Synonyms: B930096L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 209773
Homologene: 35238
Dpy19l4
Name: dpy-19-like 4 (C. elegans)
Synonyms: LOC381510, Narg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381510
Homologene: 18773
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: 9430028L06Rik, 9030411M15Rik, Fam38b2, Piezo2, Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 667742
Homologene: 49695
Unc13b
Name: unc-13 homolog B
Synonyms: Unc13h2, Munc13-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22249
Homologene: 31376
Pcsk2
Name: proprotein convertase subtilisin/kexin type 2
Synonyms: prohormone convertase 2, Phpp-2, Nec-2, Nec2, PC2, SPC2, 6330411F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18549
HGNC: HGNC:8744
Homologene: 37640
Dcdc5
Name: doublecortin domain containing 5
Synonyms: 4732421G10Rik, EG436559
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329482
Homologene: 79722
Ttc39a
Name: tetratricopeptide repeat domain 39A
Synonyms: 4922503N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230603
Homologene: 17739
Trpm5
Name: transient receptor potential cation channel, subfamily M, member 5
Synonyms: Mtr1, Ltrpc5, 9430099A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56843
Homologene: 22818
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Gria1
Name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Synonyms: GluR1, GluR-A, Glr-1, Glur-1, Glur1, GluRA, Glr1, 2900051M01Rik, HIPA1, GluA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14799
HGNC: HGNC:4571
Homologene: 20226
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666060
Homologene: 8939
Iqsec1
Name: IQ motif and Sec7 domain 1
Synonyms: cI-43, D6Ertd349e, BRAG2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232227
Homologene: 82429
Or2n1d
Name: olfactory receptor family 2 subfamily N member 1D
Synonyms: MOR256-7, GA_x6K02T2PSCP-2779375-2780313, Olfr136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258803
Homologene: 119758
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231591
Homologene: 129606
Fars2
Name: phenylalanine-tRNA synthetase 2 (mitochondrial)
Synonyms: 2810431B21Rik, 6720478K01Rik, Fars1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69955
VEGA: 13
Homologene: 4788
Or6b13
Name: olfactory receptor family 6 subfamily B member 13
Synonyms: GA_x6K02T2PBJ9-42354580-42353624, MOR103-14P, Olfr524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258055
Homologene: 79347
Ldha
Name: lactate dehydrogenase A
Synonyms: lactate dehydrogenase-A, LDH-A, Ldh-1, l7R2, Ldh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16828
HGNC: HGNC:6535
Homologene: 56495
Or2y1b
Name: olfactory receptor family 2 subfamily Y member 1B
Synonyms: GA_x6K02T2QP88-6117098-6116163, MOR256-55, L45, Olfr10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18307
Homologene: 81347
Or14c44
Name: olfactory receptor family 14 subfamily C member 44
Synonyms: GA_x6K02T2NHDJ-9693313-9692378, MOR221-1P, MOR211-8P, MOR221-1P, MOR221-4, Olfr1531-ps1, Olfr301
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257958
Homologene: 128067
Tmem59
Name: transmembrane protein 59
Synonyms: thymic dendritic cell-derived factor 1, MTDCF1, 3110046P06Rik, 1110001M20Rik, D4Ertd20e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56374
HGNC: HGNC:1239
Homologene: 3583
Clk2
Name: CDC-like kinase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12748
HGNC: HGNC:2069
Homologene: 33303
Usp17lc
Name: ubiquitin specific peptidase 17-like C
Synonyms: Dub2b, Dub2, Dub-2, Usp17l5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13532
Homologene: 131315
Igdcc3
Name: immunoglobulin superfamily, DCC subclass, member 3
Synonyms: 2810401C09Rik, WI-14920, Punc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19289
VEGA: 9
HGNC: HGNC:9700
Homologene: 3590
Fbxl16
Name: F-box and leucine-rich repeat protein 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214931
VEGA: 17
Homologene: 17732
Hoxd3
Name: homeobox D3
Synonyms: Hox-5.5, Hox-4.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15434
HGNC: HGNC:5137
Homologene: 5034
9930111J21Rik2
Name: RIKEN cDNA 9930111J21 gene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245240
Esp24
Name: exocrine gland secreted peptide 24
Synonyms: Gm21947
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100126776
VEGA: 17
Igkv12-98
Name: immunoglobulin kappa variable 12-98
Synonyms: Gm16636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435900
Igkv3-3
Name: immunoglobulin kappa variable 3-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 667946
RP23-454P8.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 74,746,563 bp
  • A to G, chromosome 2 at 106,372,353 bp
  • T to C, chromosome 2 at 143,696,700 bp
  • T to C, chromosome 3 at 79,087,866 bp
  • A to G, chromosome 3 at 89,173,478 bp
  • A to G, chromosome 4 at 11,289,700 bp
  • G to A, chromosome 4 at 43,257,936 bp
  • A to G, chromosome 4 at 45,249,196 bp
  • A to T, chromosome 4 at 99,031,022 bp
  • A to G, chromosome 4 at 107,200,462 bp
  • A to G, chromosome 4 at 109,422,915 bp
  • T to C, chromosome 5 at 3,473,102 bp
  • G to A, chromosome 5 at 33,410,908 bp
  • T to A, chromosome 5 at 109,221,288 bp
  • G to A, chromosome 5 at 128,743,880 bp
  • T to C, chromosome 5 at 130,269,390 bp
  • A to T, chromosome 6 at 39,464,293 bp
  • A to G, chromosome 6 at 68,571,094 bp
  • G to C, chromosome 6 at 70,687,430 bp
  • G to A, chromosome 6 at 90,662,878 bp
  • T to C, chromosome 7 at 28,396,615 bp
  • A to G, chromosome 7 at 46,850,896 bp
  • A to G, chromosome 7 at 86,412,467 bp
  • C to A, chromosome 7 at 103,418,556 bp
  • A to T, chromosome 7 at 140,202,722 bp
  • A to T, chromosome 7 at 143,080,968 bp
  • T to A, chromosome 8 at 107,555,078 bp
  • T to A, chromosome 9 at 64,168,610 bp
  • T to C, chromosome 9 at 65,177,515 bp
  • T to C, chromosome 10 at 77,069,476 bp
  • A to G, chromosome 10 at 127,588,067 bp
  • T to C, chromosome 11 at 45,886,480 bp
  • T to A, chromosome 11 at 49,020,377 bp
  • A to T, chromosome 11 at 49,318,413 bp
  • G to T, chromosome 11 at 52,374,967 bp
  • A to G, chromosome 11 at 57,217,794 bp
  • A to G, chromosome 11 at 59,275,356 bp
  • T to A, chromosome 13 at 36,204,562 bp
  • A to G, chromosome 13 at 93,391,779 bp
  • A to G, chromosome 14 at 67,764,655 bp
  • A to T, chromosome 15 at 47,838,435 bp
  • A to T, chromosome 15 at 77,807,986 bp
  • C to A, chromosome 17 at 25,816,843 bp
  • G to T, chromosome 17 at 38,335,624 bp
  • A to C, chromosome 17 at 39,040,002 bp
  • C to T, chromosome 18 at 63,022,350 bp
  • T to C, chromosome 19 at 11,984,491 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5413 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042982-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.