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Strain Name:
C57BL/6J-MtgxR5426Btlr/Mmmh
Stock Number:
042992-MU
Citation ID:
RRID:MMRRC_042992-MU
Other Names:
R5426 (G1), C57BL/6J-MtgxR5426Btlr
Major Collection:

Strain Information

Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Myd88
Name: myeloid differentiation primary response gene 88
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17874
HGNC: HGNC:7562
Homologene: 1849
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Chgb
Name: chromogranin B
Synonyms: secretogranin I, Scg-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12653
HGNC: HGNC:1930
Homologene: 1375
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Kifap3
Name: kinesin-associated protein 3
Synonyms: KAP3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16579
Homologene: 7799
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 23,849,390 bp
  • A to G, chromosome 1 at 37,115,671 bp
  • T to C, chromosome 1 at 46,242,206 bp
  • T to A, chromosome 1 at 58,666,886 bp
  • A to G, chromosome 1 at 163,779,871 bp
  • C to T, chromosome 2 at 31,336,544 bp
  • A to G, chromosome 2 at 32,609,256 bp
  • T to C, chromosome 2 at 89,609,739 bp
  • T to A, chromosome 2 at 90,252,611 bp
  • T to A, chromosome 2 at 101,898,875 bp
  • A to T, chromosome 2 at 132,793,533 bp
  • C to A, chromosome 2 at 152,538,948 bp
  • C to A, chromosome 2 at 180,471,235 bp
  • T to C, chromosome 3 at 66,220,722 bp
  • T to C, chromosome 3 at 131,341,797 bp
  • T to A, chromosome 4 at 28,821,427 bp
  • A to G, chromosome 4 at 43,770,168 bp
  • T to A, chromosome 4 at 88,629,410 bp
  • C to T, chromosome 4 at 97,690,670 bp
  • C to A, chromosome 4 at 123,322,713 bp
  • A to G, chromosome 4 at 141,623,566 bp
  • A to G, chromosome 5 at 4,888,975 bp
  • A to T, chromosome 5 at 62,642,816 bp
  • T to C, chromosome 5 at 87,850,885 bp
  • G to C, chromosome 5 at 137,667,758 bp
  • A to G, chromosome 5 at 143,715,852 bp
  • A to G, chromosome 6 at 40,386,867 bp
  • A to G, chromosome 6 at 83,976,414 bp
  • T to G, chromosome 6 at 85,707,823 bp
  • G to A, chromosome 6 at 90,559,299 bp
  • G to A, chromosome 6 at 129,641,550 bp
  • C to A, chromosome 6 at 135,732,368 bp
  • T to C, chromosome 7 at 23,418,201 bp
  • T to C, chromosome 7 at 26,187,655 bp
  • T to A, chromosome 7 at 43,464,823 bp
  • A to G, chromosome 7 at 44,509,829 bp
  • C to A, chromosome 7 at 106,752,566 bp
  • T to C, chromosome 7 at 108,322,810 bp
  • T to C, chromosome 7 at 108,755,717 bp
  • G to A, chromosome 7 at 135,268,647 bp
  • C to T, chromosome 8 at 54,681,399 bp
  • T to A, chromosome 9 at 25,286,690 bp
  • T to C, chromosome 9 at 42,986,584 bp
  • T to A, chromosome 9 at 59,297,549 bp
  • G to C, chromosome 9 at 119,339,750 bp
  • T to A, chromosome 9 at 124,294,003 bp
  • C to A, chromosome 10 at 10,350,260 bp
  • G to T, chromosome 10 at 40,290,187 bp
  • T to C, chromosome 10 at 75,267,550 bp
  • T to A, chromosome 11 at 9,290,722 bp
  • C to T, chromosome 11 at 30,506,354 bp
  • A to G, chromosome 11 at 49,059,256 bp
  • A to G, chromosome 11 at 50,421,734 bp
  • A to G, chromosome 11 at 56,068,907 bp
  • A to G, chromosome 11 at 58,125,287 bp
  • A to C, chromosome 11 at 75,450,896 bp
  • A to G, chromosome 11 at 100,464,116 bp
  • A to T, chromosome 11 at 103,620,760 bp
  • A to T, chromosome 12 at 40,745,745 bp
  • T to A, chromosome 13 at 58,563,154 bp
  • A to T, chromosome 13 at 67,257,192 bp
  • G to A, chromosome 13 at 113,369,053 bp
  • T to A, chromosome 14 at 68,120,066 bp
  • G to A, chromosome 14 at 78,498,864 bp
  • A to G, chromosome 15 at 7,922,105 bp
  • G to A, chromosome 15 at 40,148,536 bp
  • G to A, chromosome 15 at 40,148,537 bp
  • T to C, chromosome 15 at 75,982,096 bp
  • T to A, chromosome 15 at 98,806,466 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • A to T, chromosome 16 at 34,262,653 bp
  • A to T, chromosome 16 at 36,709,619 bp
  • G to T, chromosome 16 at 59,098,302 bp
  • G to T, chromosome 16 at 89,865,392 bp
  • T to A, chromosome 17 at 23,899,313 bp
  • T to A, chromosome 17 at 33,019,595 bp
  • C to T, chromosome 17 at 78,773,713 bp
  • T to A, chromosome 17 at 78,841,234 bp
  • A to C, chromosome 17 at 86,498,733 bp
  • C to T, chromosome 18 at 61,003,236 bp
  • C to T, chromosome 19 at 11,325,802 bp
  • G to A, chromosome 19 at 40,183,957 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5426 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042992-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.