Strain Name:
C57BL/6J-MtgxR5427Btlr/Mmmh
Stock Number:
042993-MU
Citation ID:
RRID:MMRRC_042993-MU
Other Names:
R5427 (G1), C57BL/6J-MtgxR5427Btlr
Major Collection:

Gene Information

Npy5r
Name: neuropeptide Y receptor Y5
Synonyms: Y5R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18168
HGNC: HGNC:7958
Homologene: 21241
Crym
Name: crystallin, mu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12971
HGNC: HGNC:2418
Homologene: 1424
Bbx
Name: bobby sox HMG box containing
Synonyms: 5530401J07Rik, 5730403O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70508
Homologene: 10634
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: Myosin IIB, nonmuscle myosin heavy chain IIB, Fltn, Myhn-2, SMemb, NMHC-B, nonmuscle myosin heavy chain II-B, 5730504C04Rik, 9330167F11Rik, Fltn, myosin IIB, NMHC II-B, Myhn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Diaph1
Name: diaphanous related formin 1
Synonyms: D18Wsu154e, Diap1, Dia1, mDia1, Drf1, p140mDia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13367
HGNC: HGNC:2876
Homologene: 129567
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240725
Homologene: 49408
Akap13
Name: A kinase (PRKA) anchor protein 13
Synonyms: PROTO-LBC, 1700026G02Rik, 5730522G15Rik, 5830460E08Rik, PROTO-LB, Ht31, AKAP-Lbc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Nampt
Name: nicotinamide phosphoribosyltransferase
Synonyms: Visfatin, Pbef1, 1110035O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 59027
VEGA: 12
Homologene: 4201
Git2
Name: GIT ArfGAP 2
Synonyms: Cat-2, 5830420E16Rik, Cool associated tyrosine phosphorylated-2, ARF GTPase activating protein 2, 1500036H07Rik, B230104M05Rik, 9630056M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26431
HGNC: HGNC:4273
Homologene: 41336
Stim2
Name: stromal interaction molecule 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 116873
Homologene: 32490
Cep135
Name: centrosomal protein 135
Synonyms: LOC381644, Cep4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381644
Homologene: 45709
Csf2rb2
Name: colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Synonyms: AIC2A, BetaIl3, Bil3, Il3r, Il3rb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12984
VEGA: 15
HGNC: HGNC:2436
Homologene: 339
Cryab
Name: crystallin, alpha B
Synonyms: Crya2, alpha B-crystallin, Crya-2, HspB5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12955
VEGA: 9
HGNC: HGNC:2389
Homologene: 68209
Pfas
Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms: Sofa, 4432409B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237823
HGNC: HGNC:8863
Homologene: 5970
Anp32a
Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Synonyms: Anp32, pp32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11737
Homologene: 137229
Adgrg5
Name: adhesion G protein-coupled receptor G5
Synonyms: LOC382045, PGR27, Gpr114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382045
Homologene: 17828
Gclm
Name: glutamate-cysteine ligase, modifier subunit
Synonyms: Gcmc, Glclr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14630
HGNC: HGNC:4312
Homologene: 1557
Pi4kb
Name: phosphatidylinositol 4-kinase beta
Synonyms: Pik4cb, ESTM41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 107650
HGNC: HGNC:8984
Homologene: 6741
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, Odz3, 2610100B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Timm23
Name: translocase of inner mitochondrial membrane 23
Synonyms: Tim23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 53600
Homologene: 9651
Palld
Name: palladin, cytoskeletal associated protein
Synonyms: 2410003B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72333
Homologene: 75052
Zbtb48
Name: zinc finger and BTB domain containing 48
Synonyms: 0610011D15Rik, Hkr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100090
HGNC: HGNC:4930
Homologene: 3905
Fign
Name: fidgetin
Synonyms: Fgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 60344
Homologene: 5813
Eci3
Name: enoyl-Coenzyme A delta isomerase 3
Synonyms: 1810022C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69123
Homologene: 74357
Pcsk6
Name: proprotein convertase subtilisin/kexin type 6
Synonyms: PACE4, b2b2830Clo, SPC4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18553
HGNC: HGNC:8569
Homologene: 20569
Atg4b
Name: autophagy related 4B, cysteine peptidase
Synonyms: 2510009N07Rik, Apg4b, autophagin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66615
Homologene: 100868
Catsperg2
Name: cation channel sperm associated auxiliary subunit gamma 2
Synonyms: CATSPERG, 1700067C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76718
Homologene: 10915
Alpi
Name: alkaline phosphatase, intestinal
Synonyms: 2010001C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76768
Homologene: 122414
Myt1l
Name: myelin transcription factor 1-like
Synonyms: 2900093J19Rik, Png-1, 2900046C06Rik, C630034G21Rik, Nztf1, Pmng1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17933
VEGA: 12
HGNC: HGNC:7623
Homologene: 7435
Myom2
Name: myomesin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17930
HGNC: HGNC:7614
Homologene: 2953
Sppl2c
Name: signal peptide peptidase 2C
Synonyms: 4933407P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237958
Homologene: 18491
Rgs1
Name: regulator of G-protein signaling 1
Synonyms: BL34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 50778
HGNC: HGNC:9991
Homologene: 2191
Sntb1
Name: syntrophin, basic 1
Synonyms: beta1-Syntrophin, 59-1 DAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20649
Homologene: 9618
Slc22a27
Name: solute carrier family 22, member 27
Synonyms: mOAT6 related protein, AB056442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 171405
Homologene: 77136
Ankrd34a
Name: ankyrin repeat domain 34A
Synonyms: Ankrd34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 545554
Homologene: 82882
Tssk2
Name: testis-specific serine kinase 2
Synonyms: DGS-G, Tsk2, Stk22b, SPOGA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22115
VEGA: 16
Homologene: 31512
Zfp788
Name: zinc finger protein 788
Synonyms: 2810426N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67607
Homologene: 137363
Ccdc158
Name: coiled-coil domain containing 158
Synonyms: 4932413O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320696
Homologene: 18560
Adcy7
Name: adenylate cyclase 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11513
HGNC: HGNC:238
Homologene: 866
Olfr1487
Name: olfactory receptor 1487
Synonyms: MOR202-18, GA_x6K02T2RE5P-3951719-3952666
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258629
Homologene: 110492
Kcnk3
Name: potassium channel, subfamily K, member 3
Synonyms: Task-1, cTBAK-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16527
HGNC: HGNC:6278
Homologene: 1692
Vmn2r28
Name: vomeronasal 2, receptor 28
Synonyms: EG665255
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 665255
Homologene: 129605
Galk2
Name: galactokinase 2
Synonyms: 2810017M24Rik, Gk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69976
HGNC: HGNC:4119
Homologene: 13654
Nid1
Name: nidogen 1
Synonyms: entactin 1, entactin-1, nidogen-1, entactin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18073
VEGA: 13
HGNC: HGNC:7821
Homologene: 1878
Zfp709
Name: zinc finger protein 709
Synonyms: GIOT-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 236193
Homologene: 136795
Pnpo
Name: pyridoxine 5'-phosphate oxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103711
Homologene: 5364
Plod3
Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms: LH3, lysyl hydroxylase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26433
HGNC: HGNC:9083
Homologene: 843
1700057G04Rik
Name: RIKEN cDNA 1700057G04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78459
Homologene: 134508
Aaas
Name: achalasia, adrenocortical insufficiency, alacrimia
Synonyms: Aladin, GL003, D030041N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223921
VEGA: 15
Homologene: 9232
Fam89b
Name: family with sequence similarity 89, member B
Synonyms: 1110021A21Rik, Fam89b, Mtvr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17826
Homologene: 18749
Iqcf3
Name: IQ motif containing F3
Synonyms: 1700012F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68265
Homologene: 86789
Rrnad1
Name: ribosomal RNA adenine dimethylase domain containing 1
Synonyms: BC023814
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229503
Homologene: 9332
Erg28
Name: ergosterol biosynthesis 28
Synonyms: ORF11, 0610007P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 58520
HGNC: HGNC:1187
Homologene: 38284
Gm20422
Name: predicted gene 20422
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Anapc15
Name: anaphase promoting complex C subunit 15
Synonyms: 6330414C15Rik, 3200002M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75430
Homologene: 87047
Gm38394
Name: predicted gene, 38394
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Olfr1116
Name: olfactory receptor 1116
Synonyms: MOR264-21P, MOR264-8P, Olfr1521-ps1, Olfr1116-ps, GA_x6K02T2Q125-48753766-48754734
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257875
Homologene: 134077
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 12,796,912 bp
  • T to C, chromosome 1 at 87,101,354 bp
  • G to C, chromosome 1 at 93,775,206 bp
  • A to G, chromosome 1 at 133,657,595 bp
  • A to T, chromosome 1 at 144,246,280 bp
  • A to G, chromosome 2 at 63,978,998 bp
  • A to T, chromosome 2 at 87,269,514 bp
  • T to C, chromosome 2 at 125,946,821 bp
  • T to C, chromosome 3 at 87,924,332 bp
  • A to G, chromosome 3 at 94,994,207 bp
  • G to A, chromosome 3 at 96,597,521 bp
  • T to C, chromosome 3 at 122,266,327 bp
  • A to G, chromosome 4 at 152,020,651 bp
  • A to G, chromosome 5 at 30,622,295 bp
  • A to T, chromosome 5 at 54,110,939 bp
  • T to A, chromosome 5 at 76,638,202 bp
  • T to C, chromosome 5 at 92,648,962 bp
  • A to G, chromosome 5 at 114,730,328 bp
  • A to T, chromosome 5 at 136,991,788 bp
  • A to T, chromosome 7 at 5,486,377 bp
  • T to C, chromosome 7 at 29,714,850 bp
  • G to A, chromosome 7 at 41,649,652 bp
  • C to T, chromosome 7 at 66,033,899 bp
  • A to G, chromosome 7 at 75,728,869 bp
  • C to T, chromosome 7 at 101,898,603 bp
  • A to C, chromosome 7 at 120,199,222 bp
  • C to A, chromosome 8 at 15,113,764 bp
  • A to G, chromosome 8 at 48,236,564 bp
  • C to T, chromosome 8 at 61,550,072 bp
  • G to A, chromosome 8 at 66,681,020 bp
  • A to G, chromosome 8 at 69,743,456 bp
  • A to G, chromosome 8 at 71,889,132 bp
  • G to A, chromosome 8 at 88,326,201 bp
  • A to T, chromosome 8 at 94,935,102 bp
  • A to G, chromosome 9 at 50,756,293 bp
  • A to G, chromosome 9 at 62,377,316 bp
  • G to A, chromosome 9 at 92,352,596 bp
  • T to C, chromosome 9 at 106,543,860 bp
  • T to C, chromosome 11 at 68,802,931 bp
  • T to C, chromosome 11 at 69,001,153 bp
  • T to C, chromosome 11 at 96,943,807 bp
  • G to A, chromosome 11 at 104,187,867 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • A to T, chromosome 12 at 32,834,915 bp
  • T to C, chromosome 12 at 85,819,567 bp
  • A to G, chromosome 13 at 13,483,683 bp
  • G to T, chromosome 13 at 34,959,948 bp
  • G to A, chromosome 14 at 32,189,146 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • T to A, chromosome 15 at 78,288,911 bp
  • A to G, chromosome 15 at 102,339,950 bp
  • A to G, chromosome 16 at 17,898,865 bp
  • T to C, chromosome 16 at 50,280,497 bp
  • A to T, chromosome 18 at 37,890,595 bp
  • A to G, chromosome 19 at 5,728,791 bp
  • A to G, chromosome 19 at 7,879,388 bp
  • A to T, chromosome 19 at 13,619,350 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5427 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042993-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.