Strain Name:
C57BL/6J-MtgxR5433Btlr/Mmmh
Stock Number:
042998-MU
Citation ID:
RRID:MMRRC_042998-MU
Other Names:
R5433 (G1), C57BL/6J-MtgxR5433Btlr
Major Collection:

Strain Information

Cd74
Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Synonyms: CLIP, Ii
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16149
VEGA: 18
HGNC: HGNC:1697
Homologene: 3209
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Lars
Name: leucyl-tRNA synthetase
Synonyms: 3110009L02Rik, 2310045K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Gon4l
Name: gon-4-like (C.elegans)
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76022
Homologene: 13002
Mapkapk3
Name: mitogen-activated protein kinase-activated protein kinase 3
Synonyms: MK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102626
HGNC: HGNC:6888
Homologene: 55836
Rrm1
Name: ribonucleotide reductase M1
Synonyms: RnrM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20133
Homologene: 806
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Tcof1
Name: treacle ribosome biogenesis factor 1
Synonyms: treacle
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 21453
Homologene: 68049
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64898
Homologene: 8769
1700019D03Rik
Name: RIKEN cDNA 1700019D03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67080
Homologene: 49994
Lrrfip1
Name: leucine rich repeat (in FLII) interacting protein 1
Synonyms: FLAP (FLI LRR associated protein), Fliiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16978
HGNC: HGNC:6702
Homologene: 48301
Slc22a3
Name: solute carrier family 22 (organic cation transporter), member 3
Synonyms: EMT, Oct3, Orct3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20519
VEGA: 17
Homologene: 22630
Ptprm
Name: protein tyrosine phosphatase, receptor type, M
Synonyms: RPTPmu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19274
VEGA: 17
HGNC: HGNC:9675
Homologene: 37694
Btnl9
Name: butyrophilin-like 9
Synonyms: D330012D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237754
Homologene: 68540
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: per, Pcn, Plc, perlecan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Elp6
Name: elongator acetyltransferase complex subunit 6
Synonyms: 2610001P13Rik, Tmem103, 2610002I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72341
Homologene: 9794
Morc2a
Name: microrchidia 2A
Synonyms: 8430403M08Rik, Zcwcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74522
Homologene: 8966
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225115
Homologene: 25090
Rprd1a
Name: regulation of nuclear pre-mRNA domain containing 1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225283
VEGA: 18
Homologene: 124432
Szt2
Name: SZT2 subunit of KICSTOR complex
Synonyms: seaizure threshold 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230676
Homologene: 49413
Lamtor5
Name: late endosomal/lysosomal adaptor, MAPK and MTOR activator 5
Synonyms: 1110003H18Rik, Hbxip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68576
Homologene: 4668
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, Myhc-a, alpha cardiac MHC, cardiomyopathy, hypertrophic 1, Myhca, A830009F23Rik, alpha-MHC, alphaMHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Il1a
Name: interleukin 1 alpha
Synonyms: Il-1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16175
HGNC: HGNC:5991
Homologene: 480
Gvin3
Name: GTPase, very large interferon inducible, family member 3
Synonyms: Gm1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434223
Homologene: 32708
Gucy2d
Name: guanylate cyclase 2d
Synonyms: guanylyl cyclase D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14918
Homologene: 71540
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: 1110008G13Rik, LOC100042382, Liprin-alpha4, Gm3812
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
Rasal3
Name: RAS protein activator like 3
Synonyms: A430107D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320484
Homologene: 18901
1810064F22Rik
Name: RIKEN cDNA 1810064F22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69862
Tmem30a
Name: transmembrane protein 30A
Synonyms: 2010200I23Rik, D9Wsu20e, Cdc50a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69981
Homologene: 110703
Xkr7
Name: X-linked Kx blood group related 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228787
Homologene: 19390
Ces1d
Name: carboxylesterase 1D
Synonyms: TGH, Ces3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104158
HGNC: HGNC:1863
Homologene: 35606
Dynlt5
Name: dynein light chain Tctex-type 5
Synonyms: 1700055O19Rik, Tctex1d1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67344
Homologene: 12117
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A3, UT-A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
Shc4
Name: SHC (Src homology 2 domain containing) family, member 4
Synonyms: 9930029B02Rik, LOC271849, 6230417E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271849
Homologene: 19567
Cyp2c65
Name: cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms: 2210009K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72303
Homologene: 133566
Coro1b
Name: coronin, actin binding protein 1B
Synonyms: coronin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 23789
HGNC: HGNC:2253
Homologene: 40790
Svop
Name: SV2 related protein
Synonyms: 1110030H18Rik, msvop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68666
Homologene: 41283
Vmn2r16
Name: vomeronasal 2, receptor 16
Synonyms: EG384220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384220
Homologene: 104825
Il22b
Name: interleukin 22B
Synonyms: IL-TIFb, Iltifb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 116849
Homologene: 9669
Aacs
Name: acetoacetyl-CoA synthetase
Synonyms: 2210408B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 78894
Homologene: 11322
Klhl18
Name: kelch-like 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270201
Homologene: 14805
Zer1
Name: zyg-11 related, cell cycle regulator
Synonyms: C230075L19Rik, Zyg11bl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227693
Homologene: 4621
Ceacam3
Name: CEA cell adhesion molecule 3
Synonyms: cea12, EG384557, Psg24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384557
Homologene: 86971
Or2z2
Name: olfactory receptor family 2 subfamily Z member 2
Synonyms: MTPCR07, MOR281-1, GA_x6K02T2NKPP-957001-957948, Olfr30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18329
Homologene: 131139
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54667
Homologene: 23226
Rgs20
Name: regulator of G-protein signaling 20
Synonyms: Rgsz1, 2900073E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 58175
Homologene: 2745
Col10a1
Name: collagen, type X, alpha 1
Synonyms: Col10a-1, Col10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12813
HGNC: HGNC:2185
Homologene: 55466
Or1af1
Name: olfactory receptor family 1 subfamily AF member 1
Synonyms: GA_x6K02T2NLDC-33902472-33903401, MOR138-5P, MOR138-6, Olfr366
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 236509
Homologene: 114744
Adamts8
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8
Synonyms: METH2, METH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 30806
HGNC: HGNC:224
Homologene: 5108
Mknk2
Name: MAP kinase-interacting serine/threonine kinase 2
Synonyms: Mnk2, 2010016G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17347
HGNC: HGNC:7111
Homologene: 49674
Kcna1
Name: potassium voltage-gated channel, shaker-related subfamily, member 1
Synonyms: Kv1.1, mouse brain potassium channel protein-1, MBK1, Mk-1, Shak, mceph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16485
HGNC: HGNC:6218
Homologene: 183
Or1e16
Name: olfactory receptor family 1 subfamily E member 16
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54, Olfr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Guca1a
Name: guanylate cyclase activator 1a (retina)
Synonyms: Guca1, mGCAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14913
VEGA: 17
HGNC: HGNC:4678
Homologene: 353
Dio1
Name: deiodinase, iodothyronine, type I
Synonyms: D1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13370
HGNC: HGNC:2883
Homologene: 620
Sh2d6
Name: SH2 domain containing 6
Synonyms: 4933424C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71130
Homologene: 65986
Prok1
Name: prokineticin 1
Synonyms: EG-VEGF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 246691
Homologene: 13023
BC030500
Name: cDNA sequence BC030500
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234290
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 5,070,110 bp
  • T to C, chromosome 1 at 52,925,498 bp
  • T to G, chromosome 1 at 91,087,126 bp
  • A to T, chromosome 1 at 134,317,894 bp
  • A to G, chromosome 2 at 30,100,986 bp
  • T to G, chromosome 2 at 37,219,672 bp
  • T to A, chromosome 2 at 125,639,430 bp
  • G to T, chromosome 2 at 129,307,901 bp
  • T to A, chromosome 2 at 153,054,324 bp
  • A to T, chromosome 3 at 88,896,225 bp
  • T to C, chromosome 3 at 89,952,909 bp
  • T to G, chromosome 3 at 98,126,134 bp
  • T to A, chromosome 3 at 107,239,633 bp
  • T to G, chromosome 3 at 107,282,007 bp
  • A to G, chromosome 4 at 103,002,503 bp
  • A to T, chromosome 4 at 107,306,780 bp
  • A to T, chromosome 4 at 118,375,466 bp
  • T to A, chromosome 4 at 137,528,794 bp
  • G to T, chromosome 5 at 109,363,842 bp
  • A to G, chromosome 5 at 114,060,125 bp
  • A to T, chromosome 5 at 125,515,014 bp
  • A to G, chromosome 6 at 72,517,142 bp
  • A to T, chromosome 6 at 126,643,112 bp
  • G to A, chromosome 7 at 17,159,883 bp
  • G to A, chromosome 7 at 98,449,775 bp
  • A to T, chromosome 7 at 102,465,767 bp
  • T to A, chromosome 7 at 106,600,107 bp
  • A to G, chromosome 8 at 58,913,009 bp
  • G to A, chromosome 8 at 93,186,036 bp
  • A to G, chromosome 9 at 22,207,743 bp
  • A to C, chromosome 9 at 30,961,716 bp
  • T to A, chromosome 9 at 79,780,648 bp
  • T to C, chromosome 9 at 107,256,292 bp
  • A to G, chromosome 9 at 110,315,783 bp
  • G to C, chromosome 9 at 110,436,127 bp
  • A to G, chromosome 10 at 34,390,739 bp
  • A to T, chromosome 10 at 80,667,225 bp
  • T to A, chromosome 10 at 118,294,884 bp
  • T to C, chromosome 11 at 3,680,823 bp
  • T to A, chromosome 11 at 49,176,003 bp
  • C to T, chromosome 11 at 58,455,854 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • A to G, chromosome 14 at 54,953,924 bp
  • C to T, chromosome 17 at 12,458,490 bp
  • C to T, chromosome 17 at 32,393,601 bp
  • T to C, chromosome 17 at 47,400,370 bp
  • A to T, chromosome 17 at 66,693,473 bp
  • T to C, chromosome 17 at 71,225,053 bp
  • A to G, chromosome 18 at 5,059,294 bp
  • T to C, chromosome 18 at 24,507,231 bp
  • A to G, chromosome 18 at 42,251,298 bp
  • G to A, chromosome 18 at 49,867,899 bp
  • G to T, chromosome 18 at 60,807,921 bp
  • T to C, chromosome 18 at 60,818,033 bp
  • G to T, chromosome 18 at 78,208,928 bp
  • C to T, chromosome 19 at 4,153,450 bp
  • A to T, chromosome 19 at 39,093,484 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5433 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042998-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.