Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5435Btlr/Mmmh
Stock Number:
043000-MU
Citation ID:
RRID:MMRRC_043000-MU
Other Names:
R5435 (G1), C57BL/6J-MtgxR5435Btlr
Major Collection:

Strain Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Col2a-1, Col2a, Col2, M100856, Rgsc856, Lpk, M100413, Rgsc413
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Prkar2a
Name: protein kinase, cAMP dependent regulatory, type II alpha
Synonyms: RII(alpha), 1110061A24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19087
HGNC: HGNC:9391
Homologene: 3064
Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Wee1
Name: WEE 1 homolog 1 (S. pombe)
Synonyms: Wee1A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22390
Homologene: 31152
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,546,453 bp
  • T to A, chromosome 1 at 82,454,007 bp
  • T to A, chromosome 1 at 105,741,250 bp
  • T to A, chromosome 1 at 132,494,275 bp
  • T to A, chromosome 2 at 66,273,534 bp
  • T to A, chromosome 2 at 67,396,152 bp
  • A to T, chromosome 2 at 76,914,358 bp
  • T to A, chromosome 2 at 85,400,470 bp
  • C to A, chromosome 3 at 93,443,672 bp
  • T to C, chromosome 3 at 95,297,250 bp
  • C to A, chromosome 3 at 95,622,458 bp
  • A to T, chromosome 4 at 43,493,519 bp
  • A to G, chromosome 4 at 81,283,487 bp
  • A to G, chromosome 4 at 94,686,813 bp
  • A to T, chromosome 4 at 101,606,610 bp
  • G to A, chromosome 5 at 49,990,126 bp
  • A to T, chromosome 5 at 69,563,169 bp
  • G to A, chromosome 5 at 103,754,332 bp
  • T to A, chromosome 5 at 137,403,762 bp
  • T to A, chromosome 6 at 58,855,806 bp
  • T to A, chromosome 6 at 73,060,138 bp
  • G to A, chromosome 6 at 113,151,221 bp
  • T to G, chromosome 6 at 139,715,855 bp
  • A to G, chromosome 7 at 18,478,473 bp
  • T to A, chromosome 7 at 45,156,272 bp
  • TCCCC to TCCC, chromosome 7 at 110,124,569 bp
  • A to G, chromosome 7 at 126,775,788 bp
  • T to A, chromosome 7 at 128,675,324 bp
  • G to A, chromosome 7 at 139,996,072 bp
  • T to C, chromosome 8 at 13,631,811 bp
  • T to A, chromosome 8 at 109,838,920 bp
  • T to C, chromosome 8 at 111,008,826 bp
  • G to A, chromosome 8 at 121,084,492 bp
  • T to A, chromosome 8 at 122,780,281 bp
  • G to T, chromosome 9 at 54,615,869 bp
  • A to G, chromosome 9 at 56,206,486 bp
  • A to T, chromosome 9 at 66,834,501 bp
  • A to G, chromosome 9 at 79,707,901 bp
  • A to G, chromosome 9 at 100,953,550 bp
  • A to G, chromosome 9 at 108,740,483 bp
  • A to G, chromosome 9 at 111,391,890 bp
  • C to A, chromosome 10 at 56,040,150 bp
  • C to T, chromosome 10 at 79,912,458 bp
  • A to G, chromosome 10 at 105,779,586 bp
  • G to A, chromosome 10 at 128,195,126 bp
  • T to A, chromosome 11 at 73,712,113 bp
  • T to C, chromosome 11 at 82,103,175 bp
  • A to G, chromosome 12 at 8,989,951 bp
  • A to G, chromosome 12 at 113,597,663 bp
  • A to T, chromosome 13 at 13,777,064 bp
  • A to G, chromosome 13 at 13,996,990 bp
  • A to G, chromosome 13 at 24,752,101 bp
  • C to A, chromosome 13 at 43,401,372 bp
  • A to G, chromosome 13 at 92,508,237 bp
  • A to T, chromosome 14 at 23,528,404 bp
  • A to G, chromosome 14 at 32,661,456 bp
  • A to G, chromosome 14 at 33,020,311 bp
  • A to G, chromosome 14 at 70,139,327 bp
  • C to T, chromosome 15 at 3,965,792 bp
  • C to T, chromosome 15 at 77,769,609 bp
  • T to C, chromosome 15 at 98,000,510 bp
  • G to T, chromosome 16 at 17,142,762 bp
  • C to T, chromosome 16 at 31,456,657 bp
  • A to T, chromosome 16 at 96,122,330 bp
  • C to T, chromosome 17 at 24,267,614 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • A to G, chromosome 17 at 35,425,685 bp
  • A to G, chromosome 18 at 36,993,599 bp
  • A to T, chromosome 18 at 86,398,263 bp
  • T to G, chromosome 19 at 8,820,318 bp
  • T to G, chromosome 19 at 25,411,143 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5435 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043000-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.