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Strain Name:
C57BL/6J-MtgxR5442Btlr/Mmmh
Stock Number:
043007-MU
Citation ID:
RRID:MMRRC_043007-MU
Other Names:
R5442 (G1), C57BL/6J-MtgxR5442Btlr
Major Collection:

Strain Information

Lmf1
Name: lipase maturation factor 1
Synonyms: 2400010G15Rik, Tmem112
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76483
Homologene: 23384
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 170750
Homologene: 6424
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Mcmbp
Name: minichromosome maintenance complex binding protein
Synonyms: 1110007A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210711
Homologene: 11749
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 87,718,066 bp
  • A to G, chromosome 1 at 165,513,140 bp
  • T to C, chromosome 2 at 35,903,123 bp
  • T to C, chromosome 2 at 86,041,951 bp
  • A to G, chromosome 2 at 122,486,869 bp
  • A to G, chromosome 2 at 152,628,057 bp
  • A to G, chromosome 2 at 160,905,016 bp
  • A to G, chromosome 2 at 178,142,461 bp
  • C to T, chromosome 3 at 37,309,945 bp
  • C to T, chromosome 3 at 82,903,220 bp
  • C to A, chromosome 4 at 57,637,876 bp
  • G to T, chromosome 4 at 65,581,786 bp
  • A to G, chromosome 4 at 139,407,772 bp
  • T to C, chromosome 5 at 101,896,559 bp
  • A to G, chromosome 5 at 138,265,780 bp
  • T to C, chromosome 6 at 12,748,800 bp
  • G to A, chromosome 6 at 125,312,794 bp
  • G to T, chromosome 7 at 10,633,950 bp
  • G to T, chromosome 7 at 86,920,122 bp
  • A to T, chromosome 7 at 89,144,664 bp
  • A to T, chromosome 7 at 104,975,228 bp
  • T to C, chromosome 7 at 128,712,660 bp
  • T to C, chromosome 7 at 140,922,190 bp
  • G to A, chromosome 7 at 141,240,937 bp
  • T to A, chromosome 9 at 38,349,862 bp
  • A to G, chromosome 9 at 57,156,806 bp
  • A to G, chromosome 10 at 5,343,473 bp
  • C to T, chromosome 10 at 79,912,458 bp
  • T to C, chromosome 10 at 116,918,848 bp
  • T to C, chromosome 10 at 118,860,738 bp
  • A to G, chromosome 11 at 98,712,559 bp
  • A to G, chromosome 11 at 98,821,471 bp
  • A to G, chromosome 11 at 120,018,768 bp
  • T to C, chromosome 15 at 27,856,194 bp
  • C to A, chromosome 15 at 77,489,087 bp
  • T to C, chromosome 15 at 97,758,861 bp
  • G to A, chromosome 17 at 25,588,669 bp
  • A to T, chromosome 17 at 37,499,439 bp
  • T to C, chromosome 18 at 36,939,862 bp
  • A to T, chromosome 18 at 61,857,225 bp
  • C to T, chromosome 19 at 53,012,238 bp
  • A to G, chromosome Y at 1,336,467 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5442 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043007-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.