Strain Name:
C57BL/6J-MtgxR5449Btlr/Mmmh
Stock Number:
043014-MU
Citation ID:
RRID:MMRRC_043014-MU
Other Names:
R5449 (G1), C57BL/6J-MtgxR5449Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Rgs9
Name: regulator of G-protein signaling 9
Synonyms: Rgs9-2, RGS9-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19739
Homologene: 2845
Faah
Name: fatty acid amide hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14073
HGNC: HGNC:3553
Homologene: 68184
Cfl1
Name: cofilin 1, non-muscle
Synonyms: cofilin, Cof, n-cofilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 12631
HGNC: HGNC:1874
Homologene: 99735
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15184
Homologene: 3995
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269800
Homologene: 15849
Wee1
Name: WEE 1 homolog 1 (S. pombe)
Synonyms: Wee1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22390
Homologene: 31152
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237877
Homologene: 32611
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Cep104
Name: centrosomal protein 104
Synonyms: BC046331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230967
Homologene: 44919
Plxna1
Name: plexin A1
Synonyms: NOV, Plxn1, 2600013D04Rik, PlexA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18844
HGNC: HGNC:9099
Homologene: 56426
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22222
Homologene: 7582
Cry1
Name: cryptochrome 1 (photolyase-like)
Synonyms: Phll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12952
VEGA: 10
HGNC: HGNC:2384
Homologene: 7042
Scamp2
Name: secretory carrier membrane protein 2
Synonyms: Sc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24044
VEGA: 9
Homologene: 4163
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214444
Homologene: 49533
Prpf40a
Name: pre-mRNA processing factor 40A
Synonyms: FBP11, 2810012K09Rik, Fnbp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56194
Homologene: 6377
Syn3
Name: synapsin III
Synonyms: Synapsin IIIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 27204
Homologene: 68320
Pgr
Name: progesterone receptor
Synonyms: NR3C3, PR, 9930019P03Rik, PR-B, PR-A, ENSMUSG00000074510
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Numb
Name: NUMB endocytic adaptor protein
Synonyms: m-numb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18222
HGNC: HGNC:8060
Homologene: 2775
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319262
Homologene: 14127
Riok3
Name: RIO kinase 3
Synonyms: 1200013N13Rik, Sudd, D18Ertd331e, E130306C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 66878
VEGA: 18
Homologene: 2843
Fchsd2
Name: FCH and double SH3 domains 2
Synonyms: Sh3md3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207278
Homologene: 8887
Slc7a5
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Synonyms: TA1, D0H16S474E, LAT1, Gm42049
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20539
Homologene: 55759
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20265
Homologene: 21375
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Dmpk
Name: dystrophia myotonica-protein kinase
Synonyms: DM, Dm15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13400
HGNC: HGNC:2933
Homologene: 3247
Nlrp9a
Name: NLR family, pyrin domain containing 9A
Synonyms: D7Ertd565e, Nalp9a, Nalp-theta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233001
Homologene: 116072
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Tro
Name: trophinin
Synonyms: necdin and trophinin like, Tnn, trophinin-2, magphinin-gamma, magphinin-beta 2, magphinin-alpha, magphinin, Maged3l, Maged3, Trol
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56191
Homologene: 75113
Hltf
Name: helicase-like transcription factor
Synonyms: P113, Snf2l3, Smarca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20585
Homologene: 30136
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69700
Homologene: 43567
Stk19
Name: serine/threonine kinase 19
Synonyms: G11, RP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54402
Homologene: 10449
Syne4
Name: spectrin repeat containing, nuclear envelope family member 4
Synonyms: 0610012K07Rik, AI428936, nesprin-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233066
Homologene: 17716
Vmn1r46
Name: vomeronasal 1 receptor 46
Synonyms: V1rb8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113856
Homologene: 134021
Vmn2r114
Name: vomeronasal 2, receptor 114
Synonyms: EG666002
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 666002
Homologene: 86604
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242409
Homologene: 72894
Pdc
Name: phosducin
Synonyms: Rpr-1, Pdc, Rpr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20028
HGNC: HGNC:8759
Homologene: 1950
Ilvbl
Name: ilvB (bacterial acetolactate synthase)-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216136
VEGA: 10
HGNC: HGNC:6041
Homologene: 68532
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75087
Homologene: 18988
4921539E11Rik
Name: RIKEN cDNA 4921539E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70941
Homologene: 52843
Cd44
Name: CD44 antigen
Synonyms: Ly-24, Pgp-1, HERMES
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12505
HGNC: HGNC:1681
Homologene: 508
Vmn1r120
Name: vomeronasal 1 receptor 120
Synonyms: Gm5730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435953
Homologene: 104166
A630010A05Rik
Name: RIKEN cDNA A630010A05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545280
Ldhc
Name: lactate dehydrogenase C
Synonyms: Ldh-3, Ldhc4, Ldh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16833
HGNC: HGNC:6544
Homologene: 22767
Ptrh1
Name: peptidyl-tRNA hydrolase 1 homolog
Synonyms: 2210013M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329384
Homologene: 6135
Nscme3l
Name: NSE3 homolog, SMC5-SMC6 complex component like
Synonyms: 1700020D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75555
Homologene: 137364
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 25,531,897 bp
  • A to C, chromosome 1 at 150,333,439 bp
  • A to G, chromosome 2 at 32,776,346 bp
  • G to T, chromosome 2 at 53,156,926 bp
  • T to A, chromosome 2 at 66,321,002 bp
  • T to C, chromosome 2 at 102,832,546 bp
  • T to A, chromosome 2 at 120,963,500 bp
  • A to G, chromosome 3 at 20,069,083 bp
  • T to C, chromosome 3 at 59,259,706 bp
  • CAAAG to CAAAGAAAG, chromosome 3 at 83,030,862 bp
  • G to A, chromosome 4 at 43,673,992 bp
  • A to C, chromosome 4 at 70,276,651 bp
  • T to A, chromosome 4 at 103,266,382 bp
  • T to C, chromosome 4 at 115,999,495 bp
  • T to A, chromosome 4 at 153,985,305 bp
  • A to G, chromosome 4 at 156,167,280 bp
  • T to C, chromosome 6 at 89,323,608 bp
  • T to C, chromosome 6 at 89,976,361 bp
  • T to C, chromosome 6 at 125,024,138 bp
  • A to G, chromosome 7 at 19,090,991 bp
  • A to C, chromosome 7 at 21,053,149 bp
  • A to G, chromosome 7 at 26,557,829 bp
  • T to C, chromosome 7 at 30,314,985 bp
  • T to C, chromosome 7 at 46,869,658 bp
  • T to C, chromosome 7 at 101,277,524 bp
  • TCCCC to TCCC, chromosome 7 at 110,124,569 bp
  • C to A, chromosome 8 at 121,886,348 bp
  • T to C, chromosome 9 at 8,956,343 bp
  • A to G, chromosome 9 at 57,580,871 bp
  • G to A, chromosome 10 at 78,577,028 bp
  • G to A, chromosome 10 at 85,133,135 bp
  • A to T, chromosome 10 at 86,351,570 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • A to G, chromosome 11 at 80,124,108 bp
  • G to A, chromosome 11 at 102,196,097 bp
  • T to C, chromosome 11 at 109,225,744 bp
  • T to C, chromosome 11 at 119,415,076 bp
  • A to G, chromosome 12 at 83,831,973 bp
  • T to C, chromosome 15 at 71,821,949 bp
  • A to G, chromosome 16 at 14,618,673 bp
  • ATTT to ATT, chromosome 17 at 23,290,932 bp
  • A to T, chromosome 17 at 34,836,738 bp
  • A to T, chromosome 18 at 12,155,246 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • A to G, chromosome 19 at 5,493,493 bp
  • G to A, chromosome 19 at 5,503,264 bp
  • T to C, chromosome X at 150,645,970 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5449 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043014-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.