Strain Name:
C57BL/6J-MtgxR5450Btlr/Mmmh
Stock Number:
043015-MU
Citation ID:
RRID:MMRRC_043015-MU
Other Names:
R5450 (G1), C57BL/6J-MtgxR5450Btlr
Major Collection:

Strain Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: D230017O13Rik, 2610103K11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: T-type Cav3.2, alpha13.2, Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67804
VEGA: 18
Homologene: 2332
Kcmf1
Name: potassium channel modulatory factor 1
Synonyms: 1700094M07Rik, Pmcf, clone DEBT-91
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74287
Homologene: 99845
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: Bat2l, 5830434P21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Rps6kb1
Name: ribosomal protein S6 kinase, polypeptide 1
Synonyms: S6K1, p70S6K1, p70/85s6k, 2610318I15Rik, p70s6k
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72508
Homologene: 81703
Atg14
Name: autophagy related 14
Synonyms: D14Ertd114e, D14Ertd436e, Barkor
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504663
VEGA: 14
Homologene: 45423
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240725
Homologene: 49408
Prpf40a
Name: pre-mRNA processing factor 40A
Synonyms: FBP11, Fnbp3, 2810012K09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56194
Homologene: 6377
Yars1
Name: tyrosyl-tRNA synthetase 1
Synonyms: Yars
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107271
Homologene: 2730
Rsf1
Name: remodeling and spacing factor 1
Synonyms: Hbxap, p325, XAP8, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Slitrk6
Name: SLIT and NTRK-like family, member 6
Synonyms: 4832410J21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239250
VEGA: 14
Homologene: 12986
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: 1500004I01Rik, 6430511D02Rik, Pir121
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76884
Homologene: 7936
Kif2c
Name: kinesin family member 2C
Synonyms: 4930402F02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73804
HGNC: HGNC:6393
Homologene: 21355
Slc30a5
Name: solute carrier family 30 (zinc transporter), member 5
Synonyms: Zntl1, 1810010K08Rik, ZTL1, ZnT-5, Znt5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69048
VEGA: 13
Homologene: 41503
Cd79a
Name: CD79A antigen (immunoglobulin-associated alpha)
Synonyms: Ly-54, Cd79a, Igalpha, Iga, Ig-alpha, mb-1, Ly54, Ig alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12518
HGNC: HGNC:1698
Homologene: 31053
Zbtb18
Name: zinc finger and BTB domain containing 18
Synonyms: Zfp238, RP58
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30928
Homologene: 21276
Clec4e
Name: C-type lectin domain family 4, member e
Synonyms: Mincle, Clecsf9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56619
Homologene: 22809
Pamr1
Name: peptidase domain containing associated with muscle regeneration 1
Synonyms: E430002G05Rik, RAMP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 210622
Homologene: 9134
Rps3
Name: ribosomal protein S3
Synonyms: D7Ertd795e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27050
Homologene: 779
Edrf1
Name: erythroid differentiation regulatory factor 1
Synonyms: 2700050L05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 214764
Homologene: 27985
Wdr75
Name: WD repeat domain 75
Synonyms: 2410118I19Rik, 1300003A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73674
Homologene: 32771
Eno4
Name: enolase 4
Synonyms: 6430537H07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226265
Homologene: 18691
Maml2
Name: mastermind like transcriptional coactivator 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270118
Homologene: 134147
Ppm1m
Name: protein phosphatase 1M
Synonyms: PP2C eta, 2810423O19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67905
Homologene: 44900
Eral1
Name: Era like 12S mitochondrial rRNA chaperone 1
Synonyms: 9130407C09Rik, 2610524P08Rik, MERA-W, MERA-S
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57837
HGNC: HGNC:3424
Homologene: 4167
Patl2
Name: protein associated with topoisomerase II homolog 2 (yeast)
Synonyms: 4930424G05Rik, Pat1a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67578
Homologene: 12155
Fam184b
Name: family with sequence similarity 184, member B
Synonyms: 9630031F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 58227
Homologene: 137353
Mx1
Name: MX dynamin-like GTPase 1
Synonyms: Mx-1, myxovirus (influenza) resistance 1 polypeptide, Mx
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17857
Slc4a3
Name: solute carrier family 4 (anion exchanger), member 3
Synonyms: A930038D23Rik, Ae3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20536
Homologene: 129474
Abcg4
Name: ATP binding cassette subfamily G member 4
Synonyms: 6430517O04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 192663
Homologene: 75179
Cyp2j11
Name: cytochrome P450, family 2, subfamily j, polypeptide 11
Synonyms: Cyp2j11-ps
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100066
HGNC: HGNC:2634
Homologene: 133819
Vmn1r73
Name: vomeronasal 1 receptor 73
Synonyms: V1rg2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171237
Homologene: 103842
Ces1f
Name: carboxylesterase 1F
Synonyms: TGH-2, CesML1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234564
HGNC: HGNC:1863
Homologene: 134311
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Ikzf3
Name: IKAROS family zinc finger 3
Synonyms: Zfpn1a3, Aiolos, 5830411O07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22780
Homologene: 8269
Lrguk
Name: leucine-rich repeats and guanylate kinase domain containing
Synonyms: 4921528H16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74354
Homologene: 34923
Vmn1r76
Name: vomeronasal 1 receptor 76
Synonyms: V1rg4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171239
Cd200r2
Name: Cd200 receptor 2
Synonyms: CD200 cell surface glycoprotein receptor isoform 2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 271375
Homologene: 45557
Mroh1
Name: maestro heat-like repeat family member 1
Synonyms: Heatr7a, D330001F17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223658
Homologene: 44882
Dsg1b
Name: desmoglein 1 beta
Synonyms: Dsg5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225256
HGNC: HGNC:3048
Homologene: 1463
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: Mll4, C430014K11Rik, Mll2, bapa
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Arhgef4
Name: Rho guanine nucleotide exchange factor 4
Synonyms: 9330140K16Rik, C230030N03Rik, Asef
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Zfp366
Name: zinc finger protein 366
Synonyms: DC-SCRIPT
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238803
Homologene: 17637
Ceacam20
Name: CEA cell adhesion molecule 20
Synonyms: 9130012D09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71601
Homologene: 19010
Rchy1
Name: ring finger and CHY zinc finger domain containing 1
Synonyms: Zfp363, 6720407C15Rik, PRO1996, Pirh2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68098
Homologene: 22894
Cacna2d4
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms: 5730412N02Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319734
Homologene: 26544
Itprid1
Name: ITPR interacting domain containing 1
Synonyms: Ccdc129, D530004J12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232016
Homologene: 52344
Ddx31
Name: DEAD/H box helicase 31
Synonyms: 5830444G11Rik, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227674
Homologene: 6389
Sardh
Name: sarcosine dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192166
Homologene: 5149
Fbxw13
Name: F-box and WD-40 domain protein 13
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 211305
Homologene: 110776
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242409
Homologene: 72894
Psg18
Name: pregnancy specific beta-1-glycoprotein 18
Synonyms: Cea-3, Cea3, mmCGM6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26438
HGNC: HGNC:9524
Homologene: 110989
Vmn1r47
Name: vomeronasal 1 receptor 47
Synonyms: V1ra4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113846
Homologene: 137651
Esp18
Name: exocrine gland secreted peptide 18
Synonyms: Gm20579
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100126774
VEGA: 17
Rxylt1
Name: ribitol xylosyltransferase 1
Synonyms: 6330415D21Rik, Tmem5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216395
Homologene: 8600
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75087
Homologene: 18988
Skint1
Name: selection and upkeep of intraepithelial T cells 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639781
Homologene: 87538
Panx2
Name: pannexin 2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 406218
HGNC: HGNC:8600
Homologene: 14155
Gtpbp6
Name: GTP binding protein 6 (putative)
Synonyms: Pgbpll, pgpl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 107999
Homologene: 8157
Cyp4f17
Name: cytochrome P450, family 4, subfamily f, polypeptide 17
Synonyms: EG208285
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 208285
VEGA: 17
Homologene: 129713
Catsperb
Name: cation channel sperm associated auxiliary subunit beta
Synonyms: 4932415G16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 271036
VEGA: 12
Homologene: 81904
Ces3a
Name: carboxylesterase 3A
Synonyms: Es-male carboxylesterase, Es31
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382053
HGNC: HGNC:1865
Homologene: 84407
Or4x6
Name: olfactory receptor family 4 subfamily X member 6
Synonyms: GA_x6K02T2Q125-51551618-51550689, MOR228-3, Olfr1269
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258339
Homologene: 133727
Speer4f2
Name: spermatogenesis associated glutamate (E)-rich protein 4f2
Synonyms: Gm3495, Gm3535
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041749
Cers1
Name: ceramide synthase 1
Synonyms: Lass1, to, Uog-1, CerS1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93898
Homologene: 128762
Slc29a2
Name: solute carrier family 29 (nucleoside transporters), member 2
Synonyms: HNP36, ENT2, Der12
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13340
VEGA: 19
Homologene: 37493
Tcstv7a
Name: Tcstv family member 7A
Synonyms: clone L5, AF067063, clone L2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 380878
Slc27a5
Name: solute carrier family 27 (fatty acid transporter), member 5
Synonyms: FACVL3, VLCSH2, FATP5, VLCS-H2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26459
Homologene: 7596
Gm13449
Name: predicted gene 13449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 118568511
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: Dnl1ll, G4.8, Dnase1ll, 2310005K03Rik
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Slc6a16
Name: solute carrier family 6, member 16
Synonyms: LOC381884
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381884
Homologene: 49366
Ighg1
Name: immunoglobulin heavy constant gamma 1 (G1m marker)
Synonyms: IgG1, Igh-4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16017
Rpl39-ps
Name: ribosomal protein L39, pseudogene
Synonyms: Gm15430, ENSMUSG00000036305
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100503815
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 12,796,907 bp
  • T to G, chromosome 1 at 34,807,324 bp
  • C to A, chromosome 1 at 45,812,164 bp
  • C to A, chromosome 1 at 75,552,656 bp
  • T to C, chromosome 1 at 177,447,205 bp
  • G to T, chromosome 2 at 27,239,698 bp
  • T to C, chromosome 2 at 28,886,969 bp
  • T to C, chromosome 2 at 32,219,671 bp
  • T to C, chromosome 2 at 35,013,038 bp
  • G to T, chromosome 2 at 53,156,926 bp
  • A to G, chromosome 2 at 90,118,669 bp
  • T to G, chromosome 2 at 102,639,317 bp
  • A to G, chromosome 2 at 122,125,281 bp
  • T to C, chromosome 3 at 64,126,590 bp
  • G to A, chromosome 4 at 43,673,992 bp
  • C to A, chromosome 4 at 96,339,876 bp
  • G to A, chromosome 4 at 112,025,532 bp
  • T to C, chromosome 4 at 117,178,223 bp
  • A to G, chromosome 4 at 129,197,246 bp
  • T to A, chromosome 5 at 17,373,219 bp
  • C to T, chromosome 5 at 45,539,801 bp
  • A to G, chromosome 5 at 91,953,088 bp
  • A to G, chromosome 5 at 110,107,125 bp
  • A to T, chromosome 6 at 34,071,061 bp
  • T to A, chromosome 6 at 55,968,811 bp
  • A to T, chromosome 6 at 72,842,930 bp
  • T to C, chromosome 6 at 90,022,213 bp
  • C to T, chromosome 6 at 119,236,666 bp
  • T to C, chromosome 6 at 123,285,537 bp
  • C to A, chromosome 7 at 11,756,449 bp
  • T to C, chromosome 7 at 11,930,684 bp
  • T to C, chromosome 7 at 12,994,942 bp
  • T to A, chromosome 7 at 18,353,425 bp
  • A to G, chromosome 7 at 19,978,208 bp
  • G to T, chromosome 7 at 24,899,262 bp
  • C to T, chromosome 7 at 45,261,248 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • A to T, chromosome 7 at 99,482,674 bp
  • G to T, chromosome 7 at 133,658,610 bp
  • T to C, chromosome 8 at 70,318,297 bp
  • C to A, chromosome 8 at 93,265,795 bp
  • G to A, chromosome 8 at 105,057,918 bp
  • A to C, chromosome 9 at 13,706,467 bp
  • C to G, chromosome 9 at 44,279,625 bp
  • A to G, chromosome 9 at 106,196,842 bp
  • A to C, chromosome 9 at 109,184,157 bp
  • T to G, chromosome 10 at 11,212,330 bp
  • C to T, chromosome 10 at 122,088,995 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • A to G, chromosome 11 at 46,284,252 bp
  • C to T, chromosome 11 at 78,078,357 bp
  • A to T, chromosome 11 at 86,532,837 bp
  • C to T, chromosome 11 at 98,467,086 bp
  • A to T, chromosome 12 at 101,446,068 bp
  • A to T, chromosome 12 at 113,330,506 bp
  • A to G, chromosome 13 at 99,229,585 bp
  • C to T, chromosome 13 at 100,821,172 bp
  • A to T, chromosome 13 at 119,828,363 bp
  • T to C, chromosome 14 at 47,551,464 bp
  • T to A, chromosome 14 at 110,750,097 bp
  • C to A, chromosome 15 at 76,432,347 bp
  • A to G, chromosome 15 at 89,068,959 bp
  • C to A, chromosome 15 at 98,855,086 bp
  • A to G, chromosome 15 at 102,635,148 bp
  • T to A, chromosome 16 at 44,909,571 bp
  • A to T, chromosome 16 at 97,454,147 bp
  • A to T, chromosome 17 at 25,383,186 bp
  • A to G, chromosome 17 at 32,528,886 bp
  • G to T, chromosome 17 at 39,408,179 bp
  • A to C, chromosome 18 at 20,409,064 bp
  • A to G, chromosome 18 at 53,210,712 bp
  • A to G, chromosome 19 at 5,029,275 bp
  • T to C, chromosome 19 at 58,960,247 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5450 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043015-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.