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Strain Name:
C57BL/6J-MtgxR5457Btlr/Mmmh
Stock Number:
043020-MU
Citation ID:
RRID:MMRRC_043020-MU
Other Names:
R5457 (G1), C57BL/6J-MtgxR5457Btlr
Major Collection:

Strain Information

Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Mcoln3
Name: mucolipin 3
Synonyms: varitint-waddler, Va, 6720490O21Rik, TRPML3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171166
Homologene: 10118
Tal1
Name: T cell acute lymphocytic leukemia 1
Synonyms: SCL/tal-1, Scl, bHLHa17, Hpt
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21349
Homologene: 2400
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Drosha
Name: drosha, ribonuclease type III
Synonyms: 1110013A17Rik, Etohi2, Rnasen
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14000
Homologene: 8293
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 80,524,064 bp
  • T to C, chromosome 1 at 87,775,091 bp
  • T to TN, chromosome 1 at 88,266,525 bp
  • T to A, chromosome 1 at 123,411,810 bp
  • T to C, chromosome 1 at 170,912,157 bp
  • G to T, chromosome 1 at 172,130,678 bp
  • A to G, chromosome 1 at 173,183,613 bp
  • C to T, chromosome 1 at 173,270,574 bp
  • C to T, chromosome 1 at 174,217,193 bp
  • G to A, chromosome 1 at 193,325,994 bp
  • C to T, chromosome 2 at 18,698,979 bp
  • A to T, chromosome 2 at 26,440,268 bp
  • A to G, chromosome 2 at 31,931,985 bp
  • A to T, chromosome 2 at 64,917,098 bp
  • A to G, chromosome 2 at 75,854,252 bp
  • T to C, chromosome 2 at 76,710,921 bp
  • A to T, chromosome 2 at 76,945,955 bp
  • G to T, chromosome 2 at 156,164,847 bp
  • G to A, chromosome 2 at 158,635,998 bp
  • A to T, chromosome 2 at 181,997,608 bp
  • T to A, chromosome 3 at 146,128,122 bp
  • A to G, chromosome 4 at 89,307,154 bp
  • T to C, chromosome 4 at 108,626,735 bp
  • T to C, chromosome 4 at 115,068,580 bp
  • A to G, chromosome 4 at 149,131,637 bp
  • C to T, chromosome 5 at 14,676,143 bp
  • A to T, chromosome 5 at 73,531,565 bp
  • A to T, chromosome 5 at 95,956,971 bp
  • A to G, chromosome 5 at 144,849,977 bp
  • T to C, chromosome 5 at 145,201,359 bp
  • T to C, chromosome 6 at 41,303,421 bp
  • A to G, chromosome 6 at 70,386,705 bp
  • G to T, chromosome 6 at 122,049,729 bp
  • G to T, chromosome 6 at 124,715,706 bp
  • T to A, chromosome 6 at 131,221,889 bp
  • A to T, chromosome 6 at 137,512,177 bp
  • T to C, chromosome 7 at 10,047,946 bp
  • T to G, chromosome 7 at 29,529,507 bp
  • T to A, chromosome 7 at 39,363,485 bp
  • A to T, chromosome 7 at 89,930,443 bp
  • T to C, chromosome 7 at 110,312,830 bp
  • A to G, chromosome 7 at 139,101,026 bp
  • A to C, chromosome 8 at 85,512,338 bp
  • C to A, chromosome 8 at 86,509,844 bp
  • T to C, chromosome 8 at 88,311,021 bp
  • T to A, chromosome 8 at 123,489,968 bp
  • A to T, chromosome 9 at 20,260,278 bp
  • T to A, chromosome 9 at 36,696,560 bp
  • A to T, chromosome 9 at 59,979,777 bp
  • A to T, chromosome 9 at 110,726,454 bp
  • A to G, chromosome 9 at 119,694,127 bp
  • A to G, chromosome 10 at 24,109,105 bp
  • A to C, chromosome 10 at 29,196,724 bp
  • T to C, chromosome 10 at 117,004,511 bp
  • C to T, chromosome 11 at 6,620,947 bp
  • C to T, chromosome 11 at 69,602,732 bp
  • C to T, chromosome 11 at 95,028,582 bp
  • T to C, chromosome 11 at 102,437,536 bp
  • T to G, chromosome 12 at 70,984,565 bp
  • G to A, chromosome 12 at 85,122,140 bp
  • C to T, chromosome 12 at 99,894,746 bp
  • T to C, chromosome 12 at 112,657,091 bp
  • T to C, chromosome 13 at 98,789,767 bp
  • T to C, chromosome 14 at 51,195,835 bp
  • C to T, chromosome 14 at 56,082,336 bp
  • T to G, chromosome 15 at 12,926,029 bp
  • G to A, chromosome 15 at 42,523,520 bp
  • G to T, chromosome 16 at 10,545,532 bp
  • T to C, chromosome 16 at 16,462,009 bp
  • A to T, chromosome 16 at 58,824,433 bp
  • T to C, chromosome 16 at 59,314,850 bp
  • T to C, chromosome 17 at 25,829,674 bp
  • T to A, chromosome 17 at 37,049,761 bp
  • A to G, chromosome 17 at 71,243,372 bp
  • T to C, chromosome 17 at 84,749,140 bp
  • G to T, chromosome 18 at 31,971,450 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5457 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043020-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.