Strain Name:
C57BL/6J-MtgxR5465Btlr/Mmmh
Stock Number:
043026-MU
Citation ID:
RRID:MMRRC_043026-MU
Other Names:
R5465 (G1), C57BL/6J-MtgxR5465Btlr
Major Collection:

Gene Information

Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 74244
Homologene: 4662
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11287
Homologene: 104112
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, BB001228, Cxxc6, D10Ertd17e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52463
Homologene: 12735
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74996
Homologene: 9929
Gtf2a1
Name: general transcription factor II A, 1
Synonyms: TfIIAa/b, Tfiia1, 19kDa, 37kDa, 6330549H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 83602
HGNC: HGNC:4646
Homologene: 56331
Ralgapb
Name: Ral GTPase activating protein, beta subunit (non-catalytic)
Synonyms: B230339M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228850
Homologene: 10666
Slx4ip
Name: SLX4 interacting protein
Synonyms: 2410004I22Rik, 2210009G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 74243
Homologene: 49913
Utp6
Name: UTP6 small subunit processome component
Synonyms: HCA66, 4732497O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216987
Homologene: 41265
Arfgef1
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Synonyms: D130059B05Rik, BIG1, ARFGEP1, P200, D730028O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 211673
Homologene: 4687
Ncapd2
Name: non-SMC condensin I complex, subunit D2
Synonyms: 2810465G24Rik, 2810406C15Rik, CNAP1, CAP-D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68298
Homologene: 6497
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, b2b1200.1Clo, Drad-1, E130320P19Rik, E030015M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20563
Homologene: 3516
Rtn4r
Name: reticulon 4 receptor
Synonyms: NgR1, NgR, Nogo-66 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 65079
Homologene: 11299
Map3k1
Name: mitogen-activated protein kinase kinase kinase 1
Synonyms: Mekk, MEKK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 26401
HGNC: HGNC:6848
Homologene: 8056
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 20743
VEGA: 19
Homologene: 48482
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237553
Homologene: 75007
Ift172
Name: intraflagellar transport 172
Synonyms: avc1, wim, 4930553F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 67661
Homologene: 15202
Aplp1
Name: amyloid beta (A4) precursor-like protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11803
HGNC: HGNC:597
Homologene: 68447
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76295
Homologene: 32919
Khdrbs2
Name: KH domain containing, RNA binding, signal transduction associated 2
Synonyms: SLM-1, 6330586C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 170771
Homologene: 15772
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, Cav2.2, alpha(1B)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Wdr78
Name: WD repeat domain 78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242584
Homologene: 11702
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: Mll2, Mll4, C430014K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Map1a
Name: microtubule-associated protein 1 A
Synonyms: Mtap1, 6330416M19Rik, Mtap-1, Mtap1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17754
HGNC: HGNC:6835
Homologene: 1778
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Tbc1d16
Name: TBC1 domain family, member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 207592
Homologene: 10380
Ccna1
Name: cyclin A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12427
HGNC: HGNC:1577
Homologene: 31203
Vcpip1
Name: valosin containing protein (p97)/p47 complex interacting protein 1
Synonyms: Vcip135, 5730538E15Rik, 5730421J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70675
Homologene: 11814
Olfr561
Name: olfactory receptor 561
Synonyms: MOR14-2, GA_x6K02T2PBJ9-5491151-5492095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 259096
Homologene: 133029
Paxx
Name: non-homologous end joining factor
Synonyms: D930050G13Rik, paralog of XRCC4 and XLF, BC029214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227622
Homologene: 17788
Clic3
Name: chloride intracellular channel 3
Synonyms: 2300003G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 69454
HGNC: HGNC:2064
Homologene: 3432
Akr1b10
Name: aldo-keto reductase family 1, member B10 (aldose reductase)
Synonyms: 2310005E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67861
Homologene: 128412
Pcdhb6
Name: protocadherin beta 6
Synonyms: Pcdhb5B, PcdhbF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93877
Homologene: 62177
Olfr1463
Name: olfactory receptor 1463
Synonyms: MOR202-29P, GA_x6K02T2RE5P-3560863-3561795
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258120
HGNC: HGNC:8324
Homologene: 133886
Gpr162
Name: G protein-coupled receptor 162
Synonyms: Grca, A-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14788
Homologene: 8400
Cplx2
Name: complexin 2
Synonyms: 921-L, Gm34843
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 12890
HGNC: HGNC:2310
Homologene: 38212
Gnb2
Name: guanine nucleotide binding protein (G protein), beta 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14693
HGNC: HGNC:4398
Homologene: 68451
Zfp703
Name: zinc finger protein 703
Synonyms: Zeppo1, Csmn1, End2, 1110032O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 353310
Homologene: 81391
Olfr1356
Name: olfactory receptor 1356
Synonyms: MOR142-1, GA_x6K02T2QGN0-2962025-2962987, MOR142-2_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258305
HGNC: HGNC:8374
Homologene: 71958
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 9,747,147 bp
  • T to C, chromosome 1 at 10,159,534 bp
  • T to C, chromosome 1 at 32,619,174 bp
  • C to T, chromosome 2 at 24,650,426 bp
  • C to A, chromosome 2 at 25,458,496 bp
  • C to T, chromosome 2 at 25,459,761 bp
  • T to C, chromosome 2 at 121,306,025 bp
  • T to G, chromosome 2 at 137,004,947 bp
  • C to A, chromosome 2 at 158,448,405 bp
  • G to A, chromosome 3 at 35,810,184 bp
  • T to C, chromosome 3 at 55,045,644 bp
  • T to A, chromosome 4 at 103,049,561 bp
  • A to T, chromosome 5 at 31,261,518 bp
  • C to T, chromosome 5 at 48,249,912 bp
  • A to G, chromosome 5 at 137,528,513 bp
  • G to A, chromosome 6 at 34,388,918 bp
  • G to A, chromosome 6 at 114,652,532 bp
  • A to T, chromosome 6 at 124,861,171 bp
  • A to T, chromosome 6 at 125,176,783 bp
  • A to G, chromosome 6 at 128,486,961 bp
  • T to C, chromosome 7 at 30,436,852 bp
  • A to G, chromosome 7 at 102,775,433 bp
  • C to T, chromosome 7 at 112,059,002 bp
  • C to T, chromosome 8 at 26,979,205 bp
  • G to A, chromosome 8 at 125,760,911 bp
  • T to C, chromosome 10 at 62,839,777 bp
  • T to C, chromosome 10 at 78,847,018 bp
  • G to A, chromosome 10 at 114,588,442 bp
  • A to T, chromosome 11 at 79,949,010 bp
  • G to A, chromosome 11 at 119,156,059 bp
  • A to C, chromosome 12 at 91,567,791 bp
  • G to A, chromosome 13 at 54,379,539 bp
  • A to T, chromosome 13 at 111,756,120 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • A to T, chromosome 16 at 18,151,427 bp
  • A to T, chromosome 18 at 37,334,730 bp
  • C to A, chromosome 19 at 4,750,105 bp
  • T to C, chromosome 19 at 13,234,688 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5465 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043026-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.