Strain Name:
C57BL/6J-MtgxR5469Btlr/Mmmh
Stock Number:
043030-MU
Citation ID:
RRID:MMRRC_043030-MU
Other Names:
R5469 (G1), C57BL/6J-MtgxR5469Btlr
Major Collection:

Gene Information

Ccne2
Name: cyclin E2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12448
HGNC: HGNC:1590
Homologene: 7660
Ctnna1
Name: catenin (cadherin associated protein), alpha 1
Synonyms: alpha(E)-catenin, Catna1, 2010010M04Rik, alpha E catenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Map1b
Name: microtubule-associated protein 1B
Synonyms: Mtap5, Mtap-5, MAP5, Mtap1b, LC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Prtg
Name: protogenin
Synonyms: Igdcc5, A230098A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235472
Homologene: 54453
Ktn1
Name: kinectin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 16709
HGNC: HGNC:6467
Homologene: 7397
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 239188
VEGA: 14
Homologene: 56793
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: D3Ertd775e, Lba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Hnrnpr
Name: heterogeneous nuclear ribonucleoprotein R
Synonyms: hnRNPR, 2610528B01Rik, 2610003J05Rik, Hnrpr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74326
HGNC: HGNC:5047
Homologene: 4251
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Mcpr, Apc1, 2610021O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17222
Homologene: 7414
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 78586
VEGA: 17
Homologene: 9994
Tlk1
Name: tousled-like kinase 1
Synonyms: 4930545J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228012
Homologene: 130657
Mphosph10
Name: M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
Synonyms: 5730405D16Rik, 2810453H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67973
HGNC: HGNC:7213
Homologene: 134741
Casr
Name: calcium-sensing receptor
Synonyms: CaR, cation sensing receptor, Gprc2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Rrn3
Name: RRN3 RNA polymerase I transcription factor homolog (yeast)
Synonyms: TIF-1A, E130302O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 106298
VEGA: 16
Homologene: 39531
Pdcd10
Name: programmed cell death 10
Synonyms: CCM3, TF-1 cell apoptosis related protein-15, Tfa15, 2410003B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 56426
HGNC: HGNC:8761
Homologene: 10505
Jak3
Name: Janus kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 16453
HGNC: HGNC:6193
Homologene: 181
Casp2
Name: caspase 2
Synonyms: Caspase-2, Ich-1, Nedd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 12366
HGNC: HGNC:1503
Homologene: 7254
Dhx29
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
Synonyms: E130202M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218629
VEGA: 13
Homologene: 10387
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: Flk2, wmfl, Flt-3, CD135, Flk-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Chst10
Name: carbohydrate sulfotransferase 10
Synonyms: ST, Hnk-1st
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 98388
Homologene: 21013
Slc35e2
Name: solute carrier family 35, member E2
Synonyms: A530082C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320541
Homologene: 84987
Lama2
Name: laminin, alpha 2
Synonyms: mer, merosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: Fam38b, Piezo2, 9030411M15Rik, Fam38b2, 9430028L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 667742
Homologene: 49695
Cd180
Name: CD180 antigen
Synonyms: RP105, Ly78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17079
HGNC: HGNC:6726
Homologene: 4077
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: A830008O07Rik, 1700010C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 70363
VEGA: 15
Homologene: 66605
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 212167
Homologene: 45504
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Tfip11
Name: tuftelin interacting protein 11
Synonyms: Tip39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 54723
Homologene: 40813
Vmn2r77
Name: vomeronasal 2, receptor 77
Synonyms: EG546983
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 546983
Homologene: 115466
Pold2
Name: polymerase (DNA directed), delta 2, regulatory subunit
Synonyms: p50, 50kDa, po1D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18972
HGNC: HGNC:9176
Homologene: 4538
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 26564
Homologene: 55831
Tecpr1
Name: tectonin beta-propeller repeat containing 1
Synonyms: 2210010N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 70381
Homologene: 9120
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Adam7
Name: a disintegrin and metallopeptidase domain 7
Synonyms: EAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 11500
HGNC: HGNC:214
Homologene: 2830
Traf1
Name: TNF receptor-associated factor 1
Synonyms: 4732496E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22029
Homologene: 4138
Rad51ap1
Name: RAD51 associated protein 1
Synonyms: RAB22, 2510006L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 19362
Homologene: 48436
C9
Name: complement component 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 12279
HGNC: HGNC:1358
Homologene: 74406
Usp9y
Name: ubiquitin specific peptidase 9, Y chromosome
Synonyms: Fafl2, Dffry
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
Alteration at locus: Chemically Induced
NCBI: 107868
Homologene: 68408
Ctsh
Name: cathepsin H
Synonyms: Cat H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 13036
HGNC: HGNC:2535
Homologene: 36159
V1ra8
Name: vomeronasal 1 receptor, A8
Synonyms: Vmn1r-ps33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 113850
Sstr5
Name: somatostatin receptor 5
Synonyms: sst5, Smstr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20609
VEGA: 17
Homologene: 20287
Slc30a3
Name: solute carrier family 30 (zinc transporter), member 3
Synonyms: Znt3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 22784
Homologene: 74470
Ryk
Name: receptor-like tyrosine kinase
Synonyms: Vik, ERK-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20187
Homologene: 68287
Gm12689
Name: predicted gene 12689
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Allc
Name: allantoicase
Synonyms: 1700012B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 94041
Homologene: 6202
Rfk
Name: riboflavin kinase
Synonyms: 0610038L10Rik, ATP:riboflavin 5'-phosphotransferase, flavokinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 54391
Homologene: 115690
Gm20997
Name: predicted gene, 20997
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
Ear-ps2
Name: eosinophil-associated, ribonuclease A family, pseudogene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 93743
8030451A03Rik
Name: RIKEN cDNA 8030451A03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100504061
RP23-214I6.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Trav12-1
Name: T cell receptor alpha variable 12-1
Synonyms: Gm17011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 630086
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 38,865,527 bp
  • T to C, chromosome 1 at 154,443,937 bp
  • G to A, chromosome 2 at 34,956,325 bp
  • T to C, chromosome 2 at 70,721,668 bp
  • G to T, chromosome 2 at 128,675,701 bp
  • T to A, chromosome 3 at 75,521,057 bp
  • C to T, chromosome 3 at 86,542,641 bp
  • A to T, chromosome 4 at 11,201,353 bp
  • T to C, chromosome 4 at 64,013,925 bp
  • T to C, chromosome 4 at 99,296,165 bp
  • C to T, chromosome 4 at 136,319,434 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • A to T, chromosome 5 at 21,290,544 bp
  • T to A, chromosome 5 at 31,088,660 bp
  • G to A, chromosome 5 at 112,334,325 bp
  • G to A, chromosome 5 at 123,037,312 bp
  • G to A, chromosome 5 at 144,206,558 bp
  • A to T, chromosome 5 at 147,355,083 bp
  • C to A, chromosome 6 at 42,269,334 bp
  • C to T, chromosome 6 at 90,203,204 bp
  • C to T, chromosome 6 at 126,928,227 bp
  • A to C, chromosome 6 at 130,483,786 bp
  • A to T, chromosome 7 at 64,389,445 bp
  • A to T, chromosome 7 at 86,802,063 bp
  • A to G, chromosome 8 at 71,678,773 bp
  • A to T, chromosome 9 at 72,891,965 bp
  • G to T, chromosome 9 at 90,060,511 bp
  • A to G, chromosome 9 at 102,906,954 bp
  • G to A, chromosome 10 at 27,041,189 bp
  • G to A, chromosome 11 at 5,873,048 bp
  • C to T, chromosome 11 at 115,986,764 bp
  • A to T, chromosome 12 at 28,555,306 bp
  • T to C, chromosome 13 at 53,117,339 bp
  • C to T, chromosome 13 at 99,429,338 bp
  • T to A, chromosome 13 at 102,704,834 bp
  • C to T, chromosome 13 at 112,944,539 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • A to T, chromosome 14 at 47,690,920 bp
  • A to G, chromosome 14 at 53,538,473 bp
  • T to A, chromosome 14 at 68,509,884 bp
  • A to G, chromosome 14 at 77,592,974 bp
  • T to C, chromosome 15 at 6,454,283 bp
  • T to A, chromosome 15 at 71,446,043 bp
  • A to G, chromosome 16 at 13,813,100 bp
  • A to G, chromosome 16 at 36,510,030 bp
  • A to C, chromosome 17 at 25,492,069 bp
  • C to A, chromosome 17 at 86,119,942 bp
  • T to A, chromosome 18 at 35,239,520 bp
  • A to T, chromosome 18 at 63,027,864 bp
  • T to A, chromosome 19 at 17,395,202 bp
  • G to A, chromosome Y at 1,364,714 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043030-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.