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Strain Name:
C57BL/6J-MtgxR5469Btlr/Mmmh
Stock Number:
043030-MU
Citation ID:
RRID:MMRRC_043030-MU
Other Names:
R5469 (G1), C57BL/6J-MtgxR5469Btlr
Major Collection:

Strain Information

Ctnna1
Name: catenin alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik, catenin (cadherin associated protein), alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Prtg
Name: protogenin
Synonyms: A230098A12Rik, Igdcc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235472
Homologene: 54453
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 38,865,527 bp
  • T to C, chromosome 1 at 154,443,937 bp
  • G to A, chromosome 2 at 34,956,325 bp
  • T to C, chromosome 2 at 70,721,668 bp
  • G to T, chromosome 2 at 128,675,701 bp
  • T to A, chromosome 3 at 75,521,057 bp
  • C to T, chromosome 3 at 86,542,641 bp
  • A to T, chromosome 4 at 11,201,353 bp
  • T to C, chromosome 4 at 64,013,925 bp
  • T to C, chromosome 4 at 99,296,165 bp
  • C to T, chromosome 4 at 136,319,434 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • A to T, chromosome 5 at 21,290,544 bp
  • T to A, chromosome 5 at 31,088,660 bp
  • G to A, chromosome 5 at 112,334,325 bp
  • G to A, chromosome 5 at 123,037,312 bp
  • G to A, chromosome 5 at 144,206,558 bp
  • A to T, chromosome 5 at 147,355,083 bp
  • C to A, chromosome 6 at 42,269,334 bp
  • C to T, chromosome 6 at 90,203,204 bp
  • C to T, chromosome 6 at 126,928,227 bp
  • A to C, chromosome 6 at 130,483,786 bp
  • A to T, chromosome 7 at 64,389,445 bp
  • A to T, chromosome 7 at 86,802,063 bp
  • A to G, chromosome 8 at 71,678,773 bp
  • A to T, chromosome 9 at 72,891,965 bp
  • G to T, chromosome 9 at 90,060,511 bp
  • A to G, chromosome 9 at 102,906,954 bp
  • G to A, chromosome 10 at 27,041,189 bp
  • G to A, chromosome 11 at 5,873,048 bp
  • C to T, chromosome 11 at 115,986,764 bp
  • A to T, chromosome 12 at 28,555,306 bp
  • T to C, chromosome 13 at 53,117,339 bp
  • C to T, chromosome 13 at 99,429,338 bp
  • T to A, chromosome 13 at 102,704,834 bp
  • C to T, chromosome 13 at 112,944,539 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • A to T, chromosome 14 at 47,690,920 bp
  • A to G, chromosome 14 at 53,538,473 bp
  • T to A, chromosome 14 at 68,509,884 bp
  • A to G, chromosome 14 at 77,592,974 bp
  • T to C, chromosome 15 at 6,454,283 bp
  • T to A, chromosome 15 at 71,446,043 bp
  • A to G, chromosome 16 at 13,813,100 bp
  • A to G, chromosome 16 at 36,510,030 bp
  • A to C, chromosome 17 at 25,492,069 bp
  • C to A, chromosome 17 at 86,119,942 bp
  • T to A, chromosome 18 at 35,239,520 bp
  • A to T, chromosome 18 at 63,027,864 bp
  • T to A, chromosome 19 at 17,395,202 bp
  • G to A, chromosome Y at 1,364,714 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043030-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.