Strain Name:
C57BL/6J-MtgxR5469Btlr/Mmmh
Stock Number:
043030-MU
Citation ID:
RRID:MMRRC_043030-MU
Other Names:
R5469 (G1), C57BL/6J-MtgxR5469Btlr
Major Collection:

Strain Information

Ccne2
Name: cyclin E2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12448
HGNC: HGNC:1590
Homologene: 7660
Ctnna1
Name: catenin alpha 1
Synonyms: catenin (cadherin associated protein), alpha 1, 2010010M04Rik, alpha E catenin, alpha(E)-catenin, Catna1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Map1b
Name: microtubule-associated protein 1B
Synonyms: Mtap1b, Mtap5, LC1, MAP5, Mtap-5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Prtg
Name: protogenin
Synonyms: Igdcc5, A230098A12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235472
Homologene: 54453
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Hnrnpr
Name: heterogeneous nuclear ribonucleoprotein R
Synonyms: hnRNPR, Hnrpr, 2610003J05Rik, 2610528B01Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74326
HGNC: HGNC:5047
Homologene: 4251
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, 2610021O03Rik, Apc1, Mcpr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17222
Homologene: 7414
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78586
VEGA: 17
Homologene: 9994
Tlk1
Name: tousled-like kinase 1
Synonyms: 4930545J15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228012
Homologene: 130657
Mphosph10
Name: M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
Synonyms: 5730405D16Rik, 2810453H10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67973
HGNC: HGNC:7213
Homologene: 134741
Casr
Name: calcium-sensing receptor
Synonyms: CaR, Gprc2a, cation sensing receptor
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Rrn3
Name: RRN3 RNA polymerase I transcription factor homolog (yeast)
Synonyms: E130302O19Rik, TIF-1A
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106298
VEGA: 16
Homologene: 39531
Pdcd10
Name: programmed cell death 10
Synonyms: TF-1 cell apoptosis related protein-15, Tfa15, 2410003B13Rik, CCM3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56426
HGNC: HGNC:8761
Homologene: 10505
Jak3
Name: Janus kinase 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16453
HGNC: HGNC:6193
Homologene: 181
Casp2
Name: caspase 2
Synonyms: Caspase-2, Nedd2, Ich-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12366
HGNC: HGNC:1503
Homologene: 7254
Dhx29
Name: DExH-box helicase 29
Synonyms: E130202M19Rik, DEAH (Asp-Glu-Ala-His) box polypeptide 29
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218629
VEGA: 13
Homologene: 10387
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: wmfl, Flk-2, Flk2, Flt-3, CD135
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Chst10
Name: carbohydrate sulfotransferase 10
Synonyms: ST, Hnk-1st
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98388
Homologene: 21013
Slc35e2
Name: solute carrier family 35, member E2
Synonyms: A530082C11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320541
Homologene: 84987
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: Piezo2, Fam38b, 9030411M15Rik, Fam38b2, 9430028L06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 667742
Homologene: 49695
Cd180
Name: CD180 antigen
Synonyms: Ly78, RP105
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17079
HGNC: HGNC:6726
Homologene: 4077
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: 1700010C24Rik, A830008O07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212167
Homologene: 45504
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Tfip11
Name: tuftelin interacting protein 11
Synonyms: Tip39
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 54723
Homologene: 40813
Vmn2r77
Name: vomeronasal 2, receptor 77
Synonyms: EG546983
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546983
Homologene: 115466
Pold2
Name: polymerase (DNA directed), delta 2, regulatory subunit
Synonyms: 50kDa, po1D2, p50
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18972
HGNC: HGNC:9176
Homologene: 4538
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26564
Homologene: 55831
Tecpr1
Name: tectonin beta-propeller repeat containing 1
Synonyms: 2210010N04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70381
Homologene: 9120
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Adam7
Name: a disintegrin and metallopeptidase domain 7
Synonyms: EAP1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11500
HGNC: HGNC:214
Homologene: 2830
Traf1
Name: TNF receptor-associated factor 1
Synonyms: 4732496E14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22029
Homologene: 4138
Rad51ap1
Name: RAD51 associated protein 1
Synonyms: 2510006L10Rik, RAB22
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19362
Homologene: 48436
C9
Name: complement component 9
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12279
HGNC: HGNC:1358
Homologene: 74406
Usp9y
Name: ubiquitin specific peptidase 9, Y chromosome
Synonyms: Dffry, Fafl2
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 107868
Homologene: 68408
Ctsh
Name: cathepsin H
Synonyms: Cat H
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13036
HGNC: HGNC:2535
Homologene: 36159
V1ra8
Name: vomeronasal 1 receptor, A8
Synonyms: Vmn1r-ps33
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113850
Sstr5
Name: somatostatin receptor 5
Synonyms: Smstr5, sst5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20609
VEGA: 17
Homologene: 20287
Slc30a3
Name: solute carrier family 30 (zinc transporter), member 3
Synonyms: Znt3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22784
Homologene: 74470
Ryk
Name: receptor-like tyrosine kinase
Synonyms: Vik, ERK-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20187
Homologene: 68287
Gm12689
Name: predicted gene 12689
Type: Gene
Species: Mouse
Chromosome: 4
Allc
Name: allantoicase
Synonyms: 1700012B22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 94041
Homologene: 6202
Rfk
Name: riboflavin kinase
Synonyms: ATP:riboflavin 5'-phosphotransferase, flavokinase, 0610038L10Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54391
Homologene: 115690
Gm20997
Name: predicted gene, 20997
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Ear-ps2
Name: eosinophil-associated, ribonuclease A family, pseudogene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93743
8030451A03Rik
Name: RIKEN cDNA 8030451A03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100504061
RP23-214I6.4
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Trav12-1
Name: T cell receptor alpha variable 12-1
Synonyms: Gm17011
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 630086
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 38,865,527 bp
  • T to C, chromosome 1 at 154,443,937 bp
  • G to A, chromosome 2 at 34,956,325 bp
  • T to C, chromosome 2 at 70,721,668 bp
  • G to T, chromosome 2 at 128,675,701 bp
  • T to A, chromosome 3 at 75,521,057 bp
  • C to T, chromosome 3 at 86,542,641 bp
  • A to T, chromosome 4 at 11,201,353 bp
  • T to C, chromosome 4 at 64,013,925 bp
  • T to C, chromosome 4 at 99,296,165 bp
  • C to T, chromosome 4 at 136,319,434 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • A to T, chromosome 5 at 21,290,544 bp
  • T to A, chromosome 5 at 31,088,660 bp
  • G to A, chromosome 5 at 112,334,325 bp
  • G to A, chromosome 5 at 123,037,312 bp
  • G to A, chromosome 5 at 144,206,558 bp
  • A to T, chromosome 5 at 147,355,083 bp
  • C to A, chromosome 6 at 42,269,334 bp
  • C to T, chromosome 6 at 90,203,204 bp
  • C to T, chromosome 6 at 126,928,227 bp
  • A to C, chromosome 6 at 130,483,786 bp
  • A to T, chromosome 7 at 64,389,445 bp
  • A to T, chromosome 7 at 86,802,063 bp
  • A to G, chromosome 8 at 71,678,773 bp
  • A to T, chromosome 9 at 72,891,965 bp
  • G to T, chromosome 9 at 90,060,511 bp
  • A to G, chromosome 9 at 102,906,954 bp
  • G to A, chromosome 10 at 27,041,189 bp
  • G to A, chromosome 11 at 5,873,048 bp
  • C to T, chromosome 11 at 115,986,764 bp
  • A to T, chromosome 12 at 28,555,306 bp
  • T to C, chromosome 13 at 53,117,339 bp
  • C to T, chromosome 13 at 99,429,338 bp
  • T to A, chromosome 13 at 102,704,834 bp
  • C to T, chromosome 13 at 112,944,539 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • A to T, chromosome 14 at 47,690,920 bp
  • A to G, chromosome 14 at 53,538,473 bp
  • T to A, chromosome 14 at 68,509,884 bp
  • A to G, chromosome 14 at 77,592,974 bp
  • T to C, chromosome 15 at 6,454,283 bp
  • T to A, chromosome 15 at 71,446,043 bp
  • A to G, chromosome 16 at 13,813,100 bp
  • A to G, chromosome 16 at 36,510,030 bp
  • A to C, chromosome 17 at 25,492,069 bp
  • C to A, chromosome 17 at 86,119,942 bp
  • T to A, chromosome 18 at 35,239,520 bp
  • A to T, chromosome 18 at 63,027,864 bp
  • T to A, chromosome 19 at 17,395,202 bp
  • G to A, chromosome Y at 1,364,714 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043030-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.