Strain Name:
C57BL/6J-MtgxR5470Btlr/Mmmh
Stock Number:
043031-MU
Citation ID:
RRID:MMRRC_043031-MU
Other Names:
R5470 (G1), C57BL/6J-MtgxR5470Btlr
Major Collection:

Gene Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: D630042G03Rik, Nkcc2, urehr3, mBSC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20495
Homologene: 286
Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, 2700090M07Rik, D230019K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Rcan2
Name: regulator of calcineurin 2
Synonyms: ZAKI-4, Dscr1l1, MCIP2, Csp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 53901
VEGA: 17
HGNC: HGNC:3041
Homologene: 130985
Strn
Name: striatin, calmodulin binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268980
Homologene: 2380
Utp20
Name: UTP20 small subunit processome component
Synonyms: 3830408P06Rik, mDRIM, DRIM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: C920009D07Rik, TAPP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101476
Homologene: 11001
Pcm1
Name: pericentriolar material 1
Synonyms: C030044G17Rik, 9430077F19Rik, 2600002H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
En2
Name: engrailed 2
Synonyms: En-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13799
HGNC: HGNC:3343
Homologene: 55579
Cop1
Name: COP1, E3 ubiquitin ligase
Synonyms: Rfwd2, Cop1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26374
Homologene: 115565
Bcl2l13
Name: BCL2-like 13 (apoptosis facilitator)
Synonyms: Mil1, E430016C20Rik, Mil-1, BCL-RAMBO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94044
Homologene: 9111
Etl4
Name: enhancer trap locus 4
Synonyms: Skt, 9430077C05Rik, 6620402G01Rik, E330027G05Rik, Sickle tail, Etl-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Nup133
Name: nucleoporin 133
Synonyms: 4832420O05Rik, mermaid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234865
Homologene: 32402
Fndc3a
Name: fibronectin type III domain containing 3A
Synonyms: F730017H24Rik, 1700094E19Rik, sys, Fndc3, D14Ertd453e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319448
Homologene: 8952
Actn1
Name: actinin, alpha 1
Synonyms: 3110023F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109711
VEGA: 12
HGNC: HGNC:163
Homologene: 55553
Palb2
Name: partner and localizer of BRCA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233826
Homologene: 11652
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: Duplin, 5830451P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Aqr
Name: aquarius
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11834
Homologene: 7629
Atp6v1d
Name: ATPase, H+ transporting, lysosomal V1 subunit D
Synonyms: VATD, 1110004P10Rik, Atp6m, Vma8, lysosomal 34kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73834
VEGA: 12
Homologene: 5783
Tsn
Name: translin
Synonyms: 2610034C24Rik, TB-RBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22099
Homologene: 3397
Prkcq
Name: protein kinase C, theta
Synonyms: PKC-0, Pkcq, PKC theta, PKC-theta, A130035A12Rik, PKCtheta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18761
HGNC: HGNC:9410
Homologene: 21263
Neo1
Name: neogenin
Synonyms: Igdcc2, 2610028H22Rik, D930014N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18007
VEGA: 9
HGNC: HGNC:7754
Homologene: 1870
Lrch3
Name: leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms: LOC385628, 2210409B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70144
Homologene: 13111
Gorab
Name: golgin, RAB6-interacting
Synonyms: NTKL-BP1, Scyl1bp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98376
Homologene: 45113
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: 8030460J03Rik, BACH1, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237911
Homologene: 32766
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: 2010013B10Rik, D330050P16Rik, A230048G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Nsl1
Name: NSL1, MIS12 kinetochore complex component
Synonyms: LOC381318, 4833432M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381318
Homologene: 22898
Gpr152
Name: G protein-coupled receptor 152
Synonyms: LOC269053, A930009H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 269053
VEGA: 19
Homologene: 35474
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546144
Homologene: 52326
Krt74
Name: keratin 74
Synonyms: Kb37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406222
VEGA: 15
Slc35e2
Name: solute carrier family 35, member E2
Synonyms: A530082C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320541
Homologene: 84987
Ttn
Name: titin
Synonyms: 2310057K23Rik, connectin, D830007G01Rik, D330041I19Rik, L56, shru, 1100001C23Rik, 2310036G12Rik, mdm, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Zhx2
Name: zinc fingers and homeoboxes 2
Synonyms: Raf, Afr1, Afr-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 387609
Homologene: 8968
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Slc22a29
Name: solute carrier family 22. member 29
Synonyms: D630002G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 236293
Homologene: 77136
Deup1
Name: deuterosome assembly protein 1
Synonyms: Ccdc67, 4933401K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234964
Homologene: 18760
Tbx3
Name: T-box 3
Synonyms: D5Ertd189e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21386
Homologene: 4371
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Pold2
Name: polymerase (DNA directed), delta 2, regulatory subunit
Synonyms: p50, 50kDa, po1D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18972
HGNC: HGNC:9176
Homologene: 4538
Fetub
Name: fetuin beta
Synonyms: 2310011O17Rik, D17980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 59083
VEGA: 16
HGNC: HGNC:3658
Homologene: 8660
Pitrm1
Name: pitrilysin metallepetidase 1
Synonyms: Ntup1, PreP, MP-1, 2310012C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 69617
VEGA: 13
Homologene: 5742
Slc4a10
Name: solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
Synonyms: NCBE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94229
Homologene: 23340
Cyp2c39
Name: cytochrome P450, family 2, subfamily c, polypeptide 39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13098
HGNC: HGNC:2622
Homologene: 117948
Pfkfb4
Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270198
HGNC: HGNC:8875
Homologene: 48288
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Gm14129
Name: predicted gene 14129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Homologene: 141190
Nmrk1
Name: nicotinamide riboside kinase 1
Synonyms: BC016495, D630020N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225994
Homologene: 6787
Il6ra
Name: interleukin 6 receptor, alpha
Synonyms: CD126, IL-6R, Il6r, IL-6 receptor alpha chain
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16194
HGNC: HGNC:6019
Homologene: 474
Ccdc7b
Name: coiled-coil domain containing 7B
Synonyms: 1700008F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 75453
Homologene: 49919
Naalad2
Name: N-acetylated alpha-linked acidic dipeptidase 2
Synonyms: NAADALASE2, Folh1b, GCP3, GCPIII, D9Ertd285e, NAALADASE2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72560
Homologene: 3294
Hsd17b3
Name: hydroxysteroid (17-beta) dehydrogenase 3
Synonyms: 17(beta)HSD type 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 15487
VEGA: 13
HGNC: HGNC:5212
Homologene: 20089
Mgarp
Name: mitochondria localized glutamic acid rich protein
Synonyms: Osap, 4930583H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67749
Homologene: 49858
Vmn1r45
Name: vomeronasal 1 receptor 45
Synonyms: V1r2, V1ra2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22297
Homologene: 130651
Cyp3a57
Name: cytochrome P450, family 3, subfamily a, polypeptide 57
Synonyms: EG622127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622127
Homologene: 135775
Tex14
Name: testis expressed gene 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 83560
Homologene: 12838
Fignl1
Name: fidgetin-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 60530
Homologene: 11063
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 81840
Homologene: 56899
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Olfr23
Name: olfactory receptor 23
Synonyms: MTPCR50, GA_x6K02T2P1NL-4097159-4098136, MOR135-27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18321
Homologene: 115483
Spam1
Name: sperm adhesion molecule 1
Synonyms: Ph-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20690
Homologene: 7547
Slc22a8
Name: solute carrier family 22 (organic anion transporter), member 8
Synonyms: mOat3, OAT3, Roct
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19879
VEGA: 19
Homologene: 20901
Endou
Name: endonuclease, polyU-specific
Synonyms: Pp11r, Tcl-30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19011
VEGA: 15
Homologene: 48394
Zfpm1
Name: zinc finger protein, multitype 1
Synonyms: Friend of GATA-1, Fog1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22761
Homologene: 7606
Tjp3
Name: tight junction protein 3
Synonyms: ZO-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 27375
VEGA: 10
Homologene: 8458
Ndufaf3
Name: NADH:ubiquinone oxidoreductase complex assembly factor 3
Synonyms: 4733401H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66706
Homologene: 32460
Gdf9
Name: growth differentiation factor 9
Synonyms: Gdf-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14566
HGNC: HGNC:4224
Homologene: 3851
Olfr610
Name: olfactory receptor 610
Synonyms: MOR9-2, GA_x6K02T2PBJ9-6221839-6220892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259085
Homologene: 17495
Opalin
Name: oligodendrocytic myelin paranodal and inner loop protein
Synonyms: Tmp10, Tmem10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226115
VEGA: 19
Homologene: 13237
Klk1b16
Name: kallikrein 1-related peptidase b16
Synonyms: mGk-16, Klk16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16615
HGNC: HGNC:6364
Homologene: 68141
Ly96
Name: lymphocyte antigen 96
Synonyms: myeloid differentiation factor-2, ESOP-1, MD-2, MD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17087
Homologene: 9109
Olfr399
Name: olfactory receptor 399
Synonyms: GA_x6K02T2P1NL-4211516-4210581, MOR255-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259006
Homologene: 27325
Slco4a1
Name: solute carrier organic anion transporter family, member 4a1
Synonyms: OATP-E, Slc21a12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108115
Homologene: 9482
Zfp689
Name: zinc finger protein 689
Synonyms: 4933416E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71131
Homologene: 16318
Ear-ps2
Name: eosinophil-associated, ribonuclease A family, pseudogene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93743
C530025M09Rik
Name: RIKEN cDNA C530025M09 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,709,486 bp
  • G to A, chromosome 1 at 118,311,019 bp
  • A to T, chromosome 1 at 159,266,860 bp
  • A to G, chromosome 1 at 163,392,509 bp
  • T to C, chromosome 1 at 191,080,540 bp
  • G to A, chromosome 2 at 11,260,721 bp
  • A to T, chromosome 2 at 20,529,980 bp
  • A to G, chromosome 2 at 52,249,438 bp
  • G to A, chromosome 2 at 62,289,948 bp
  • A to T, chromosome 2 at 76,729,864 bp
  • G to A, chromosome 2 at 114,157,575 bp
  • A to T, chromosome 2 at 125,170,714 bp
  • G to T, chromosome 2 at 148,927,817 bp
  • G to A, chromosome 2 at 149,831,125 bp
  • T to C, chromosome 2 at 180,474,114 bp
  • C to A, chromosome 3 at 51,391,285 bp
  • C to T, chromosome 3 at 89,885,995 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • C to T, chromosome 5 at 28,166,924 bp
  • A to T, chromosome 5 at 36,031,183 bp
  • A to G, chromosome 5 at 62,818,766 bp
  • C to T, chromosome 5 at 119,680,762 bp
  • A to G, chromosome 5 at 124,753,168 bp
  • A to G, chromosome 5 at 145,372,619 bp
  • T to A, chromosome 6 at 24,797,035 bp
  • T to C, chromosome 6 at 89,933,716 bp
  • G to T, chromosome 6 at 120,862,872 bp
  • A to T, chromosome 7 at 44,137,331 bp
  • C to T, chromosome 7 at 84,593,185 bp
  • T to G, chromosome 7 at 103,506,509 bp
  • C to A, chromosome 7 at 122,114,351 bp
  • C to T, chromosome 7 at 127,444,253 bp
  • T to C, chromosome 7 at 130,908,376 bp
  • T to A, chromosome 8 at 41,287,683 bp
  • A to G, chromosome 8 at 122,333,793 bp
  • T to C, chromosome 8 at 123,930,966 bp
  • T to A, chromosome 8 at 129,072,600 bp
  • T to C, chromosome 9 at 15,582,620 bp
  • T to A, chromosome 9 at 18,330,851 bp
  • G to T, chromosome 9 at 58,931,067 bp
  • A to T, chromosome 9 at 74,139,699 bp
  • T to C, chromosome 9 at 108,566,444 bp
  • T to A, chromosome 9 at 109,027,593 bp
  • T to C, chromosome 10 at 81,279,547 bp
  • T to A, chromosome 10 at 88,817,896 bp
  • G to A, chromosome 11 at 5,873,048 bp
  • T to A, chromosome 11 at 11,802,640 bp
  • T to A, chromosome 11 at 53,436,754 bp
  • T to C, chromosome 11 at 73,940,870 bp
  • A to G, chromosome 11 at 74,053,907 bp
  • T to C, chromosome 11 at 86,148,542 bp
  • A to G, chromosome 11 at 87,551,604 bp
  • G to A, chromosome 12 at 78,845,284 bp
  • T to A, chromosome 12 at 80,168,941 bp
  • T to C, chromosome 13 at 6,553,270 bp
  • G to T, chromosome 13 at 64,073,899 bp
  • G to A, chromosome 14 at 44,047,060 bp
  • A to G, chromosome 14 at 52,212,609 bp
  • A to G, chromosome 14 at 72,574,568 bp
  • G to T, chromosome 15 at 57,823,074 bp
  • G to T, chromosome 15 at 80,916,711 bp
  • A to G, chromosome 15 at 97,718,955 bp
  • G to A, chromosome 15 at 101,754,465 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • T to A, chromosome 16 at 32,998,590 bp
  • A to G, chromosome 17 at 34,716,973 bp
  • A to G, chromosome 17 at 43,836,283 bp
  • A to T, chromosome 17 at 78,656,945 bp
  • A to T, chromosome 17 at 78,821,579 bp
  • G to A, chromosome 19 at 4,143,129 bp
  • A to T, chromosome 19 at 8,161,516 bp
  • G to A, chromosome 19 at 8,607,870 bp
  • A to T, chromosome 19 at 18,639,884 bp
  • A to G, chromosome 19 at 39,513,530 bp
  • A to G, chromosome 19 at 41,066,531 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5470 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043031-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.